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NUCLEI ISOLATION KIT: NUCLEI PURE PREP

NUCLEI ISOLATION KIT: NUCLEI PURE PREP Structure
NUCLEI ISOLATION KIT: NUCLEI PURE PREP structure
CAS No.
Chemical Name:
NUCLEI ISOLATION KIT: NUCLEI PURE PREP
Synonyms
NUCLEI ISOLATION KIT: NUCLEI PURE PREP
CBNumber:
CB1564220
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Request For Quotation

NUCLEI ISOLATION KIT: NUCLEI PURE PREP Properties

storage temp. 2-8°C

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS05
Signal word  Danger
Hazard statements  H318-H412
Precautionary statements  P273-P280-P305+P351+P338
Hazard Codes  Xi
Risk Statements  41-52/53
Safety Statements  26-39-61

NUCLEI ISOLATION KIT: NUCLEI PURE PREP price

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich NUC201 Nuclei Isolation Kit: Nuclei PURE Prep sufficient for 15?nuclei preparations (~1-10×107 cells or 1g of tissue per preparation) 1KIT $630 2024-03-01 Buy
Product number Packaging Price Buy
NUC201 1KIT $630 Buy

NUCLEI ISOLATION KIT: NUCLEI PURE PREP Chemical Properties,Uses,Production

Uses

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Biochem/physiol Actions

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

NUCLEI ISOLATION KIT: NUCLEI PURE PREP Preparation Products And Raw materials

Raw materials

Preparation Products

NUCLEI ISOLATION KIT: NUCLEI PURE PREP Suppliers

Global( 2)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
NUCLEI ISOLATION KIT: NUCLEI PURE PREP