SPH I
- CAS No.
- 85270-15-1
- Chemical Name:
- SPH I
- Synonyms
- APA I;SPH I;Einecs 286-569-8;APA I RESTRICTION ENZYME;SPH I RESTRICTION ENZYME;restriction endonuclease Sph I;RESTRICTION ENDONUCLEASE APA I;RESTRICTION ENDONUCLEASES APA I;RESTRICTION ENDONUCLEASES SPH I;apa i from acetobacter pasteurianus
- CBNumber:
- CB0280961
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MFCD00166621
- MOL File:
- Mol file
SPH I price More Price(5)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Usbiological | A2298-63 | Apa I | 85270-15-1 | 5000U | $327 | 2021-12-16 | Buy |
| Biorbyt Ltd | orb93990 | Apa I | 85270-15-1 | 25000units | $486.2 | 2021-12-16 | Buy |
| Biorbyt Ltd | orb94025 | Sph I | 85270-15-1 | 250units | $74.8 | 2021-12-16 | Buy |
| Biorbyt Ltd | orb93990 | Apa I | 85270-15-1 | 5000units | $151.3 | 2021-12-16 | Buy |
| Biorbyt Ltd | orb94025 | Sph I | 85270-15-1 | 1250Units | $302.6 | 2021-12-16 | Buy |
SPH I Chemical Properties,Uses,Production
General Description
Compatible ends
Apa I ends are not compatible with those generated by any other known restriction enzymes.
Isoschizomers
Apa I is an isoschizomer to Bsp 120 I and Psp OMI.
Methylation sensitivity
Apa I is inhibited by 5′-methylcytosine at the sites indicated (*) in the recognition sequence.
Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:
ABLMH
100%10-25%50-75%50-75%0-10%
Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained target DNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl2, 200 μM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles. Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 10%. When supplemented with GC-RICH Solution activity is increased to >100%.
Incubation temperature
+30°C
Unit definition
One unit is the enzyme activity that completely cleaves 1 μg ? × Hind III fragments in 1 hour at +30°C in a total volume of 25 μl SuRE/Cut Buffer A.
Heat inactivation
The enzyme can be heat inactivated by incubating it for 15 minutes at +65°C.
Number of cleavage sites on different DNAs
λAd2SV40φ X174M13mp7M13mp18pBR322pBR328pUC18
112 1000000
Ligation and recutting assay
Apa I fragments obtained by complete digestion of 1 μg λ × Hind III fragments are ligated with 1 U T4 DNA Ligase in a volume of 10 μl by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >95% recovery of 1 μg λ × Hind III fragments.
Subsequent re-cutting with Apa I yields >95% of the typical pattern of λ × Hind III× Apa I fragments.
SPH I Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| Career Henan Chemica Co | +86-0371-86658258 15093356674; | laboratory@coreychem.com | China | 30255 | 58 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 821-50328103-801 18930552037 | 3bsc@sina.com | China | 15848 | 69 |
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
| Shanghai Kanglang Biotechnology Co., Ltd. | 021-61998208 18217752821 | sales@klbio.cn | China | 9617 | 58 |
| United States Biological | -- | sales@advtechind.com | United States | 6106 | 58 |
| Supplier | Advantage |
|---|---|
| Creative Enzymes | 58 |
| Career Henan Chemica Co | 58 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 69 |
| Sigma-Aldrich | 80 |
| Shanghai Kanglang Biotechnology Co., Ltd. | 58 |
| United States Biological | 58 |
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