Ulex europaeus agglutinin, UEA-I
- CAS No.
- Chemical Name:
- Ulex europaeus agglutinin, UEA-I
- Synonyms
- Ulex europaeus agglutinin, UEA-I;Lectin from Ulex europaeus (gorse, furze)
- CBNumber:
- CB32094561
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Ulex europaeus agglutinin, UEA-I price More Price(1)
Ulex europaeus agglutinin, UEA-I Chemical Properties,Uses,Production
Uses
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Ulex europaeus agglutinin, UEA-I Preparation Products And Raw materials
Raw materials
Preparation Products
Ulex europaeus agglutinin, UEA-I Suppliers
Global( 3)Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Supplier | Advantage |
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Sigma-Aldrich | 80 |
Lectin from Ulex europaeus (gorse, furze)
Ulex europaeus agglutinin, UEA-I