Uses
DIG Luminescent Detection Kit has been used for highly sensitive and specific detection of DIG-labeled nucleic acids in:
- critical commercial assays
- Southern hybridization
- northern analysis
- plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.
General Description
DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. The nonradioactive DIG system uses digoxigenin, a steroid hapten, coupled to deoxyuridine triphosphate (dUTP), UTP or didUTP to label DNA, RNA or oligonucleotides for hybridization and subsequent luminescent detection.
This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
