Phenosafranin
- CAS No.
- 81-93-6
- Chemical Name:
- Phenosafranin
- Synonyms
- nsc9855;CI 50200;CI NO 50200;schultzno.958;PHENOSAFRANIN;phenosofrinine;phenosofranine;Phenosafeanine;safrininbextra;PHENOSAFRANINE
- CBNumber:
- CB1460102
- Molecular Formula:
- C18H15ClN4
- Molecular Weight:
- 322.8
- MDL Number:
- MFCD00036335
- MOL File:
- 81-93-6.mol
| Melting point | 300 °C |
|---|---|
| Boiling point | 478.49°C (rough estimate) |
| Density | 1.1738 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | room temp |
| Colour Index | 50200 |
| Merck | 13,7333 |
| BRN | 3642082 |
| CAS DataBase Reference | 81-93-6 |
| FDA UNII | 47U3T2FC2W |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P264-P271-P280-P302+P352-P305+P351+P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
| RTECS | SG1630000 |
Phenosafranin price More Price(12)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 199648 | Phenosafranin Dye content 80 % | 81-93-6 | 1g | $69.8 | 2024-03-01 | Buy |
| TRC | P318020 | Phenosafranin | 81-93-6 | 50mg | $155 | 2021-12-16 | Buy |
| Usbiological | 241218 | Phenosafranin | 81-93-6 | 1g | $156 | 2021-12-16 | Buy |
| Usbiological | 286776 | Phenosafranin | 81-93-6 | 25mg | $446 | 2021-12-16 | Buy |
| American Custom Chemicals Corporation | CHM0031745 | PHENOSAFRANINE 95.00% | 81-93-6 | 1G | $128.1 | 2021-12-16 | Buy |
Phenosafranin Chemical Properties,Uses,Production
Chemical Properties
green crystalline powder
Uses
Phenosafranin is a bacterial stain used in microscopy, biological stain, and nuclear staining.
Definition
ChEBI: Phenosafranine is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. It has a role as a fluorochrome, a histological dye and a photosensitizing agent. It contains a 3,7-diamino-5-phenylphenazin-5-ium.
Biochem/physiol Actions
Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally.
Staining Procedures
Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min.
Purification Methods
Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.]
Phenosafranin Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Hubei xin bonus chemical co. LTD | 86-13657291602 | linda@hubeijusheng.com | CHINA | 22968 | 58 |
| Henan Alfa Chemical Co., Ltd | +8618339805032 | alfa4@alfachem.cn | China | 12755 | 58 |
| LEAP CHEM CO., LTD. | +86-852-30606658 | market18@leapchem.com | China | 24738 | 58 |
| Nanjing Duly Biotech Co.,Ltd | +undefined18013301590 | melody@njduly.com | China | 2452 | 58 |
| ZHEJIANG JIUZHOU CHEM CO., LTD | +86-0576225566889 +86-13454675544 | admin@jiuzhou-chem.com；jamie@jiuzhou-chem.com；alice@jiuzhou-chem.com | China | 20000 | 58 |
| Aladdin Scientific | +1-833-552-7181 | sales@aladdinsci.com | United States | 57511 | 58 |
| J & K SCIENTIFIC LTD. | 010-82848833 400-666-7788 | jkinfo@jkchemical.com | China | 94838 | 76 |
| BeiJing Hwrk Chemicals Limted | 0757-86329057 18934348241 | sales4.gd@hwrkchemical.com | China | 14067 | 55 |
| Adamas Reagent, Ltd. | 400-6009262 16621234537 | zhangsn@titansci.com | China | 14113 | 59 |
| Chemsky (shanghai) International Co.,Ltd | 021-50135380 | shchemsky@sina.com | China | 15421 | 60 |