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Hippuric acid

Physical and Chemical Properties Hippuric acid excretion test Uses production method
Hippuric acid
Hippuric acid
Chemical Name:
Hippuric acid
CPD-425;hippuric;NSC 9982;BZ-GLY-OH;hippurate;BZO-GLY-OH;AKOS B029712;Hippursaeure;HIPURIC ACID;acidoippurico
Molecular Formula:
Formula Weight:
MOL File:

Hippuric acid Properties

Melting point:
187-191 °C(lit.)
Boiling point:
311.69°C (rough estimate)
1,371 g/cm3
refractive index 
1.5600 (estimate)
storage temp. 
Store at RT.
Crystalline Powder
3.62(at 25℃)
White to almost white
2.5-3.5 (10g/l, H2O, 20℃)(slurry)
Water Solubility 
Soluble in water.
Stable. Incompatible with oxidizing agents.
CAS DataBase Reference
495-69-2(CAS DataBase Reference)
  • Risk and Safety Statements
  • Hazard and Precautionary Statements (GHS)
Hazard Codes  Xn,Xi
Risk Statements  22-37/38-41-36/37/38
Safety Statements  26-39-36/37-22
WGK Germany  3
RTECS  MR8150000
HazardClass  IRRITANT
HS Code  29242990
Signal word: Danger
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H302 Harmful if swallowed Acute toxicity,oral Category 4 Warning P264, P270, P301+P312, P330, P501
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning P264, P280, P302+P352, P321,P332+P313, P362
H318 Causes serious eye damage Serious eye damage/eye irritation Category 1 Danger P280, P305+P351+P338, P310
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning P264, P280, P305+P351+P338,P337+P313P
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning
Precautionary statements:
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P264 Wash hands thoroughly after handling.
P264 Wash skin thouroughly after handling.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P304+P340 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P405 Store locked up.

Hippuric acid price More Price(11)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 112003 Hippuric acid 98% 495-69-2 5g $35.6 2018-11-13 Buy
Sigma-Aldrich 112003 Hippuric acid 98% 495-69-2 100g $37.4 2018-11-13 Buy
TCI Chemical H0143 Hippuric Acid >98.0%(T) 495-69-2 25g $14 2018-11-22 Buy
TCI Chemical H0143 Hippuric Acid >98.0%(T) 495-69-2 100g $28 2018-11-22 Buy
Alfa Aesar A12690 Hippuric acid, 98% 495-69-2 100g $25.3 2018-11-13 Buy

Hippuric acid Chemical Properties,Uses,Production

Physical and Chemical Properties

Hippuric acid is also known as "Benzoylaminoethanoic acid",its molecular formula is C9H9NO3. The molecular weight is 179.18. As colorless crystals. Melting point 187~188 ℃. It's soluble in cold water, chloroform, ether, cold ethanol, slightly soluble in amyl alcohol, soluble in hot water, hot ethanol, sodium phosphate solution, almost insoluble in benzene, carbon disulfide, petroleum ether. Naturally it occurs in herbivores (such as horses) of urine, it is also present in small amounts in human urine,it is easily hydrolyzed to benzoic acid and glycine. Hippuric acid can reduce urine pH within a certain range,which makes the bacteria living environment change, and then plays a synergistic bactericidal effect. Urinary hippuric acid is measured using pyridine-benzene chloride colorimetry. The principle is that hippuric acid reacts with benzene sulfonyl chloride in pyridine solution, and the product in ethanol solution is yellow compound, according to the color depth, quantitative analysis is processed by colorimetry. Normal urine hippuric acid content varies widely because of different varieties of diet and intake (from 0.3 to 2.5 grams per day), and there are individual differences. After Toluene goes into the body, through metabolic transformation, most of it becomes to hippuric acid and is excreted through urine. Determination of horse urine  hippuric acid content can be used as  exposure indicators to reflect the body's absorption of toluene, but it can not serve as indicators of the diagnosis.
Hippuric acid can be made by reaction of benzoyl chloride with glycine in sodium hydroxide solution . It can be used for biochemical reagents and organic synthesis. Hippuric acid ammonium salt is crystal, soluble in water, soluble in ethanol; potassium salt is crystal, containing single molecule of crystal water, soluble in water, soluble in ethanol.
Properties, uses, methods of synthesis of hippuric acid Information compiled by Andy of ChemicalBook (2016-11-19).

Hippuric acid excretion test

The use of the principles of sodium benzoate going into the human body and  binding  glycine  in the liver to be non-toxic hippuric acid and being excreted with the urine,can be used to exam  liver detoxification function, which is called hippuric acid excretion test. The kidneys can not only excrete hippuric acid, it can also synthesize a small amount of  hippuric acid .It is only in normal renal function that hippuric acid test can truly reflect the liver's detoxification function using hippuric acid excretion test . A little after the subjects take breakfast, oral 6g sodium benzoate is taken. 4h after taking the drug, collect the urine, measure the urine excretion, the normal amount ofhippuric acid after 4h discharge should be> 3.0g. To avoid nausea and vomiting induced often by oral sodium benzoate  , intravenous injection 1.77g sodium benzoate can be slowly done  ,urine is collected 1h after injection, discharge amount is measured  , the  normal discharge amount should be 1h> 0.7g. When there is liver parenchymal disease, such as hepatitis, cirrhosis and liver necrosis, the liver synthesis of hippuric acid dysfunctions, which decreases production of hippuric acid  . Obstructive jaundice causes small changes. Reduced renal blood flow and renal excretion dysfunction also lead to the reducing of the amount of hippuric acid discharge . Some fruits contain benzoic acid  such as apples, bananas, etc., they should be banned when testing. Hippuric acid test has many factors and sodium benzoate can cause adverse reactions in patients, and the valuation of liver detoxification function is less precise, it has been less clinical.
Reference: China Medical Encyclopedia twenty-seven DIAGNOSIS  [author:Sun Rongwu]


The product is used for medicine, dyes intermediates and it is used for the production of fluorescent yellow H8GL, disperse fluorescent FFL and so on.

production method

Benzoyl chloride reacts with the amino acid in sodium hydroxide solution, amido sodium benzene is obtained, then it is acidized with hydrochloric acid. The amino acid is dissolved in sodium hydroxide solution, at the same time drop  benzoyl chloride and sodium hydroxide solution  below 30 ℃  , the reaction solution is always alkaline. After finishing adding, stir for 30min. Then add  hydrochloric acid to pH 2, filter  crude, use water to recrystallize , hippuric acid is obtained. Consumption of raw materials fixed: amino acid (90%) 610kg/t, benzoyl chloride (60%) 1520kg/t, caustic soda (30%) 1960kg/t, hydrochloric acid (30%) 900kg/t.

Chemical Properties

White crystalline powder


N-Benzoylglycine also known as Hippuric Acid is the glycine conjugate of benzoic acid commonly found in ruminant urine. It is synthesized in the liver and its production is greatly increased following consuption of benzoic acid. In itself it does not have a direct biological function, however p-hydroxy-hippurica acid can be used as an inhibitor of Ca2+ ATPase.


N-Benzoylglycine also known as Hippuric Acid is the glycine conjugate of benzoic acid commonly found in ruminant urine. Hippuric acid is synthesized in the liver and its production is greatly increase d following consumption of benzoic acid. In itself it does not have a direct biological function, however p-hydroxy-hippurica acid can be used as an inhibitor of Ca2+ ATPase.


ChEBI: An N-acylglycine in which the acyl group is specified as benzoyl.

Purification Methods

Crystallise the acid from boiling H2O. Dry it over P2O5. Also purify it by dissolving 135-140g in 2L of boiling H2O, filtering through a steam-heated funnel and allowing to crystallise at ~20o (yield 115-122g first crop, m 186-187o). [Ingersoll & Babcock Org Synth Coll Vol II 328 1943, Beilstein 9 225, I 100.]

Hippuric acid Preparation Products And Raw materials

Raw materials

Preparation Products

Hippuric acid Suppliers

Global( 214)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Capot Chemical Co.,Ltd.
+86 (0)571-855 867 18
+86 (0)571-858 647 95 China 19953 60
Mainchem Co., Ltd.
+86-0592-6210733 CHINA 32651 55
Anhui Royal Chemical Co., Ltd.
+86-025-86736275 CHINA 477 55
Hubei Jusheng Technology Co.,Ltd.
86-155-27864001 CHINA 18004 58
Chemwill Asia Co.,Ltd.
86-21-51861608;;; CHINA 10967 58
career henan chemical co
+86-371-86658258 CHINA 19990 58
Shanghai Boyle Chemical Co., Ltd. Mr Qiu:021-50182298(Demestic market) Miss Xu:021-50180596(Abroad market)
+86-21-57758967 China 2220 55
J & K SCIENTIFIC LTD. 400-666-7788 +86-10-82848833
+86-10-82849933; China 96815 76
Meryer (Shanghai) Chemical Technology Co., Ltd. +86-(0)21-61259100(Shanghai) +86-(0)755-86170099(ShenZhen) +86-(0)10-62670440(Beijing)
+86-(0)21-61259102(Shanghai) +86-(0)755-86170066(ShenZhen) +86-(0)10-88580358(Beijing) China 40395 62
3B Pharmachem (Wuhan) International Co.,Ltd. 86-21-50328103 * 801、802、803、804 Mobile:18930552037
86-21-50328109 China 15926 69

View Lastest Price from Hippuric acid manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2018-07-31 Hippuric acid
US $150.00 / KG 1KG 99% 1000kg career henan chemical co

Hippuric acid Spectrum

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