Broxuridine | 59-14-3

Broxuridine Properties

Melting point	191-194 °C (dec.)(lit.)
alpha	31 º (c=1, 0.1N NaOH)
Density	1.8573 (rough estimate)
refractive index	22 ° (C=1, H2O)
storage temp.	-20°C
solubility	NH₄OH: 0.1 M at 20 °C, clear, colorless
pka	7.73±0.10(Predicted)
form	Crystals or Crystalline Powder
color	White to yellow
Water Solubility	1-5 g/100 mL at 22 ºC
Merck	14,1452
BRN	30395
Stability	Stable, but may be light sensitive. Incompatible with strong oxidizing agents.
InChIKey	XMJRLEURHMTTRX-UHFFFAOYSA-N
LogP	-0.290
CAS DataBase Reference	59-14-3(CAS DataBase Reference)
NCI Dictionary of Cancer Terms	broxuridine
FDA UNII	G34N38R2N1
NCI Drug Dictionary	bromodeoxyuridine
EPA Substance Registry System	Uridine, 5-bromo-2'-deoxy- (59-14-3)

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS08

Signal word

Danger

Hazard statements

H340-H361fd

Precautionary statements

P201-P202-P280-P308+P313-P405-P501

Hazard Codes

Xn,T

Risk Statements

68-36/37/38-63-46-24-61

Safety Statements

36/37/39-26-53-45-36

WGK Germany

2

RTECS

YU7350000

F

10-23

TSCA

Yes

HS Code

29349990

NFPA 704

	0
1		0

Broxuridine price More Price(74)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	B5002	5-Bromo-2′-deoxyuridine ≥99% (HPLC)	59-14-3	1g	$151	2024-03-01	Buy
Sigma-Aldrich	B5002	5-Bromo-2′-deoxyuridine ≥99% (HPLC)	59-14-3	5g	$598	2024-03-01	Buy
Sigma-Aldrich	203806	5-Bromo-2ʹ-deoxyuridine - CAS 59-14-3 - Calbiochem Thymidineanalog.UsefulforthestudyofDNAsynthesiswhereitisincorporated	59-14-3	1g	$241	2024-03-01	Buy
Sigma-Aldrich	203806	5-Bromo-2ʹ-deoxyuridine - CAS 59-14-3 - Calbiochem Thymidineanalog.UsefulforthestudyofDNAsynthesiswhereitisincorporated	59-14-3	5G	$758	2024-03-01	Buy
Sigma-Aldrich	10280879001	5-Bromo-2′-deoxyuridine	59-14-3	1g	$119	2024-03-01	Buy

Product number	Packaging	Price	Buy
B5002	1g	$151	Buy
B5002	5g	$598	Buy
203806	1g	$241	Buy
203806	5G	$758	Buy
10280879001	1g	$119	Buy

Broxuridine Chemical Properties,Uses,Production

Description

5-Bromo-2'-deoxyuridine is a nitrate reductase inhibitor that prevents the reduction of nitrate to nitrite by inhibiting the enzyme nitrate reductase. 5-Bromo-2'-deoxyuridine is a genotoxic agent that has been shown to cause DNA damage and cell death in vitro. 5-Bromo-2'-deoxyuridine also binds to NMDA receptors and may be useful as a model system for studying neurodegenerative diseases.

Chemical Properties

White crystalline powder

Uses

5-Bromo-2'-deoxyuridine (BrdU) is a thymidine analog used to label DNA. It is incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies to target the BrdU incorporation. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

Uses

A Thymidine analogue used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase

Uses

Research tool for measuring DNA synthesis.

Definition

ChEBI: A pyrimidine 2'-deoxyribonucleoside compound having 5-bromouracil as the nucleobase.

General Description

White crystalline powder.

Air & Water Reactions

Water soluble.

Reactivity Profile

Broxuridine may be heat and light sensitive. .

Health Hazard

ACUTE/CHRONIC HAZARDS: When heated to decomposition Broxuridine emits very toxic fumes of bromide ion and NOx.

Fire Hazard

Flash point data for Broxuridine are not available; however, Broxuridine is probably combustible.

Biological Activity

5-bromodeoxyuridine (5-brdu) is an analog of thy that is incorporated into the dna of cells undergoing the s-phase and can be detected by monoclonal antibodies or polyclona111.

Biochem/physiol Actions

5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation, because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.

Safety Profile

Moderately toxic by subcutaneous, intravenous, intraperitoneal, and possibly other routes. Mildly toxic by ingestion. Experimental teratogenic and reproductive effects. Human mutation data reported. When heated to decomposition it emits very toxic fumes of Brand NOx

in vitro

the effect of 5-brdu on the proliferation was studied in the early phases of dedifferentiation of nicotiana glauca pith explants grown. it was found to be wholly inhibitory only when given during the first 72 h of culture, this inhibition being reversed by simultaneous addition of either deoxyeytidine or thymidine [1].

in vivo

young adult offspring mice were stained for 5-brdu using a monoclonal antibody and the peroxidase-antiperoxidase method. the distribution of immunoreactive nuclei was very similar to the previous [3h] thymidine data. the pattern of labelled nuclei at different embryonic ages followed the spatiotemporal gradients described for cortical and hippocampal neurogenesis. these data indicate that brdu can be used to map neuronal birthdates [2].

storage

-20°C

Purification Methods

Recrystallise the uridine from EtOH or 96% EtOH. It has max 279 nm at pH 7.0, and 279 nm (log 3.95) at pH 1.9. Its RF values are 0.49, 0.46 and 0.53 in n-BuOH/AcOH/H2O (4:1:1), n-BuOH/EtOH/H2O (40:11:19) and i-PrOH-25% aqueous NH3-H2O (7:1:1), respectively. [Nature 209 230 1966, Pryst.s & Sorm Collect Czech Chem Comm 29 2956 1964, Beilstein 24 III/IV 1234.]

References

[1] m. durante, c. geri, v. nuti-ronchi, g. martini, e. guillé, j. grisvard, l. giorgi, r. parenti, m. bulatti. inhibition of nicotiana glauca pich tissue proliferation through incorporation of 5-brdu into sna. cell differentiation, volume 6, issue 1, june 1977, pages 53-63
[2] del rio ja, soriano e. immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. brain res dev brain res. 1989 oct 1; 49 (2): 311-7.

Broxuridine synthesis

Synthesis of Broxuridine from 5-Bromouracil and Thymidine

Broxuridine Preparation Products And Raw materials

Raw materials

Preparation Products

Broxuridine Suppliers

Global( 367)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Wuhu Nuowei chemistry Co., Ltd.	+86-0553-2911116-802 +undefined17756524438	sales1@nuowei-chem.com	China	1642	58
Hebei Mojin Biotechnology Co., Ltd	+8613288715578	sales@hbmojin.com	China	12456	58
Shanghai Daken Advanced Materials Co.,Ltd	+86-371-66670886	info@dakenam.com	China	15928	58
Henan Tianfu Chemical Co.,Ltd.	+86-0371-55170693 +86-19937530512	info@tianfuchem.com	China	21691	55
career henan chemical co	+86-0371-86658258	sales@coreychem.com	China	29914	58
SHANDONG ZHI SHANG CHEMICAL CO.LTD	+86 18953170293	sales@sdzschem.com	China	2931	58
Jinan Carbotang Biotech Co.,Ltd.	+8615866703830	figo.gao@foxmail.com	China	6992	58
Beijing Ribio Biotech Co.,Ltd	010-62664360 +8613328773880	wucy@ribio.com.cn	China	117	58
Yunbio Tech Co.,Ltd.	+86-010-60605551 +86-18046518538	yunbiochem@126.com	China	321	58
Xiamen AmoyChem Co., Ltd	+86-592-6051114 +8618959220845	sales@amoychem.com	China	6387	58

Supplier	Advantage
Wuhu Nuowei chemistry Co., Ltd.	58
Hebei Mojin Biotechnology Co., Ltd	58
Shanghai Daken Advanced Materials Co.,Ltd	58
Henan Tianfu Chemical Co.,Ltd.	55
career henan chemical co	58
SHANDONG ZHI SHANG CHEMICAL CO.LTD	58
Jinan Carbotang Biotech Co.,Ltd.	58
Beijing Ribio Biotech Co.,Ltd	58
Yunbio Tech Co.,Ltd.	58
Xiamen AmoyChem Co., Ltd	58

View Lastest Price from Broxuridine manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2023-10-11	Broxuridine 59-14-3	US $0.00 / KG	1KG	99%	50000KG/month	Hebei Mojin Biotechnology Co., Ltd
2023-09-06	Broxuridine 59-14-3	US $0.00-0.00 / KG	1KG	99%	500000kg	Hebei Guanlang Biotechnology Co., Ltd.
2022-09-30	Broxuridine 59-14-3	US $0.00-0.00 / kg	1kg	98%	1Ton	Henan Aochuang Chemical Co.,Ltd.

Broxuridine
59-14-3
US $0.00 / KG
99%
Hebei Mojin Biotechnology Co., Ltd

Broxuridine
59-14-3
US $0.00-0.00 / KG
99%
Hebei Guanlang Biotechnology Co., Ltd.

Broxuridine
59-14-3
US $0.00-0.00 / kg
98%
Henan Aochuang Chemical Co.,Ltd.

Broxuridine Spectrum

Broxuridine(59-14-3)MS Broxuridine(59-14-3)¹HNMR Broxuridine(59-14-3)¹³CNMR Broxuridine(59-14-3)IR1 Broxuridine(59-14-3)IR2 Broxuridine(59-14-3)Raman

59-14-3(Broxuridine)Related Search:

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Cyanogen bromide 5-Bromouridine 5-Bromo-2'-deoxy-5'-uridylic acid disodium salt 5-Bromouracil Uridine

Uracil Bromine BrdU (Bromodeoxyuridine)

1of4

5-BROMO-1-(2-DEOXY-BETA-D-RIBOFURANOSYL)URACIL 5-BROMO DEOXYURIDINE 5-BROMO-2'-DESOXYURIDINE (+)-5-BROMO-2'-DEOXYURIDINE 5-BROMO-2'-DEOXYURIDINE 5-budr bromouracildeoxyriboside 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil 5-BROMO-2'-DEOXYURIDINE SIGMAULTRA 5-Bromodeoxyuridine,Crystalline,>98% Bromo-2'-deoxyuridine 5-Bromo-2'-deoxy-D-uridine 5-BROMO-2-DEOXYURIDINE,ULTRAPURE 5-BROMO-2''-DEOXYURIDINE(5-BRDU) BROMO-2'-DEOXYURIDINE, 5-(RG) 5-Bromo-2'-deoxyuridine, 99+% BROMODESOXYURIDINE 5-BROMO-2''-DEOXYURIDINE, HPLC PURIFIED, 98% PURE WITH HPLC UV CHROMATOGRAM 5-BROMO-2''-DEOXYURIDINE CRYSTALLINE BROMO-2'-DEOXYURIDINE,5- BRUDR BROXURIDINE BR-DU BRDU LABELING REAGENT CHEMPACIFIC 52436 5-BROMO-2-DEOXYURIDINE extrapure 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR 1-(2-deoxy-β-D-ribofuranosyl)-5-bromouracil 5-bdu 5-bromodesoxyuridine 5-bromouracil-2-deoxyriboside 5-bromouracildeoxyriboside 5''-BROMODEOXYURIDINE (BROXURIDINE) 2'-DEOXY-5-BROMOROURIDINE 2'-DEOXY-5-BROMOURIDINE NSC-3829 radibud 5-Bromo-2’-deoxyridine 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uraci 5-Brdu, 5-Bromo-2'-deoxyuridine (+)-5-BroMo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxyMethyl)oxolan-2-yl]pyriMidine-2,4-dione NSC 38297 5-Br-2'--dU 5-Bromo-1-(2-deoxy-beta-D-ribofuranosyl)uracil 5-BrdU BUdR l 5-Bromouracil deoxyriboside 5-Bromo-2′-deoxyuridine,5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR 5-broMo-1-[(2S,4R,5S)-4-hydroxy-5-(hydroxyMethyl)oxolan-2-yl]-1,2,3,4-tetrahydropyriMidine-2,4-dione 5-BroMo-3-deoxyuridine BrdU [5-Bromo-2'-deoxyuridine] *CAS 59-14-3* 5-BrdU5G Bromodeoxyuridine Cell Proliferation Kit, BioAssay&trade 5-bromo-2-deoxyuridine (BrdU) 5-Bromo-1-(2-deoxy-&beta -D-ribofuranosyl)uracil 5-Bromo-2'-deoxyuridine> Uridine, 5-bromo-2'-deoxy- 5-Bromo-1-(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-ylpyrimidine-2,4-dione Broxuridine USP/EP/BP