ENTEROKINASE
- CAS No.
- 9014-74-8
- Chemical Name:
- ENTEROKINASE
- Synonyms
- REK;ENTK;PRSS7;TMPRSS15;EC 3.4.21.9;ENTEROKINASE;Enterrokinase;ENTEROPEPTIDASE;Serine protease 7;Peptidase, entero-
- CBNumber:
- CB2204181
- Molecular Formula:
- NULL
- Molecular Weight:
- 0
- MDL Number:
- MFCD00131020
- MOL File:
- Mol file
- MSDS File:
- SDS
| storage temp. | -20°C |
|---|---|
| form | salt-free, lyophilized powder |
| color | white |
| CAS DataBase Reference | 9014-74-8 |
| FDA UNII |
ENTEROPEPTIDASE (PORCINE) (1N6UU71ZTI) ENTEROPEPTIDASE (HUMAN) (228MAT8QUT) ENTEROPEPTIDASE (BOVINE) (7QBB4Y898A) |
| EPA Substance Registry System | Peptidase, entero- (9014-74-8) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS07,GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H315-H319-H334-H335 |
| Precautionary statements | P261-P284-P304+P340-P305+P351+P338-P342+P311 |
| WGK Germany | 3 |
ENTEROKINASE price More Price(8)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | E0885 | Enterokinase from porcine intestine lyophilized powder, ≥100?units/mg protein | 9014-74-8 | 40units | $154.8 | 2024-03-01 | Buy |
| Sigma-Aldrich | E5144 | Enterokinase from bovine intestine powder | 9014-74-8 | 50units | $388 | 2024-03-01 | Buy |
| Sigma-Aldrich | E0885 | Enterokinase from porcine intestine lyophilized powder, ≥100?units/mg protein | 9014-74-8 | 200units | $1050 | 2024-03-01 | Buy |
| Sigma-Aldrich | E0632 | Enterokinase from porcine intestine ≥0.5?units/mg solid | 9014-74-8 | 1500Units | $400 | 2024-03-01 | Buy |
| Sigma-Aldrich | 69066-M | Recombinant Enterokinase | 9014-74-8 | 50u | $234 | 2024-03-01 | Buy |
ENTEROKINASE Chemical Properties,Uses,Production
Uses
Typical conditions for fusion protein cleavage:
Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween? 20
Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix
Incubate reaction mixture at ~25 °C for 16 hours
Definition
An enzyme found in the small intestine, which converts trypsinogen into trypsin.
General Description
Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.
Biochem/physiol Actions
Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.
ENTEROKINASE Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Zibo Hangyu Biotechnology Development Co., Ltd | +86-0533-2185556 +8617865335152 | Mandy@hangyubiotech.com | China | 11013 | 58 |
| Shanghai Zheyan Biotech Co., Ltd. | 18017610038 | zheyansh@163.com | CHINA | 3620 | 58 |
| career henan chemical co | +86-0371-86658258 15093356674; | factory@coreychem.com | China | 29826 | 58 |
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| ANHUI WITOP BIOTECH CO., LTD | +8615255079626 | eric@witopchemical.com | China | 23556 | 58 |
| Wuhan Fortuna Chemical Co., Ltd | +86-27-59207850 +86-13986145403 | info@fortunachem.com | China | 5985 | 58 |
| Henan Alfa Chemical Co., Ltd | +8618339805032 | alfa4@alfachem.cn | China | 12755 | 58 |
| Hebei Duling International Trade Co. LTD | +8618032673083 | sales05@hbduling.cn | China | 15747 | 58 |
| Nanjing Duly Biotech Co.,Ltd | +undefined18013301590 | melody@njduly.com | China | 2452 | 58 |
| LEAPCHEM CO., LTD. | +86-852-30606658 | market18@leapchem.com | China | 43348 | 58 |
View Lastest Price from ENTEROKINASE manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2024-01-02 | ENTEROKINASE
9014-74-8
|
US $50.00 / kg | 10kg | 0.99 | 20tons | Zibo Hangyu Biotechnology Development Co., Ltd | |
 |
2021-09-29 | Enteropeptidase
9014-74-8
|
US $0.00-0.00 / g | 1g | 98.0% | 1kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | |
 |
2020-01-10 | Enterokinase
9014-74-8
|
US $1.00 / KG | 1KG | ≥98% | 20 tons | Career Henan Chemical Co |
-
- ENTEROKINASE
9014-74-8
- US $50.00 / kg
- 0.99
- Zibo Hangyu Biotechnology Development Co., Ltd
-
- Enteropeptidase
9014-74-8
- US $0.00-0.00 / g
- 98.0%
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- Enterokinase
9014-74-8
- US $1.00 / KG
- ≥98%
- Career Henan Chemical Co