SEPHADEX G-50

SEPHADEX G-50 Chemical Properties,Uses,Production

Description

Sephadex G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.

1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
2. Suitable for purification of DNA from small molecules by gel filtration
3. Small bead size for shorter diffusion distances
4. Smallest bead size in the Sephadex G-50 range

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Uses

The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.

	lower limit	upper limit
dextran gels
Sephadex G-50	1.5	30
Sephadex G-75	3	80
Sephadex G-100	4	150
Sephadex G-150	5	300
Sephadex G-200	5	600
polyacrylamide gels
Bio-Gel P-10	1.5	20
Bio-Gel P-30	2.5	40
Bio-Gel P-60	3	60
Bio-Gel P-100	5	100
Bio-Gel P-150	15	150
BioGel P-200	30	200
Bio-Gel P-300	60	400
dextran-polyacrylamide gels
Sephacryl S-200	5	250
Sephacryl S-300	10	1500
Sephacryl S-400	20	8000
agarose gels
Sepharose 6B	10	4000
Sepharose 4B	60	20,000
Sepharose 2B	70	40,000
Bio-Gel A-0.5	10	500
Bio-Gel A-1.5	10	1500
Bio-Gel A-5	10	5000
Bio-Gel A-15	40	15,000
Bio-Gel A-50	100	50,000

SEPHADEX G-50 Preparation Products And Raw materials

Raw materials

Preparation Products

SEPHADEX G-50 Suppliers

Supplier	Tel	Fax	Email	Country	ProdList	Advantage
Shanghai Zheyan Biotech Co., Ltd.	18017610038		zheyansh@163.com	CHINA	3625	58
future industrial shanghai co., ltd	400-0066-400；021-60496031	021-55660885	sales@jonln.com	China	2042	65
Nanjing Chemlin Chemical Co., Ltd	025-83697070	+86-25-83453306	sales@chemlin.com.cn	China	20023	64
BEST-REAGENT	028-85973071 028-85973075	028-85973075	service@best-reagent.com	China	9968	57
Tianjin heowns Biochemical Technology Co., Ltd.	400 638 7771		sales@heowns.com	China	14543	57
Sinopharm Chemical Reagent Co,Ltd.	86-21-63210123	86-21-63290778 86-21-63218885	sj_scrc@sinopharm.com	China	9975	79
ShangHai LanJi Science and Technology Development Co., Ltd.	021-56373608/66107288/13391393747	021-66107288	liujun@lanji.cn/QQ493513614	China	299	62
Dalian Meilun Biotech Co., Ltd.	0411-66771943；0411-66771942	0411-66771945	sales@meilune.com	China	4377	58
ShangHai YuanYe Biotechnology Co., Ltd.	021-61312847 qq：2797782341	021-55068248	2797782341@qq.com	China	7594	60
Beijing HuaMeiHuLiBiological Chemical	010-56205725;010-86181995	010-65763397	waley188@sohu.com	China	12396	58

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