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Diethyl pyrocarbonate

Chemical Name:
Diethyl pyrocarbonate
DEPC;DIETHYL DICARBONATE;Ethyl pyrocarbonate;DKD;Piref;Baycovin;DEP/DEPC;ethylpyrocarbonate;Diethylpyrokarbonat;iethylpyrocarbonate
Molecular Formula:
Molecular Weight:
MOL File:
MSDS File:

Diethyl pyrocarbonate Properties

Melting point 69℃
Boiling point 93-94 °C18 mm Hg(lit.)
Density 1.12 g/mL at 20 °C
refractive index n20/D 1.398(lit.)
Flash point 157 °F
storage temp. 2-8°C
solubility 95% ethanol: soluble4.5g/10 mL, clear, colorless
form Liquid
color APHA: ≤20
Water Solubility slow decomposition
Sensitive Moisture Sensitive
Merck 14,7998
BRN 637031
Stability Combustible. Unstable - readily decomposes. Incompatible with strong oxidizing agents, strong acids, strong reducing agents, strong bases, ammonia.
CAS DataBase Reference 1609-47-8(CAS DataBase Reference)
FDA 21 CFR 189.140
Substances Added to Food (formerly EAFUS) DIETHYL PYROCARBONATE-- PROHIBITED
EWG's Food Scores 1
NIST Chemistry Reference Diethyl pyrocarbonate(1609-47-8)
EPA Substance Registry System Dicarbonic acid, diethyl ester (1609-47-8)


Risk and Safety Statements

Signal word  Warning
Hazard statements  H227-H315-H319-H335-H302
Precautionary statements  P301+P312+P330-P210e-P261-P280a-P305+P351+P338-P405-P501a
Hazard Codes  Xn
Risk Statements  22-36/37/38-20/21/22
Safety Statements  26-36-37/39
RIDADR  2810
WGK Germany  3
RTECS  LQ9350000
HazardClass  6.1(b)
PackingGroup  III
HS Code  29209085
Toxicity LD50 orally in Rabbit: 850 mg/kg
NFPA 704
2 1

Diethyl pyrocarbonate price More Price(43)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 8.41751 Diethyl dicarbonate for synthesis 1609-47-8 5ml $31.2 2022-05-15 Buy
Sigma-Aldrich 8.41751 Diethyl dicarbonate for synthesis 1609-47-8 25mL $114 2022-05-15 Buy
Sigma-Aldrich 298711 Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. 1609-47-8 25g $143 2022-05-15 Buy
Sigma-Aldrich 3660-OP OmniPur DEPC - CAS 1609-47-8 - Calbiochem 1609-47-8 25g $239 2022-05-15 Buy
Sigma-Aldrich 298711 Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. 1609-47-8 100g $491 2022-05-15 Buy
Product number Packaging Price Buy
8.41751 5ml $31.2 Buy
8.41751 25mL $114 Buy
298711 25g $143 Buy
3660-OP 25g $239 Buy
298711 100g $491 Buy

Diethyl pyrocarbonate Chemical Properties,Uses,Production

Chemical Properties



Gentle esterifying agent. Preservative for wines, soft drinks, fruit juices.


Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.1


Diethyl pyrocarbonate may be used in the following studies:
  • For the modification of histidyl residues in proteins.
  • As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
  • For the modification of linear and supercoiled plasmid DNAs.
  • As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
  • For cabethoxylation of histidine residues of actin.


ChEBI: The diethyl ester of dicarbonic acid.

General Description

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Safety Profile

Poison by ingestion, inhalation, and intraperitoneal routes. Concentrated DEPC is irritating to eyes, mucous membranes, and sh. When heated to decomposition it emits acrid smoke and fumes. See also ESTERS.

Purification Methods

Dissolve the ester in Et2O, wash it with dilute HCl, H2O, dry over Na2SO4, filter, evaporate and distil the residue first in vacuo then at atmospheric pressure. It is soluble in alcohols, esters, ketones and hydrocarbon solvents. A 50% w/w solution is usually prepared for general use. Treat with great CAUTION as DEP irritates the eyes, mucous membranes and skin. [Boehm & Mehta Chem Ber 71 1797 1938, Thoma & Rinke Justus Liebigs Ann Chem 624 30 1959, Beilstein 3 IV 18.]

Synthesis of Diethyl pyrocarbonate from Ethyl chloroformate

Diethyl pyrocarbonate Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 320)Suppliers
Supplier Tel Email Country ProdList Advantage
Capot Chemical Co.,Ltd.
571-85586718 +8613336195806 China 19994 60
Henan DaKen Chemical CO.,LTD.
+86-371-66670886 China 16666 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693 China 22021 55
21-51619050 China 26815 60
career henan chemical co
+86-0371-86658258 China 29959 58
Hubei Jusheng Technology Co.,Ltd.
86-18871470254 CHINA 28229 58
Hebei Guanlang Biotechnology Co., Ltd.
+86-19930503282 +86-19930503282 China 5953 58
Chongqing Chemdad Co.,Ltd
+86-19923101450 CHINA 4000 58
Hubei xin bonus chemical co. LTD
86-13657291602 CHINA 23035 58
Shandong chuangyingchemical Co., Ltd.
18853181302 CHINA 5917 58

View Lastest Price from Diethyl pyrocarbonate manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2022-02-25 Diethyl pyrocarbonate
US $5.00 / KG/Tin 1KG 99% 10Tons Hebei Senko Biotechnology Co., Ltd.
2021-08-07 Diethyl pyrocarbonate
US $1.00 / KG 1KG 99% 10 mt Hebei Guanlang Biotechnology Co., Ltd.
2021-07-13 Diethyl pyrocarbonate
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
Diethyl dicarbonate for synthesis DEPC, diethypyrocarbonate Diethyl pyrocarbonate Vetec(TM) reagent grade, >=97% diethylesterofpyrocarbonicacid diethylpyrocarbonicacid Diethylpyrokarbonat DKD ethylpyrocarbonate Formic acid, oxydi-, diethyl ester oxydi-formicacidiethylester Piref Pyrocarbonate d'ethyle pyrocarbonated’ethyle Pyrokohlensaeure diaethyl ester DiethylPyrocarbonate(Depc) Oxdiformic acid, diethyl ester Diethyl pyrocarbonate, pure, 97% DIETHYL PYROCARBONATE DIETHYL OXYDIFORMATE ETHOXYFORMIC ACID ANHYDRIDE ETHOXYFORMIC ANHYDRIDE Diethyl Pyrocarbonate (DEPC)(strong product) DIETHYL PYROCARBONATE extrapure DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride DEPC(Diethepyrocarbonate) Diethyl pyrocarbonate ,97% Diethyl pyrocarbonate research grade Diethyl pyrocarbonate,DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride Diethyl pyrocarbonat OXYDIFORMIC ACID DIETHYL ESTER PYROCARBONIC ACID DIETHYL ESTER Baycovin Dicarbonic acid, diethyl ester dicarbonicacid,diethylester dicarbonicaciddiethylester Diethyl ester of pyrocarbonic acid Diethyl pyrocarbonic acid Diethylester kyseliny diuhlicite diethylesterkyselinydiuhlicite Diethyl pyrocarbonate, pure, 97% 25GR Diethyl pyrocarbonate, pure, 97% 5GR Diethyl pyrocarbonate, 97%, pure Pyrocarbonic acid diethyl DEPC (Diethyl pyrocarbonate) Cokecarbonic acidethyl ester two Diethyl pyrocarbonate 99% (NT) Diethyl dicarbonate Diethyl oxydiformate Ethoxyformic acid anhydride Pyrocarbonic acid diethyl ester Diethyl pyrocarbonate, 97%, for bio Dicarbonic acid, 1,3-diethyl ester iethylpyrocarbonate DEP/DEPC Factory supply high quality Diethyl pyrocarbonate CAS 1609-47-8 with good price ethoxycarbonyl ethyl carbonate Diethyl Pyrocarbonate (DEPC) for molecular biology, 99% OmniPur DEPC - CAS 1609-47-8 - Calbiochem Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem Ethyl pyrocarbonate DIETHYL DICARBONATE