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5(6)-Aminofluorescein

5(6)-Aminofluorescein
5(6)-Aminofluorescein structure
CAS No.
27599-63-9
Chemical Name:
5(6)-Aminofluorescein
Synonyms
5(6)-AFM;FLUORESCEINAMINE;AMINOFLUORESCEIN;4-AMINOFLUORESCEIN;1-AMINOFLUORESCEIN;1-FLUORESCEINAMINE;5(6)-AMinofluorescei;5(6)-Aminofluorescein;FLUORESCEINAMINE ISOMER I;FLUORESCEINAMINE, ISOMER 1
CBNumber:
CB0254490
Molecular Formula:
C20H13NO5
Formula Weight:
347.32
MOL File:
27599-63-9.mol

5(6)-Aminofluorescein Properties

Melting point:
223 °C (dec.)(lit.)
Density 
1.64
storage temp. 
2-8°C
form 
powder
color 
red-brown
SAFETY
  • Risk and Safety Statements
Hazard Codes  Xn
Risk Statements  22-36/37/38
Safety Statements  26
WGK Germany  3
10
HS Code  29321900

5(6)-Aminofluorescein price

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 201626 Fluoresceinamine, isomer I 27599-63-9 250 mg $45.3 2021-12-16 Buy
Sigma-Aldrich 201626 Fluoresceinamine, isomer I 27599-63-9 1 g $116 2021-12-16 Buy
Sigma-Aldrich 201626 Fluoresceinamine, isomer I 27599-63-9 5 g $430 2021-12-16 Buy
TRC F537558 Fluoresceinamine 27599-63-9 2.5g $185 2021-12-16 Buy
Usbiological F5035 Fluoresceinamine, Isomer 1 27599-63-9 250mg $368 2021-12-16 Buy

5(6)-Aminofluorescein Chemical Properties,Uses,Production

Uses

Fluoresceinamine is a fluorescent labeling precursor used to prepare FITC.

Purification Methods

Dissolve it in EtOH, treat with charcoal, filter, evaporate and dry the residue in a vacuum at 100o overnight. Also recrystallise it from 6% HCl, then dissolve it in 0.5% aqueous NaOH and precipitate it by acidifying with acetic acid. The separate amines are made from the respective nitro compounds, which are best separated via their acetate salts. They have similar RF of 0.26 on Silica Gel Merck F254 in 5 mL MeOH + 150 mL Et2O saturated with H2O. IR (Me2SO) has a band at max 1690 cm-1 (CO2-) and sometimes a weak band at 1750 cm-1 due to the lactone. UV (EtOH) of the 6-max isomer has max at 222nm ( 60 000) and the 5-isomer at max 222nm ( 60 000) and 285nm ( 20.600). [IR: McKinney & Churchill J Chem Soc (C) 654 1970, McKinney et al. J Org Chem 27 3986 1962, UV: Verbiscar J Org Chem 29 490 1964, Beilstein 19 III/IV 4337, 19/8 V 713.]

5(6)-Aminofluorescein Preparation Products And Raw materials

Raw materials

Preparation Products


5(6)-Aminofluorescein Suppliers

Global( 88)Suppliers
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Target Molecule Corp
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