Duolink In Situ Detection Reagents Green

Uses

Duolink^? In Situ Detection Reagents has been used in the proximity ligation assay of:

• vasopressin and gonadotropin-releasing hormone (GnRH) from frozen rat brain sections
• human embryonic kidney 293 cells (HEK)
• pituitary tissues

General Description

Duolink^? In Situ Detection Reagents Green contains all the necessary Duolink In situ reagents to perform the amplification and detection of bound PLA^? probes. The detection probes contain a fluorophore (lex = 495 nm and lem = 527 nm), which may be visualized using the same filter as Cy^?2 or FITC. Experiments conducted using Duolink In situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.