KAPA LongRange

Uses

KAPA LongRange has been used in:

• Amplification of long targets and/or low amounts
• Standard short- and mid-range PCR
• T-A cloning
• Targeted sample enrichment for next-generation DNA sequencing
• polymerase chain reaction (PCR)

General Description

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.

Biochem/physiol Actions

Both KAPA Taq HotStart? DNA polymerase and a modified archaeal (Type B) DNA polymerase enzyme possess 5′→3′ polymerase activity, but only KAPA Taq HotStart DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA LongRange HotStart is 2–4 fold higher than that of Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases such as KAPA HiFi. Reaction products generated with KAPA LongRange HotStart are polyA-tailed and can be cloned into TA-cloning vectors.