T7 RNA POLYMERASE
- CAS No.
- Chemical Name:
- T7 RNA POLYMERASE
- Synonyms
- T7 RNA;abs60153;T7 POLYMERASE;T7 RNA POLYMERASE
- CBNumber:
- CB4167044
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MFCD00166013
- MOL File:
- Mol file
T7 RNA POLYMERASE price More Price(5)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich | RPOLT7-RO | T7 RNA Polymerase from Escherichia coli BL 21/pAR 1219 | 1000units | $170 | 2024-03-01 | Buy | |
Sigma-Aldrich | RPOLT7-RO | T7 RNA Polymerase from Escherichia coli BL 21/pAR 1219 | 5000units | $482 | 2024-03-01 | Buy | |
American Custom Chemicals Corporation | ENZ0000893 | T7 RNA POLYMERASE 95.00% | 5MG | $495.08 | 2021-12-16 | Buy | |
Biorbyt Ltd | orb533412 | T7 RNA Polymerase | 20000units | $404.6 | 2021-12-16 | Buy | |
Biorbyt Ltd | orb533412 | T7 RNA Polymerase | 4000units | $125.8 | 2021-12-16 | Buy |
T7 RNA POLYMERASE Chemical Properties,Uses,Production
characteristic
T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis. T7 RNA Polymerase is provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM spermidine, 50mM NaCl.
Description
T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology.
Uses
- T7 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T7 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including: RNA or DNA blotting techniques
- In situ hybridization
- RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
- Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
- Microarray target synthesis
Application
Radiolabeled RNA probe preparation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
mRNA, sgRNA synthesis
General Description
T7 RNA polymerase is commonly used to transcribe DNA which has been cloned into vectors which have two phage promoters in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with different polymerases. Homogeneously labeled single-stranded RNA can be generated with this system. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S]-labeled nucleotides.
Synthesis of hybridization probes: T7 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels:Transcripts can be nonradioactively labeled with biotin-16-UTP or DIG-11-UTP. They may also be radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides.
T7 RNA POLYMERASE Preparation Products And Raw materials
Raw materials
Preparation Products
T7 RNA POLYMERASE Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Hzymes Biotechnology Co. LTD | +86-02160879177 +86-13849862354 | zhongtingting@hzymes.com | China | 116 | 58 |
Yinjia (Shanghai) Bio-pharmaceutical Technology Co. , Ltd. | 021-52992719 13512169707 | product@yinjibio.com | China | 29 | 58 |
Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Shanghai Kanglang Biotechnology Co., Ltd. | 021-61998208 18217752821 | sales@klbio.cn | China | 9617 | 58 |
Absin Bioscience Inc. | 021-38015121 15000105423 | chenjw@absin.cn | China | 24734 | 58 |
Wuhan Chemstan Biotechnology Co., Ltd. | 15926423062 | 422450190@qq.com | China | 10132 | 58 |
Shanghai Universal Biotech Co.,Ltd | 18768175414 | gaojun@univ-bio.com | China | 24998 | 58 |
Jiangsu Synthgene Biotechnology Co.,Ltd. | 18136497538 | znf@syngenebio.com | China | 901 | 58 |
Wuhan Xinzhecheng Technology Co., Ltd | 17720588118 | 2442693357@qq.com | China | 144 | 58 |
Shanghai Chemilab Biotechnology CO.,Ltd | 18116465878 | kmlb@kaimilaibo.com | China | 2401 | 58 |
View Lastest Price from T7 RNA POLYMERASE manufacturers
Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
---|---|---|---|---|---|---|---|---|
2022-06-15 | T7 RNA polymerase | US $0.00-0.00 / ku | 5ku | ≥95% | 5000ku | Hzymes Biotechnology Co. LTD |
- T7 RNA polymerase
- US $0.00-0.00 / ku
- ≥95%
- Hzymes Biotechnology Co. LTD
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