2',7'-Dichlorofluorescein
- CAS No.
- 76-54-0
- Chemical Name:
- 2',7'-Dichlorofluorescein
- Synonyms
- 2,7-DICHLOROFLUORESCEIN;DICHLOROFLUORESCEIN;FLUORESCEIN 27;Fluorescein 548;3,6-DICHLOROFLUORAN;2,7-Dichorofluorescin;DICHLOROFLUORESCEIN TS;FLUORESCEIN DICHLORIDE;dcorange25(fluorescein;DICHLORO (R) FLUORESCEIN
- CBNumber:
- CB0690319
- Molecular Formula:
- C20H10Cl2O5
- Molecular Weight:
- 401.2
- MDL Number:
- MFCD00005047
- MOL File:
- 76-54-0.mol
- MSDS File:
- SDS
Melting point | 280 °C (dec.)(lit.) |
---|---|
Boiling point | 514.77°C (rough estimate) |
Density | 0.79 g/mL at 20 °C |
refractive index | 1.5300 (estimate) |
Flash point | 12°C (53.6°F) |
storage temp. | room temp |
solubility | alcohol: passes test |
pka | 4.46(at 25℃) |
form | crystalline |
color | orange to red-brown, powder |
PH Range | 4.0 (colorless) - 6.6 (green) |
Water Solubility | Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water. |
λmax | 509nm |
BRN | 58009 |
Stability | Stable. Incompatible with strong oxidizing agents. |
Major Application | Electroluminescent devices, sol-gel silica films, integrated circuits, photoresists, lithographic printing plates, recording material, LED devices, photoconductors, Solid-state dye laser, lithographic printing plates, inks, Fluorescent pH sensors, emissive probes, chemotherapeutic treatment of disease, phototherapeutic treatment of disease, photodynamic treatment of disease |
InChIKey | NZVMJBGGAQSNLI-UHFFFAOYSA-N |
CAS DataBase Reference | 76-54-0(CAS DataBase Reference) |
FDA UNII | 56NQM5UZT1 |
EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy- (76-54-0) |
SAFETY
Risk and Safety Statements
Symbol(GHS) | GHS07 |
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Signal word | Warning | |||||||||
Hazard statements | H315-H319 | |||||||||
Precautionary statements | P280i-P305+P351+P338-P337+P313-P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313 | |||||||||
Hazard Codes | Xi,F | |||||||||
Risk Statements | 36/37/38-36-67-11 | |||||||||
Safety Statements | 26-36-16 | |||||||||
RIDADR | UN 1219 3/PG 2 | |||||||||
WGK Germany | 3 | |||||||||
RTECS | NT8050000 | |||||||||
TSCA | Yes | |||||||||
HS Code | 3204 19 00 | |||||||||
NFPA 704 |
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2',7'-Dichlorofluorescein price More Price(41)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich | 35848 | 2′,7′-Dichlorofluorescein BioReagent, suitable for fluorescence, ≥90% (T) | 76-54-0 | 5g | $96.4 | 2024-03-01 | Buy |
Sigma-Aldrich | 35848 | 2′,7′-Dichlorofluorescein BioReagent, suitable for fluorescence, ≥90% (T) | 76-54-0 | 25g | $491 | 2024-03-01 | Buy |
Sigma-Aldrich | 1.09676 | 2′,7′-Dichlorofluorescein indicator ACS,Reag. Ph Eur | 76-54-0 | 5G | $355 | 2024-03-01 | Buy |
TCI Chemical | D0371 | 2',7'-Dichlorofluorescein [for Fluorescent indicator] >95.0%(HPLC) | 76-54-0 | 1g | $24 | 2024-03-01 | Buy |
TCI Chemical | D0371 | 2',7'-Dichlorofluorescein [for Fluorescent indicator] >95.0%(HPLC) | 76-54-0 | 25g | $149 | 2024-03-01 | Buy |
2',7'-Dichlorofluorescein Chemical Properties,Uses,Production
Note
Hans et al. report that 2',7'-Dichlorofluorescein (DCF) finds legitimate experimental use in the generation of standard curves used to calibrate DCFH2-DA experimental results, post-chromatography estimation of carbohydrates and in indirect fluorimetric detection in open tubular capillary chromatography (OTC-HPLC). However, DCF is inappropriate for demonstrating ROS as it is oxidized to a non-fluorescent compound in the presence of hydroxyl radicles[1].
Chemical Properties
red to yellow solid
Uses
2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
A new fluorescent sensor for Cd2+ and its application in living cells imaging. Tetrahedron Lett. 2015, 56 (11), 1322-1327.
Colorimetric and fluorogenic signaling of fluoride ions by thiophosphinated dichlorofluorescein. Dyes Pigm. 2015, 112, 170-175.
Uses
Widely used probe, e.g. for determination of carbohydrates by fluorescence densitometry after TLC and determination of H2O2 by chemiluminescence. Fluorescent additives used in indirect fluorimetric detection in OTC-HPLC.
Uses
2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
Definition
2',7'-dichlorofluorescein is a member of 2-benzofurans. It has a role as a fluorochrome. It derives from a fluorescin.
Application
2',7'-dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.
Biological Activity
2',7'-dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).
Biochem/physiol Actions
2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).
References
[1] Chander Hans. “2’,7’-Dichlorofluorescein (DCF) or 2’,7’-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes.” Biomedicine & Pharmacotherapy (2021): 111512.
2',7'-Dichlorofluorescein Preparation Products And Raw materials
Raw materials
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Preparation Products
Supplier | Tel | Country | ProdList | Advantage | |
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Hongxiong | +undefined18915499068 | Suzhouhongxiong@163.com | China | 120 | 58 |
Hebei Mojin Biotechnology Co., Ltd | +86 13288715578 +8613288715578 | sales@hbmojin.com | China | 12446 | 58 |
Henan Fengda Chemical Co., Ltd | +86-371-86557731 +86-13613820652 | info@fdachem.com | China | 20293 | 58 |
Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 | info@dakenam.com | China | 18628 | 58 |
career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29888 | 58 |
Changzhou Ansciep Chemical Co., Ltd. | +86 519 86305871 | sales@ansciepchem.com | CHINA | 4242 | 58 |
Xi'an Kono chem co., Ltd., | 029-86107037 13289246953 | info@konochemical.com | China | 2995 | 58 |
Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 | sales@chemdad.com | China | 39916 | 58 |
Career Henan Chemica Co | +86-0371-86658258 +8613203830695 | laboratory@coreychem.com | China | 30250 | 58 |
Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +8618949823763 | sales@tnjchem.com | China | 34571 | 58 |
Related articles
- The uses of 2',7'-Dichlorofluorescein
- DCF is used directly, there is a decline in fluorescence in the presence of ROS. Hence, DCFH2-DA (not DCF) should be used to i....
- Jan 18,2024
View Lastest Price from 2',7'-Dichlorofluorescein manufacturers
Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
---|---|---|---|---|---|---|---|---|
2024-04-16 | dichlorofluorescein
76-54-0
|
US $5.00-2.00 / kg | 1kg | 99% | g-kg-tons, free sample is available | Henan Fengda Chemical Co., Ltd | ||
2023-10-16 | dichlorofluorescein
76-54-0
|
US $0.00 / KG | 1KG | 99% | 50000KG/month | Hebei Mojin Biotechnology Co., Ltd | ||
2023-09-06 | dichlorofluorescein
76-54-0
|
US $0.00 / KG | 1KG | 99% | 500000kg | Hebei Guanlang Biotechnology Co., Ltd. |
- dichlorofluorescein
76-54-0
- US $5.00-2.00 / kg
- 99%
- Henan Fengda Chemical Co., Ltd
- dichlorofluorescein
76-54-0
- US $0.00 / KG
- 99%
- Hebei Mojin Biotechnology Co., Ltd
- dichlorofluorescein
76-54-0
- US $0.00 / KG
- 99%
- Hebei Guanlang Biotechnology Co., Ltd.
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