ChemicalBook >> CAS DataBase List >>Ribonuclease A

Ribonuclease A

CAS No.
9001-99-4
Chemical Name:
Ribonuclease A
Synonyms
RP-1;RNASE;RNAase;S-RNase;Rnase I;RNASE A;abs9330;RNAase A;Amphinase;DNase-free
CBNumber:
CB4131184
Molecular Formula:
NULL
Molecular Weight:
0
MOL File:
Mol file

Ribonuclease A Properties

Melting point 243 °C (dec.)
alpha D25 (per mg N) -0.47° (c = 5)
storage temp. -20°C
solubility Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride
form solid
color white
PH pH : 8.0
Water Solubility Soluble in water.
Sensitive Moisture Sensitive
EWG's Food Scores 1
EPA Substance Registry System Nuclease, ribo- (9001-99-4)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H319
Precautionary statements  P305+P351+P338
Safety Statements  22-24/25
WGK Germany  3
RTECS  RF0760000
10-21
TSCA  Yes
HS Code  35079090

Ribonuclease A price More Price(65)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich RNASEA-RO RNase A from bovine pancreas 9001-99-4 25 mg $55.4 2022-05-15 Buy
Sigma-Aldrich RNASEA-RO RNase A from bovine pancreas 9001-99-4 100 mg $118 2022-05-15 Buy
Sigma-Aldrich R7884 Ribonuclease B from bovine pancreas BioReagent,≥50?Kunitzunits/mgprotein,≥80%(SDS-PAGE) 9001-99-4 500 μg $46.4 2022-05-15 Buy
Sigma-Aldrich R1153 RNase B Glycoprotein Standard from bovine pancreas Proteomics Grade 9001-99-4 500 μg $310 2022-05-15 Buy
Sigma-Aldrich 55674-M RNaseA, Bovine Pancreas 9001-99-4 55674-50KU $335 2022-05-15 Buy
Product number Packaging Price Buy
RNASEA-RO 25 mg $55.4 Buy
RNASEA-RO 100 mg $118 Buy
R7884 500 μg $46.4 Buy
R1153 500 μg $310 Buy
55674-M 55674-50KU $335 Buy

Ribonuclease A Chemical Properties,Uses,Production

Chemical Properties

Lyophilized

Uses

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.

Uses

Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas.

Uses

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.

General Description

Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Purification Methods

Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins.

Ribonuclease A Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 267)Suppliers
Supplier Tel Email Country ProdList Advantage
Henan DaKen Chemical CO.,LTD.
+86-371-66670886 info@dakenchem.com China 16666 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693 info@tianfuchem.com China 22021 55
career henan chemical co
+86-0371-86658258 sales@coreychem.com China 29959 58
GIHI CHEMICALS CO.,LIMITED
08657186217390 sales@gihichemicals.com CHINA 310 58
Hubei xin bonus chemical co. LTD
86-13657291602 linda@hubeijusheng.com CHINA 23035 58
Chongqing Chemdad Co., Ltd
+8613650506873 sales@chemdad.com CHINA 37442 58
CONIER CHEM AND PHARMA LIMITED
+8618523575427 sales@conier.com China 47501 58
Zhuozhou Wenxi import and Export Co., Ltd
+8613111626072 zzwenxi@126.com CHINA 13187 58
Creative Enzymes
631-6197922 info@creative-enzymes.com United States 8748 58
Hubei Ipure Biology Co., Ltd
+8618062405514 ada@ipurechemical.com China 10352 58

View Lastest Price from Ribonuclease A manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2021-09-29 Ribonuclease A
9001-99-4
US $1.00 / g 1g ≥60u/mg 10kg/month WUHAN FORTUNA CHEMICAL CO., LTD
2021-07-13 Ribonuclease A
9001-99-4
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
2021-07-10 Ribonuclease A
9001-99-4
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
  • Ribonuclease A
    9001-99-4
  • US $1.00 / g
  • ≥60u/mg
  • WUHAN FORTUNA CHEMICAL CO., LTD
  • Ribonuclease A
    9001-99-4
  • US $15.00-10.00 / KG
  • 99%+ HPLC
  • Zhuozhou Wenxi import and Export Co., Ltd
  • Ribonuclease A
    9001-99-4
  • US $15.00-10.00 / KG
  • 99%+ HPLC
  • Zhuozhou Wenxi import and Export Co., Ltd
Ribonuclease A froM bovine pancreas Min. 70 Kunitz units/Mg lyophil. Ribonuclease A froM bovine pancreas Min. 80 Kunitz units/Mg lyophil. Ribonuclease A froM bovine pancreas(Purified) Ribonuclease A from bovine pancreas Vetec(TM) reagent grade, 80% bonuclease A O-PHENYLENEDIAMINE REAGENT GRADE RIBONUCLEASE A FROM BOVINE PANCREAS, POW DER, ~80 U/MG RIBONUCLEASE A SOLUTION MOLECULAR*BIOLOG Y REAGENT RNASE A, BOVINE PANCREAS RibonucleaseDExBovinePancreas RibonucleaseExBovinePancreas RibonucleaseAExBovinePancreas DNase-free Ribonuclease A from bovine pancreas ca. 70 Kunitz units/mg Ribonuclease A from bovine pancreas ca. 90 Kunitz units/mg ribonuclease s from bovine pancreas rnase b glycoprotein standard from bovine pancreas RNASEA,LYOPHILIZEDPOWDER,HIGHPURITY RP-1 Amphinase S-RNase RNase A (from bovine pancreas) RIBONUCLEASE extrapure RibonucleaseA, with scrambled disulfide bonds from bovine pancreas Ribonuclease A-agarose Ribonuclease I, Ribonucleate 3μ-pyrimidinooligonucleotidohydrolase Rnase I ribornuclease RNAase RIBONUCLEASE A FROM BOVINEPANCREAS CA.90 KUNITZ-U/MG LYOPHIL. RIBONUCLEASE A BOVINE PANCR.CA.70 KUNITZ-U/MG LYOPHIL. RNase B Glycoprotein Standard from bovine pancreas,RNase B, Ribonuclease B from bovine pancreas Ribonuclease A from bovine pancreas,Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase TBS BUFFER 20X LIQUID ULTRA PURE GRADE RIBONUCLEASE A FROM BOVINE PANCREAS, POW DER, ~100 U/MG RIBONUCLEASE B PROTEOMICS GRADE RIBONUCLEASE A TYPE X-A: SOLUTION FROM*B OVINE PANCR RIBONUCLEASE A FROM BOVINE PANCREAS, IN 0.2 M SO-PHOSPHATE* RIBONUCLEASE A FROM BOVINE PANCREAS, >70 U/MG* RIBONUCLEASE A FROM BOVINE PANCREAS, TYPE 1-A Ribonuclease A from bovine pancreas, lyophil. ribonuclease A solution molecular*biology ribonuclease A type I-A from*bovine pancreas ribonuclease A type I-as from*bovine pancreas ribonuclease A type ii-A from*bovine pancreas ribonuclease A type iii-A from*bovine pancreas ribonuclease A type X-A: solution from*bovine pan ribonuclease A type xii-A from bovine*pancreas Ribonuclease A, Protease-Free ribonuclease from aspergillus clavatus ribonuclease S grade xii-S Nuclease, ribo- RIBONUCLEASE A, CHROMATOGRAPHICALLY*PURI FIED, MOLEC RIBONUCLEASE A FROM BOVINE PANCREAS 4XCR SALTFREE ~70 U/MG RIBONUCLEASE A FROM BOVINE PANCREAS, ~90 U/MG Ribonuclease B from bovine pancreas,RNase B Ribonuclease from Bovine Pancreas Ribonuclease A from bovine pancreas min. 80 Kunitz units/mg