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N-Hydroxysuccinimide

N-Hydroxysuccinimide Structure
N-Hydroxysuccinimide structure
CAS No.
6066-82-6
Chemical Name:
N-Hydroxysuccinimide
Synonyms
NHS;HOSU;1-HYDROXY-PYRROLIDINE-2,5-DIONE;Hydroxysuccinimide;HONSU;2,5-PYRROLIDINEDIONE, 1-HYDROXY-;N-HYDROXYSUCCINIMDE;N-HYDROXYSUCCINAMIDE;1-Hydroxysuccinimide;1-HYDROXY-2,5-PYRROLIDINEDIONE
CBNumber:
CB0391283
Molecular Formula:
C4H5NO3
Molecular Weight:
115.09
MDL Number:
MFCD00005516
MOL File:
6066-82-6.mol
MSDS File:
SDS
Last updated:2026-03-02 16:41:49
Product description Number Pack Size Price
HOSu Novabiochem? 8.51056 25g $34.1
HOSu Novabiochem? 8.51056 100g $82.8
HOSu Novabiochem® 8.51056 1 kg $384
HOSu Novabiochem® 8.51056 1 kg $384
N-Hydroxysuccinimide for synthesis 8.04518 25g $47.6
More product size
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N-Hydroxysuccinimide Properties

Melting point 95-98 °C(lit.)
Boiling point 215.33°C (rough estimate)
Density 1.4769 (rough estimate)
vapor pressure 0Pa at 25℃
refractive index 1.4080 (estimate)
storage temp. Store at +2°C to +8°C.
solubility DMSO (Soluble), Methanol (Slightly)
pka 7.81±0.20(Predicted)
form Liquid or Solid
color Clear yellow-brown to brown
PH 5-7 (50g/l, H2O, 20℃)
Water Solubility SOLUBLE
Sensitive Hygroscopic
BRN 113913
Stability Stable. Incompatible with strong oxidizing agents, acid chlorides, acid anhydrides, strong bases. Protect from moisture.
Cosmetics Ingredients Functions SKIN CONDITIONING
InChI 1S/C4H5NO3/c6-3-1-2-4(7)5(3)8/h8H,1-2H2
InChIKey NQTADLQHYWFPDB-UHFFFAOYSA-N
SMILES ON1C(=O)CCC1=O
LogP -2.01
CAS DataBase Reference 6066-82-6(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII MJE3791M4T
NIST Chemistry Reference N-hydroxylsuccinimide(6066-82-6)
EPA Substance Registry System 2,5-Pyrrolidinedione, 1-hydroxy- (6066-82-6)
UNSPSC Code 12352115
NACRES NA.22

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS05
Signal word  Danger
Hazard statements  H315-H318
Precautionary statements  P264-P280-P302+P352-P305+P351+P338-P332+P313-P362+P364
PPE Eyeshields, Gloves, type N95 (US)
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  22-24/25-26-36
WGK Germany  3
Hazard Note  Irritant
TSCA  TSCA listed
HS Code  29251995
Storage Class 11 - Combustible Solids
Hazard Classifications Eye Dam. 1
Skin Irrit. 2
NFPA 704
1
2 1

N-Hydroxysuccinimide price More Price(79)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 8.51056 HOSu Novabiochem? 6066-82-6 25g $34.1 2025-07-31 Buy
Sigma-Aldrich 8.51056 HOSu Novabiochem? 6066-82-6 100g $82.8 2025-07-31 Buy
Sigma-Aldrich 8.51056 HOSu Novabiochem® 6066-82-6 1 kg $384 2025-07-31 Buy
Sigma-Aldrich 8.51056 HOSu Novabiochem® 6066-82-6 1 kg $384 2025-07-31 Buy
Sigma-Aldrich 8.04518 N-Hydroxysuccinimide for synthesis 6066-82-6 25g $47.6 2025-07-31 Buy
Product number Packaging Price Buy
8.51056 25g $34.1 Buy
8.51056 100g $82.8 Buy
8.51056 1 kg $384 Buy
8.51056 1 kg $384 Buy
8.04518 25g $47.6 Buy

N-Hydroxysuccinimide Chemical Properties,Uses,Production

Description

N-hydroxysuccinimide is a synthetic ingredient used in cosmetics as an ester to soften and condition skin. It's also a reagent, a substance used to trigger a reaction that leads to a new substance, such as peptides and polymers.
N-Hydroxysuccinimide is used in cosmetics and beauty products as an ester often seen in eye creams. N-hydroxysuccinimide activates the elimination of blood originated pigments responsible for dark color and inflammation that causes under eye circles. "Infra-orbital shadows are due to the accumulation of hemoglobin and its coloured degradation products - biliverdin, bilirubin and iron - in the dermis and epidermis ... N-hydroxysuccinimide renders the iron soluble for natural elimination" (source).

Chemical Properties

White to pale yellow

Uses

N-hydroxysuccinimide (NHS) is often used to assist carbodiimide-mediated peptide coupling by forming an active ester intermediate via condensation of the surface carboxyl group and NHS. The NHS-reactive ester intermediate is susceptible to nucleophilic attack by primary amines and results in the formation of stable amide bonds between the biomaterial surface and the N-terminus of the peptide.

Uses

Reagent for preparation of active esters of amino acids.

Uses

N-Hydroxysuccinimide is used for improved amidations in the carbodiimide method. It is also used to activate a carboxyl group and reacts with amine to form amide. It is involved in the preparation of N-hydroxymaleimide-styrene copolymer. Further, it finds use in analytical chemistry. As an additive, it is used in the carbodiimide method for improved amidations and peptide couplings.

Application

N-Hydroxysuccinimide has been used in the synthesis of intermediates such as:
N-succinimidyl 3-(di-tert-butylfluorosilyl)benzoate
4-[2,2-bis[(p-tolylsulfonyl)-methyl] acetyl]benzoic acid-NHS ester
N-succinimidyl 3-iodobenzoate
It has also been used in a protocol for the surface modification of microchannels of a microfluidic-integrated surface plasmon resonance (SPR) platform for the detection and quantification of bacterial pathogens.
Additive used in the carbodiimide method for improved amidations and peptide couplings.
N-Hydroxysuccinimide can be used in the synthesis of N-hydroxysuccinimide ester via dehydration reaction in the presence of dicyclohexylcarbodiimide in carboxylic acid.

reaction suitability

reaction type: Coupling Reactions

Synthesis

N-Hydroxysuccinimide can be synthesized by heating succinic anhydride with hydroxylamine or hydroxylamine hydrochloride.

Purification Methods

Recrystallise the imide from EtOH/ethyl acetate [Manesis & Goodmen J Org Chem 52 5331 1987]. [Beilstein 21/9 V 498.]

N-Hydroxysuccinimide synthesis

53053-08-0
6066-82-6
Synthesis of N-Hydroxysuccinimide from N-HYDROXYSUCCINIMIDYL-4-AZIDOBENZOATE

N-Hydroxysuccinimide Preparation Products And Raw materials

Raw materials

Succinic anhydride Hydroxylamine hydrochloride NSC81536 estradiol-3-sulfate 1-Pyrrolidinecarboxylic acid, 2,5-dioxo-, 1,1-dimethylethyl ester
1-Hydroxypiperidine-2,6-dione N-HYDROXYSUCCINIMIDYL-4-AZIDOBENZOATE N,N'-Disuccinimidyl carbonate N-(3-aminopropyl)benzamide BZ-OSU
Girard's Reagent T N-Succinimidyl 3-maleimidopropionate 2-METHYLBUTYLAMINE

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Preparation Products

Enalapril Aspoxicillin Imidapril N-ACRYLOXYSUCCINIMIDE GUSPERIMUS
Arbekacin Cy5

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N-Hydroxysuccinimide Suppliers

Global( 1018)Suppliers
Supplier Tel Email Country ProdList Advantage
PRISUN PHARMATECH CO.,LTD
+86-021-54326183-8001 +8618917565227 prisun@prisunpharma.com China 215 58
Chemtour Biotech Co., Ltd
+8617327281506 market@chemtour.com China 1521 58
Hebei Chuanghai Biotechnology Co., Ltd 		+8615350571055 Sibel@chuanghaibio.com China 8752 58
Hebei Yanxi Chemical Co., Ltd. 		+8618531123677 faithe@yan-xi.com China 5853 58
Hebei Chuanghai Biotechnology Co,.LTD 		+86-86-13131129325 +8613131129325 sales1@chuanghaibio.com China 5240 58
Hebei Chuanghai Biotechnology Co., Ltd 		+8615531151365 mina@chuanghaibio.com China 18126 58
airuikechemical co., ltd. 		+86-18353166132 sales02@airuikechemical.com China 983 58
Hebei Zhuanglai Chemical Trading Co.,Ltd 		+8613343047651 admin@zlchemi.com China 3692 58
Rongchuang International 		+86-13175533356 +86-13175533356 mihui41770@gmail.com China 245 58
Capot Chemical Co.,Ltd. 		+86-（0）57185586718; +8613336195806 sales@capot.com China 29735 60
Supplier Advantage
PRISUN PHARMATECH CO.,LTD
58
Chemtour Biotech Co., Ltd
58
Hebei Chuanghai Biotechnology Co., Ltd 		58
Hebei Yanxi Chemical Co., Ltd. 		58
Hebei Chuanghai Biotechnology Co,.LTD 		58
Hebei Chuanghai Biotechnology Co., Ltd 		58
airuikechemical co., ltd. 		58
Hebei Zhuanglai Chemical Trading Co.,Ltd 		58
Rongchuang International 		58
Capot Chemical Co.,Ltd. 		60

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Related Qustion

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Q:What is N-Hydroxysuccinimide?Zora - Jan 15，2026
A:N-hydroxysuccinimide (NHS) is defined as a highly reactive compound that is used as a reagent for preparing active esters in peptide synthesis. It can be synthesized by heating succinic anhydride with hydroxylamine or hydroxylamine hydrochloride.

View Lastest Price from N-Hydroxysuccinimide manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
N-Hydroxysuccinimide pictures 2026-03-09 N-Hydroxysuccinimide
6066-82-6
 0.99 RongNa Biotechnology Co.,Ltd
N-Hydroxysuccinimide pictures 2026-03-09 N-Hydroxysuccinimide
6066-82-6
 US $0.00 / g 1g 99% 9999 Rongchuang International
N-Hydroxysuccinimide pictures 2026-03-09 N-Hydroxysuccinimide
6066-82-6
 US $0.00 / Kg/Drum 1KG 99% 500kg Jinan Finer Chemical Co., Ltd

N-Hydroxysuccinimide Spectrum

N-Hydroxysuccinimide(6066-82-6)1HNMRN-Hydroxysuccinimide(6066-82-6)IR1N-Hydroxysuccinimide(6066-82-6)Raman

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N-HYDROXY-2,5-PYRROLIDINEDIONE N-HYDROXYSUCCINIMIDE N-HYDROXYSUCCINIMIDE POLYMER-BOUND NHS N-HYDROXYSUCCINIMIDE, NHS OR HONSU HOSu~1-Hydroxy-2,5-pyrrolidinedione N-Hydroxysuccinimide (HOSu) N-HYDROXYSUCCINIMIDE, 99+% N-HYDROXYSUCCINIMIDE, 98+% Hosu,Nhs(N-Hydroxysuccinimide) 9,10-Bis(Phenthynyl)Anthracene HOSU (N-Hydroxysuccinimide) N-HYDOXYSUCCINIMIDE, NHS HOSU (N-HYDROXYSUCCINIMIDE,NHS) N-Hydroxysuccinimide >97% N-HYDROXYSUCCINIMIDE pure 1-Hydroxy-2,5-pyrrolidinedione, HOSu N-Hydroxysuccinic acid imide Succinimidyl alcohol N-Hydroxysuccinimide [Coupling Reagent for Peptide] O-Hydroxysucc Inimide N-Hydroxysuccinimide,1-Hydroxy-2,5-pyrrolidinedione, HOSu, NHS N-Hydroxysuccinimide≥ 99% (Assay) HOSu Novabiochem 1-hydroxy-5-pyrrolidinedione 5-Pyrrolidinedione,1-hydroxy-2 Succinimide, N-hydroxy- AKOS 91864 4-HYDROXY-2,5-PYRROLIDINEDIONE N-HydroxysucciniMide, 98+% 100GR N-HydroxysucciniMide, 98+% 250GR N-HydroxysucciniMide, 98+% 25GR N-Hydroxy-2,5-dioxopyrrolidine N-HYDROXYSUCCINIMIDE FOR SYNTHESIS 1-Hydroxy-2,5-pyrrolidione 1-Hydroxypyrrolidine-2,5-dione, 2,5-Dioxo-1-hydroxypyrrolidine N-HydroxysucciniMide 98% N-HydroxysucciniMide puruM, >=97.0% (T) N - butyl hydroxy diiMide N-Hydroxysuccinimide N- hydroxyl Ji Dingya diamide 1-Hydroxy-2,5-pyrrolidinedione, NHS, N-Hydroxybutanimide, N-гидроксибутанимид N-Hydroxysuccinimide> N-Hydroxysuccinimide [Coupling Reagent for Peptide]> NHS N-Hydroxysuccinimide,98% HOSU 25 G HOSU/NHS N-Hydroxysuccinimide USP/EP/BP NHS N-HYDROXYSUCCINIMDE N-HYDROXYSUCCINAMIDE 1-Hydroxysuccinimide Hydroxysuccinimide 1-HYDROXY-PYRROLIDINE-2,5-DIONE HONSU HOSU 1-HYDROXY-2,5-PYRROLIDINEDIONE 2,5-PYRROLIDINEDIONE, 1-HYDROXY-