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GLUCOSE-6-PHOSPHATE DEHYDROGENASE

GLUCOSE-6-PHOSPHATE DEHYDROGENASE
GLUCOSE-6-PHOSPHATE DEHYDROGENASE
CAS No.
9001-40-5
Chemical Name:
GLUCOSE-6-PHOSPHATE DEHYDROGENASE
Synonyms
G-6-PD;G-6-P-DH;EC 1.1.1.49;EC: 1.1.1.49;IUB: 1.1.1.49;G-6-PDH TYPE V;ZWISCHENFERMENT;G-6-PDH TYPE IX;G-6-PDH TYPE XV;G-6-pdh control
CBNumber:
CB3211458
Molecular Formula:
n.a.
Formula Weight:
0
MOL File:
Mol file

GLUCOSE-6-PHOSPHATE DEHYDROGENASE Properties

Melting point:
~120℃
storage temp. 
2-8°C
form 
suspension
color 
white
Water Solubility 
Soluble in water and dilute buffer.

SAFETY

Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  26-36-24/25
WGK Germany  3
3-10
TSCA  Yes
HS Code  35079090

GLUCOSE-6-PHOSPHATE DEHYDROGENASE price More Price(43)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 346774 Glucose-6-phosphate Dehydrogenase, 9001-40-5 500u $79 2018-11-20 Buy
Sigma-Aldrich G4134 Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae) 9001-40-5 1ku $493 2018-11-20 Buy
Alfa Aesar J16171 Glucose-6-phosphate dehydrogenase, from Leuconostoc mesenteroides, ammonium sulfate suspension, Affymetrix/USB 9001-40-5 5 kilounit $245 2018-11-15 Buy
Alfa Aesar J16171 Glucose-6-phosphate dehydrogenase, from Leuconostoc mesenteroides, ammonium sulfate suspension, Affymetrix/USB 9001-40-5 1 kilounit $78.5 2018-11-15 Buy
Sigma-Aldrich G7877 Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae) 9001-40-5 5ku $813 2018-11-20 Buy

GLUCOSE-6-PHOSPHATE DEHYDROGENASE Chemical Properties,Uses,Production

Chemical Properties

White to yellow powder

Purification Methods

The enzyme is useful for measuring pyridine nucleotides in enzyme recycling. The enzyme from Baker's yeast has been purified by (NH4)2SO4 fractionation, Me2CO precipitation, a second (NH4)2SO4 fractionation, concentration by DEAE-SF chromatography, a third (NH4)2SO4 fractionation and recrystallisation. Crystallisation is induced by addition of its coenzyme NADP, which in its presence causes rapid separation of crystals at (NH4)2SO4 concentration much below that required to precipitate the amorphous enzyme. To recrystallise, the crystals are dissolved in 0.01M NADP (pH 7.3) with (NH4)2SO4 at 0.55 saturation, and the crystals appear within 10 to 60minutes. After standing for 2-3days (at 4o) the (NH4)2SO4 is increased to 0.60 of saturation, and more than 80% of the activity in the original crystals is recovered in the fresh crystals. [Noltmann et al. J Biol Chem 236 1255 1961]. Large amounts can be obtained from rat livers. The livers are extracted with 0.025M phosphate buffer (pH 7.5) and precipitated with 3M (NH4)2SO4 (70% of activity). The precipitate is dissolved in 3volumes of 0.025M phosphate (pH 7.5), dialysed against this buffer + 0.2mM EDTA at 4o for 5hours, then diluted to 1% protein and the nucleic acids are precipitated by addition of 0.4volumes of 1% protamine sulfate. (NH4)2SO4 is added to a concentration of 2M (pH adjusted to 7.0 with NH3), the precipitate is discarded and the supernatant is adjusted to 2.8M (NH4)2SO4, dialysed, and the protein is adjusted to 1% and treated with Ca3(PO4)2 gel. The gel is added in three steps (1.5mL of 0.4% gel/mL per step), and the gel is removed by centrifugation after each addition. The third gel adsorbed 50% of the activity. The gel is eluted with 0.2M phosphate buffer (pH 7.4, 40mL/g of gel; 60% recovery). The extract is precipitated in 3volumes of (NH4)2SO4 (adjusted to 4M) to give enzyme with an activity of 30Wmoles/mg of protein per hour. [Lowry et al. J Biol Chem 236 2746 1961.] The Km values for the yeast enzyme are 20WM for G-6P and 2WM for NADP (Tris pH 8.0, 10-2 M MgCl2, 38o) [Noltmann & Kuby The Enzymes VII 223 1963].

GLUCOSE-6-PHOSPHATE DEHYDROGENASE Preparation Products And Raw materials

Raw materials

Preparation Products


GLUCOSE-6-PHOSPHATE DEHYDROGENASE Suppliers

Global( 70)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Henan DaKen Chemical CO.,LTD.
+86-371-55531817
info@dakenchem.com CHINA 22049 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com CHINA 20786 55
Shanghai Zheyan Biotech Co., Ltd.
18017610038
zheyansh@163.com CHINA 3624 58
ShangHai YuanYe Biotechnology Co., Ltd. 021-61312847 qq:2797782341
021-55068248 2797782341@qq.com China 7590 60
Shanghai Biological Technology Development Co., Ltd. 400-0909-005 021-65211385-102
021-65211385 lj80414@hotmail.com China 5192 55
J & K SCIENTIFIC LTD. 400-666-7788 +86-10-82848833
+86-10-82849933 jkinfo@jkchemical.com;market6@jkchemical.com China 96815 76
3B Pharmachem (Wuhan) International Co.,Ltd. 86-21-50328103 * 801、802、803、804 Mobile:18930552037
86-21-50328109 3bsc@sina.com China 15926 69
BEST-REAGENT 028-85973071 028-85973075
028-85973075 service@best-reagent.com China 9900 57
Sinopharm Chemical Reagent Co,Ltd. 86-21-63210123
86-21-63290778 86-21-63218885 sj_scrc@sinopharm.com China 9966 79
Dalian Meilun Biotech Co., Ltd. 0411-66771943;0411-66771942
0411-66771945 sales@meilune.com China 4143 58

9001-40-5(GLUCOSE-6-PHOSPHATE DEHYDROGENASE)Related Search:


  • ZWISCHENFERMENT
  • ZWISCHENFERMENT G6P-DH
  • ZWISCHENFERMENT TYPE IX
  • ZWISCHENFERMENT TYPE V
  • ZWISCHENFERMENT TYPE VII
  • ZWISCHENFERMENT TYPE XV
  • ZWISCHENFERMENT TYPE XV-B
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE, LEUCONOSTOC MESENTEROIDES
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE IX
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE V
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE VII
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV-B
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIII
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIV
  • G-6-P-DH
  • G-6-PDH-BIOTIN LABELED
  • G-6-PDH TYPE IX
  • G-6-PDH TYPE V
  • G-6-PDH TYPE VII
  • G-6-PDH TYPE XV
  • G-6-PDH TYPE XV-B
  • IUB: 1.1.1.49
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE X IX
  • G-3-Pdh(Glycerol-3-PhosphateDehydrogenase),Mesenteroides
  • fromyeastsaltfreepowder400unitsmg
  • Glucose-6-phosphate dehydrogenase from yeast ca. 140 U/mg protein
  • D-GLUCOSE-6-PHOSPHATE: NAD(P)+ 1-OXIDOREDUCTASE
  • D-GLUCOSE-6-PHOSPHATE: NADP 1-OXIDOREDUCTASE
  • EC 1.1.1.49
  • EC: 1.1.1.49
  • Dehydrogenase,glucose6-phosphate
  • glucose-6-phosphatedehydrogenasefromyeastsusp~120u/mg
  • G-6-pdh control
  • Glucose-6-phosphate Dehydrogenase from torula yeast
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE FROM &
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TORU&
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE, BAKER 'S YEAST, ~240 U/MG
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE*CONTROL NORMAL
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE BAKE&
  • Glucose-6-phosphate.dehydrogenase from Leuconostoc mesenteroides
  • glucose-6-phosphate dehydrogenase from baker's yeast (s. cerevisiae)
  • Glucose -6-phosphoric acid dehydrogenase
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE extrapure
  • Glucose-6-phosphate Dehydrogenase Biotin Labeled from baker's yeast
  • Glucose-6-phosphate Dehydrogenase from yeast
  • G-6-P-DH, Zwischenferment
  • Glucose-6-phosphate Dehydrogenase-Agarose from baker's yeast (S.cerevisiae)
  • glicose-6-phosphate dehydrogenase, yeast
  • Glucose-6-Phosphate Dehydrogenase,from Yeast, 500U/mg protein
  • Glucose-6-phosphate dehydrogenase [400 units/mg]
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE, TORU- LA YEAST, ~180 U/MG
  • Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae),G-6-P-DH, Zwischenferment
  • Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides,G-6-P-DH
  • Glucose-6-phosphate dehydrogenase [>= 150 units/mg solid]
  • G6P-DH(Glucose-6-Phosphate Dehydrogenase)
  • Glucose-6-Phosphate Dehydrogenase,G6PD
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