ChemicalBook >> CAS DataBase List >>ACRIDINE ORANGE

ACRIDINE ORANGE

CAS No.
65-61-2
Chemical Name:
ACRIDINE ORANGE
Synonyms
AO;AOX1;Lysotraker;CI NO 46005;BASIC ORANGE;-Cellstain-Ao;Euchrysine 3r;EUCHRYSINE 3RX;acridineoranger;RHODULINE ORANGE
CBNumber:
CB0747468
Molecular Formula:
C17H20ClN3
Molecular Weight:
301.82
MDL Number:
MFCD00012660
MOL File:
65-61-2.mol
Last updated:2024-03-26 18:04:44

ACRIDINE ORANGE Properties

Melting point 284-287 °C(lit.)
Density 1.001 g/mL at 20 °C
refractive index n20/D 1.338
storage temp. -20°C
solubility Soluble in water, ethanol, dimethyl sulfoxide
form Powder
pka -3.2, 10.5(at 25℃)
Colour Index 46005
color orange to red
λmax 500 nm
BRN 3734978
Biological Applications Detecting cancer cells,spores,human papilloma virus (HPV),stress biomarkers; treating amyloid associated diseases,lupus,pathogen infections; apoptosis assays; cytotoxicity assays
Major Application Adhesives; aluminophosphate crystalline materials; detecting clay particles; display device; evaluating fiber surface characteristics; glass matrixes; imaging material; inks; lasers; recording materials; photoresists; textiles; thin films; tracers for hydrology; wiring boards
CAS DataBase Reference 65-61-2
FDA UNII YO2175B15I
EPA Substance Registry System C.I. Basic Orange 14 (65-61-2)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS08
Signal word  Warning
Hazard statements  H341
Precautionary statements  P201-P202-P280-P308+P313-P405-P501
Safety Statements  22-24/25
WGK Germany  3
RTECS  AR7601000
HS Code  32041300
Toxicity dnd-mus:ast 20 mmol/L BBACAQ 374,96,74

ACRIDINE ORANGE price More Price(28)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 318337 Acridine Orange hydrochloride hydrate 99% (HPLC) 65-61-2 1g $146 2024-03-01 Buy
Sigma-Aldrich 318337 Acridine Orange hydrochloride hydrate 99% (HPLC) 65-61-2 5g $714 2024-03-01 Buy
Sigma-Aldrich A9231 Acridine Orange solution 65-61-2 10ml $165 2024-03-01 Buy
Sigma-Aldrich 318337 Acridine Orange hydrochloride hydrate 99% (HPLC) 65-61-2 250mg $41.5 2024-03-01 Buy
Sigma-Aldrich A8097 Acridine Orange hydrochloride solution 10mg/mL in H 65-61-2 10ml $106 2024-03-01 Buy
Product number Packaging Price Buy
318337 1g $146 Buy
318337 5g $714 Buy
A9231 10ml $165 Buy
318337 250mg $41.5 Buy
A8097 10ml $106 Buy

ACRIDINE ORANGE Chemical Properties,Uses,Production

Chemical Properties

dark orange to brown powder

Uses

Acridine orange is a dye that interacts with DNA and RNA by intercelation or electrostatic attraction. This cationic dye has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels.

Preparation

4,4 ‘-Bis (N, N-dimethyl) aminodiphenylmethane obtained dinitration of 4,4′ – bis (N, N-dimethyl) amino-2 ‘- dinitro-diphenyl methane reduction, and with an acid of heat, so that the diamino compound is cyclized product is oxidized and then cyclized and converted into zinc chloride double salt.

Biological Activity

acridine orange hydrochloride is a cell and organelle membrane permeable fluorescent dye that targets nucleic acids. acridine orange can either be incorporated into double helical nucleic acids (detected by green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (detected by red fluorescence at 640 nm), which renders it the ability to differently stain dna and rna or single-stranded dna in situ. for unfixed cells, acridine orange staining can still be used as a simple, fast approach of obtaining information on cell cycle status and cell ploidy levels from dna staining (green fluorescence), as well as cell transcriptional activity from rna measurements (red fluorescence), in a flow cytofluorometric system. thus, acridine orange may serve as a useful cytochemical stain for cell-cycle and apoptosis studies.1. mcmaster gk, carmichael gg. analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. proceedings of the national academy of sciences of the united states of america, 1977, 74(11): 4835-4838.2. traganos f, darzynkiewicz z, sharpless t, et al. simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. journal of histochemistry and cytochemistry, 1977, 25(1): 46-56.

Safety Profile

Mutation data reported. Whenheated to decomposition it emits very toxic fumes of HCl,Cl??, and NOx.

Enzyme inhibitor

This cell-permeable dye (FWfree-base = 265.36 g/mol; CAS 65-61-2), also known as N,N,N',N'-tetramethylacridine-3,6-diamine, is an intercalator that binds tightly to nucleic acids. Binding to DNA is characterized by excitation maximum at 502 nm (cyan) and an emission maximum at 525 nm, producing a green fluorescence. (A 120 μM solution of acridine orange will detect 25-50 ng DNA per band in an agarose or polyacrylamide gel.) Binding to RNA is characterized by an excitation maximum at 460 nm and the emission maximum is located at 650 nm, yielding a reddish-orange fluorescence. Acridine orange also penetrates acidic compartments (e.g., lysosomes and phagolysosomes); upon protonation, the dye emits orange light when excited by blue light.

storage

Room temperature

Properties and Applications

bright yellow orange. Soluble in water and ethanol for orange yellow, with green fluorescent. The strong sulfuric acid in nearly colorless, a green fluorescent, diluted for orange. The dye solution with strong sodium hydroxide solution have yellow precipitation. And C.I. Solvent Orange 15 the same chemical structure (free base).

Standard( Silk ) Light Fastness Persperation Fastness Ironing Fastness Soaping
Fading Stain Fading Stain Fading Stain
ISO 1-2 3 1 5 1 3

ACRIDINE ORANGE Preparation Products And Raw materials

Global( 126)Suppliers
Supplier Tel Email Country ProdList Advantage
Henan Fengda Chemical Co., Ltd
+8613613820652 info@fdachem.com China 5085 58
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21700 55
Hubei Jusheng Technology Co.,Ltd.
18871490254 linda@hubeijusheng.com CHINA 28180 58
Zhengzhou Alfa Chemical Co.,Ltd
+8618530059196 sale04@alfachem.cn China 12333 58
TargetMol Chemicals Inc.
+1-781-999-5354 +1-00000000000 marketing@targetmol.com United States 19892 58
GERBU Biotechnik GmbH
+49-62217264160 +49-62217264160 found@gerbu.de Germany 4 58
Nantong HI-FUTURE Biology Co., Ltd.
+undefined18051384581 sales@chemhifuture.com China 3136 58
ZHEJIANG JIUZHOU CHEM CO., LTD
+86-0576225566889 +86-13454675544 admin@jiuzhou-chem.com;jamie@jiuzhou-chem.com;alice@jiuzhou-chem.com China 20000 58
GIHI CHEMICALS CO.,LIMITED
+8618058761490 info@gihichemicals.com China 50001 58
Wuhan Topule Biopharmaceutical Co., Ltd
+8618327326525 masar@topule.com China 8475 58

View Lastest Price from ACRIDINE ORANGE manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
ACRIDINE ORANGE pictures 2024-03-26 ACRIDINE ORANGE
65-61-2
US $5.00-0.20 / KG 1KG 98% g-kg-tons, free sample is available Henan Fengda Chemical Co., Ltd
Acridine orange pictures 2022-08-10 Acridine orange
65-61-2
US $212.00 / g 1g excl. 19% VAT 20T GERBU Biotechnik GmbH
3,6-BIS(DIMETHYLAMINO)ACRIDINE HYDROCHLORIDE ACRIDINE ORANGE (E GURR) ACRIDINE ORANGE HYDROCHLORIDE ACRIDINE ORANGE N BASIC ORANGE BIS(DIMETHYLAMINO) ACRIDINE HYDROCHLORIDE AO CI NO 46005 EUCHRYSINE 3RX rhodulineorangeno N,N,N',N'-tetramethylacridin-3,6-yldiamine hydrochloride ACRIDINE ORANGE SOLUTION ACRIDINE ORANGE HYDROCHLORIDE SOLUTION, ~1% IN WATER ACRIDINE ORANGE FOR MICROSCOPY ACRIDINE ORANGE HYDROCHLORIDE HYDRATE, F OR MICROSCOPY ACRIDINE ORANGE HYDROCHLORIDE HYDRATE 80% -Cellstain-Ao -Cellstain-AoSolution ACRIDING ORANGE HYDROCHLORIDE 3,6-Bis(dimethylamino) acridine, zinc chloride double salt Acridine Orange, hydrochloride hydrate, ca. 80 % dye content, pure AOX1 acridine orange hydrochloride solution 3,6-Bis(dimethylamino)acridine,hydrochloride,hydrate,aqueoussolution ACRIDINE ORANGE N-HYRATE Basic orange 14 (C.I. 46005) Acridine Orange solution hydrochloride Acridine Orange hydrate hydrochloride Acridine Orange, hydrochloride hydrate,pure,ca. 80 % dye content Acridine Orange hydrochloride solution,3,6-Bis(dimethylamino)acridine hydrochloride ACRIDINE ORANGE HYDROCHLORIDE HYDRATE PURUM, FOR MICROSCOPY ((FL., HIST.)) 98% RHODULINE ORANGE 3,6-BIS(DIMETHYLAMINO)ACRIDINE, HCL 3,6-acridinediamine, N,n,n0,n0-tetramethyl-, monohydrochloride Acridine, 3,6-bis(dimethylamino)-, Hydrochloride Acridine, 3,6-bis(dimethylamino)-, Monohydrochloride Euchrysine 3r Sumitomo Acridine Orange No Sumitomo acridine Orange Rk Conc 3,6-Acridinediamine,N,N,N’,N’-tetramethyl-,monohydrochloride 3,6-bis(dimethylamino)-acridinmonohydrochloride 6-acridinediamine,n,n,n’,n’-tetramethyl-monohydrochloride acridineorangeno acridineorangens acridineoranger c.i.basicorange14 6-acridinediamine 6-Bis(dimethylamino)acridine hydrochloride 3 Lysotraker N’-Tetramethyl-3 N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride N3,N3,N6,N6-Tetramethylacridine-3,6-diamine hydrochloride Acridine Orange hydrochloride?, >99% N3,N3,N6,N6-tetramethylacridine-3,6-diamine chloride Cellstain AO Solution (1mg/ml H2O) Acridine Orange Solution [for Cell Staining] C.I.Basic Orange 14:C.I.46005 Acridine orange hydrochloride, hydrate, ≥98% (HPLC)