Uses
The Cell Proliferation Reagent WST-1 is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.
- Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
- Assessment of growth inhibitory antibodies and physiological mediators
General Description
Cellular proliferation requires the replication of genomic DNA. Thus, monitoring DNA synthesis is an indirect parameter of cell proliferation, as well as being suitable for the study of the regulation of DNA synthesis. [3H]-thymidine has traditionally been used to label the DNA of replicating (cycling) cells. To circumvent the disadvantages associated with the use of the [3H]-thymidine, nonradioactive alternatives based on 5-bromo-2′-deoxyuridine (BrdU) have been developed. The use of chemiluminescence technology provides enhanced sensitivity and a broad measurement range.
