ChemicalBook >> CAS DataBase List >>5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid)

CAS No.
69-78-3
Chemical Name:
5,5′-Dithiobis(2-nitrobenzoic acid)
Synonyms
DTNB;Ba-2767;5,5'Ellmanns;5,5&priMe;Ellmsn'sreagent;ELLMANS' REAGENT;ELLMAN'S REAGENT;ellmann's reagent;SAPONIN BIOCHEMICAL
CBNumber:
CB2215444
Molecular Formula:
C14H8N2O8S2
Molecular Weight:
396.35
MDL Number:
MFCD00007140
MOL File:
69-78-3.mol
MSDS File:
SDS
Last updated:2023-12-24 21:24:35

5,5′-Dithiobis(2-nitrobenzoic acid) Properties

Melting point 240-245 °C (dec.)(lit.)
Boiling point 671.9±55.0 °C(Predicted)
Density 1.67
refractive index 1.6900 (estimate)
storage temp. Keep in dark place,Sealed in dry,Room Temperature
pka 1.57±0.25(Predicted)
form Yellow solid
color Pale Yellow to Light Yellow
Water Solubility Soluble in water and ethanol.
Merck 14,3551
BRN 1896821
Stability Stable. Incompatible with strong bases, strong oxidizing agents.
InChIKey KIUMMUBSPKGMOY-UHFFFAOYSA-N
CAS DataBase Reference 69-78-3(CAS DataBase Reference)
FDA UNII 9BZQ3U62JX
EPA Substance Registry System Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313-P261-P280a-P304+P340-P305+P351+P338-P405-P501a
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  26-36
WGK Germany  3
RTECS  DG9650000
10
TSCA  Yes
HS Code  29309070
NFPA 704
1
2 0

5,5′-Dithiobis(2-nitrobenzoic acid) price More Price(59)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich D218200 5,5′-Dithiobis(2-nitrobenzoic acid) ReagentPlus , 99% 69-78-3 1g $32.3 2024-03-01 Buy
Sigma-Aldrich 322123 5,5ʹ-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem Sulfhydryl reagent used to characterize reactive SH groups. 69-78-3 5G $140 2024-03-01 Buy
TCI Chemical D0944 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups] >98.0%(HPLC)(T) 69-78-3 1g $29 2024-03-01 Buy
TCI Chemical D0944 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups] >98.0%(HPLC)(T) 69-78-3 5g $75 2024-03-01 Buy
Alfa Aesar A14331 5,5'-Dithiobis(2-nitrobenzoic acid), 99% 69-78-3 1g $34.9 2024-03-01 Buy
Product number Packaging Price Buy
D218200 1g $32.3 Buy
322123 5G $140 Buy
D0944 1g $29 Buy
D0944 5g $75 Buy
A14331 1g $34.9 Buy

5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties,Uses,Production

Chemical Properties

Crystalline

Uses

Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

Uses

5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.

Uses

5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.

Definition

ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.

General Description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

5,5′-Dithiobis(2-nitrobenzoic acid) Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 371)Suppliers
Supplier Tel Email Country ProdList Advantage
Hebei Mojin Biotechnology Co., Ltd
+8613288715578 sales@hbmojin.com China 12452 58
Hebei Yanxi Chemical Co., Ltd.
+8617531190177 peter@yan-xi.com China 5990 58
Henan Fengda Chemical Co., Ltd
+8613613820652 info@fdachem.com China 5505 58
Shanghai Daken Advanced Materials Co.,Ltd
+86-371-66670886 info@dakenam.com China 14936 58
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21700 55
Hangzhou FandaChem Co.,Ltd.
008657128800458; +8615858145714 fandachem@gmail.com China 9354 55
ATK CHEMICAL COMPANY LIMITED
+undefined-21-51877795 ivan@atkchemical.com China 32480 60
career henan chemical co
+86-0371-86658258 sales@coreychem.com China 29914 58
Yunbio Tech Co.,Ltd.
+86-010-60605551 +86-18046518538 yunbiochem@126.com China 321 58
Hebei Guanlang Biotechnology Co., Ltd.
+86-19930503282 alice@crovellbio.com China 8823 58

Related articles

View Lastest Price from 5,5′-Dithiobis(2-nitrobenzoic acid) manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
5,5′-Dithiobis(2-nitrobenzoic acid) pictures 2023-12-24 5,5′-Dithiobis(2-nitrobenzoic acid)
69-78-3
US $100.00-1.00 / KG 1KG 99% g-kg-tons, free sample is available Henan Fengda Chemical Co., Ltd
5,5′-Dithiobis(2-nitrobenzoic acid) pictures 2023-09-28 5,5′-Dithiobis(2-nitrobenzoic acid)
69-78-3
US $100.00-10.00 / kgkg 1kgkg 0.99 10000 Hebei Yanxi Chemical Co., Ltd.
5,5′-Dithiobis(2-nitrobenzoic acid) pictures 2023-09-06 5,5′-Dithiobis(2-nitrobenzoic acid)
69-78-3
US $0.00-0.00 / KG 1KG 99% 500000kg Hebei Guanlang Biotechnology Co., Ltd.
ellmann's reagent ELLMANS' REAGENT BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE 3-CARBOXY-4-NITROPHENYL DISULFIDE Ellmsn'sreagent Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans Ellmanns 5,5'-Dithobis(2-Nitrobenzoic Acid) 5,5'-DITHIO-BIS(2-NITROBENZOIC ACID) (3, 3'-6) 5.5'-Dithio-Bis-(2-NitrobenzoicAcid)A.R. Dithiobis(2-nitrobenzoic acid) 5,5-Dithiobis(2-nitrobenzoic acid)product (DTNB) Benzoic acid, 3,3-dithiobis6-nitro- 5-[(3-CARBOXY-4-NITROPHENYL)DITHIO]-2-NITROBENZOICACID(5,5-DITHIOBIS(2-NITROBENZOICACID)) 5,5-Dithiobis(2-nitrobenzoic acid) (DTNB) 5,5'-DITHIOBIS(2-NITROBENZOIC ACID) 5,5-DITHIO-BIS(2-NITROBENZOIC ACID) 5-[(3-CARBOXY-4-NITROPHENYL)DITHIO]-2-NITROBENZOIC ACID 6,6'-DINITRO-3,3'-DITHIODIBENZOIC ACID 2,2'-DINITRO-5,5'-DITHIODIBENZOIC ACID dithionitrobenzoicacid (3-Carboxy-4-nitrophenyl)disulphide, DTNB, Ellman's reagent 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups] 5,5-DITHIOBIS(2-NITROBENZOIC ACID) extrapure 5,5'-Dithiobis(2-nitrobenzoic acid) Vetec(TM) reagent grade, 98% SAPONIN BIOCHEMICAL DTNB Ellman's Reagent 2,2’-dinitro-5,5’-dithiodibenzoesaeure 3,3’-dithiobis(6-nitro-benzoicaci 3,3’-dithiobis(6-nitrobenzoicacid) 3,3’-dithiobis[6-nitro-benzoicaci 5,5’-dithiobis(2-nitrobenzoic 3-Carboxy-4-nitrophenyl disulfide, 6,6μ-Dinitro-3,3μ-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellmans Reagent 5,5'-Dithiobis(2-nitrobenzoic acid) ,98% 5,5'-DITHIOBIS(2-NITROBENZOICACID) RESEARCH GRADE 5,5′-Dithiobis(2-nitrobenzoic acid),3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent 3-Carboxy-4-nitrophe 5-(3-Carboxy-4-nitrophenyl)disulfanyl-2-nitrobenzoic acid 5,5'-Dithiobis(2-nitrobenzoic acid) ,99% Ba-2767 Di(3-carboxy-4-nitrophenyl) persulfide Di(3-carboxy-4-nitrophenyl)persulfide Bis(3-carboxy-4-nitrophenyl) Disulphide Bis(3-carboxy-4-nitrophenyl) Disulfide DTNB Ellman's Reagent 5-Dithiobis(2-nitrobenzoic acid) 5,5'-disulfanediylbis(2-nitrobenzoic acid) 5,5&priMe 5,5 'twothiobis(2-Nitrobenzoic Acid 5,5'-Dithiobis(2-nitrobenzoic acid) ReagentPlus(R), 99% ELLMAN'S REAGENT DTNB, 5,5'-Dithiobis-(2-nitrobenzoic acid) Dithiobis(2-nitrobenzoic acid) research grade 5,5-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]> ithionitrobenzoicaci 5,5′-Dithiobis(2-nitrobenzoic acid) ISO 9001:2015 REACH 3-Carboxy-4-nitrophenyl disulfide 69-78-3 5,5-Dithiobis(2-Nitro Benzoic Acid) extrapure (DTNB), 98%