Duolink In Situ Detection Reagents FarRed

CAS No.
Chemical Name:
Duolink In Situ Detection Reagents FarRed
Synonyms
Duolink? In Situ Detection Reagents FarRed
CBNumber:
CB22865029
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file

Duolink In Situ Detection Reagents FarRed Properties

storage temp. -20°C

Duolink In Situ Detection Reagents FarRed price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich DUO92013 Duolink® In Situ Detection Reagents FarRed 30REACTIONS $405 2023-06-20 Buy
Sigma-Aldrich DUO92013 Duolink® In Situ Detection Reagents FarRed 100REACTIONS $714 2023-06-20 Buy
Product number Packaging Price Buy
DUO92013 30REACTIONS $405 Buy
DUO92013 100REACTIONS $714 Buy

Duolink In Situ Detection Reagents FarRed Chemical Properties,Uses,Production

Uses

Duolink?proximity ligation assay(PLA?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink? In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink? PLA Resource Center for information on how to run a Duolink? experiment, applications, troubleshooting, and more.

To perform a complete Duolink? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
FarRed fluorescence detection reagents are often used with Cyanine 5 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink? in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink? projects

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General Description

Duolink? In Situ Detection Reagents Far Red contains all the necessary Duolink In situ reagents to perform the amplification and detection of bound PLA? probes. The detection probes contain a fluorophore (lex = 644nm and lem = 669nm), which may be visualized using the same filter as Cy?5. Experiments conducted using Duolink In Situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.

Duolink In Situ Detection Reagents FarRed Preparation Products And Raw materials

Raw materials

Preparation Products

Duolink In Situ Detection Reagents FarRed Suppliers

Global( 2)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
Duolink? In Situ Detection Reagents FarRed