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Phenazine methosulfate

CAS No.
299-11-6
Chemical Name:
Phenazine methosulfate
Synonyms
PMS;Phenazine methosuL;5-Methylphenazinium;Phenazine methosulfa;PHENAZINE METHOSULFATE;Phenazine Methoshlphate;PHENAZINE METHOSULPHATE;PHENAZINE METHOLSULPHATE;PHENAZINE METHYL SULFATE;PhenazineMethylSulfate>
CBNumber:
CB4315528
Molecular Formula:
C14H14N2O4S
Molecular Weight:
306.34
MDL Number:
MFCD00011923
MOL File:
299-11-6.mol
MSDS File:
SDS
Last updated:2024-03-27 13:09:27

Phenazine methosulfate Properties

Melting point 158-160 °C (dec.)(lit.)
Density 1.3395 (rough estimate)
refractive index 1.6930 (estimate)
storage temp. -20°C
solubility H2O: 0.2 g/mL at 20 °C, clear, deep orange
form Crystalline Powder
color Dark yellow to brown or khaki
Water Solubility soluble in water (approximately 200 mg/ml).
Sensitive Light Sensitive
Merck 14,6109
BRN 3898869
Stability Stable. Incompatible with strong oxidizing agents.
CAS DataBase Reference 299-11-6(CAS DataBase Reference)
FDA UNII J3GYQ3401Y
EPA Substance Registry System Phenazinium, 5-methyl-, methyl sulfate (299-11-6)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P261-P280a-P304+P340-P305+P351+P338-P405-P501a
Hazard Codes  Xi,Xn,T
Risk Statements  36/37/38-68-46
Safety Statements  26-36/37/39-53-45
RIDADR  2811
WGK Germany  3
RTECS  SG1645000
8
TSCA  Yes
HS Code  29339900
NFPA 704
1
2 0

Phenazine methosulfate price More Price(26)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich P9625 Phenazine methosulfate ≥90% (UV) 299-11-6 1g $59.6 2024-03-01 Buy
Sigma-Aldrich P9625 Phenazine methosulfate ≥90% (UV) 299-11-6 5g $240 2024-03-01 Buy
TCI Chemical P0083 Phenazine Methyl Sulfate >98.0%(HPLC)(N) 299-11-6 1g $32 2024-03-01 Buy
TCI Chemical P0083 Phenazine Methyl Sulfate >98.0%(HPLC)(N) 299-11-6 5g $91 2024-03-01 Buy
Alfa Aesar H56718 Phenazine methosulfate, 98+% 299-11-6 5g $133 2024-03-01 Buy
Product number Packaging Price Buy
P9625 1g $59.6 Buy
P9625 5g $240 Buy
P0083 1g $32 Buy
P0083 5g $91 Buy
H56718 5g $133 Buy

Phenazine methosulfate Chemical Properties,Uses,Production

Description

Phenazine is a free radical generator. It has been used as an electron transfer reactant in cell viability assays. Phenazine (10 μM) induces ssDNA break formation in the presence of the reducing agent NADPH in a cell-free plasmid cleavage assay when used at a concentration of 10 μM. It induces oxidative DNA damage in an alkaline comet assay and apoptosis in A375 melanoma cells when used at a concentration of 10 μM. Phenazine (20 nM) oxidizes cysteine-containing proteins in HepG2 cells.

Chemical Properties

orange to light brown crystals or powder

Uses

Phenazine Methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidized by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported.

Definition

ChEBI: 5-methylphenazinium methyl sulfate is an azaheterocycle sulfate salt and a member of phenazines. It is used as an electron carrier in place of the flavine enzyme of Warburg in the hexosemonophosphate system and also in the preparation of succinic dehydrogenase. Green et al., J. Biol. Chem. 217, 551 (1955).

Application

Phenazine methosulfate is a compound used to determine nitric oxide reductase activity. Phenazine methosulfate has been used:
as a component of succinate dehydrogenase enzyme substrate solution.
to induce superoxide radical generation in red blood cell suspensions and to study its influence on RBC deformability.
as a component of complex II histochemistry media.
Used in the discovery of potent bromophenazine antibacterial agents against Staphylococcus.

General Description

Phenazines are heterocyclic compounds produced as secondary metabolites by bacteria.

Biochem/physiol Actions

Phenazine methosulfate (PMS) acts as a good electron acceptor. PMS is reduced non-enzymatically by (nicotinamide adenine dinucleotide) NADH and (nicotinamide adenine dinucleotide phosphate) NADPH.

Enzyme inhibitor

This redox-active reagent (FW = 306.34 g/mol; CAS 299-11-6; Eo’ = +0.080 V, pH = 7 and T = 30°C), also known as N-methylphenazoniummethosulfat, is frequently used as an artificial electron acceptor and carrier in studies of redox reactions. The reduced semiquinone, which may be prepared nonenzymatically from NADH or NADPH, is a colorless product (occasionally, a green color is reported) and can be used as an electron donor. This reduced compound is rapidly oxidized by dioxygen and will reduce cytochrome c, indophenol dyes, and many other electron acceptors. It is often used with ascorbic acid to determine nitric oxide reductase activity. Action as a Redox Substrate: Phenazine methosulfate (MTT) is a synthetic electron acceptor substtrate for many enzymes (e.g., succinate dehydrogenase, holine dehydrogenase, glycolate dehydrogenase, polyvinylalcohol dehydrogenase, (R)-pantolactone dehydrogenase, formate dihydrogenase, isoquinoline 1-oxidoreductase, quinaldate 4-oxidoreductase, aralkylamine dehydrogenase, glycine dehydrogenase (cyanide-forming), trimethylamine dehydrogenase, cytokinin dehydrogenase, and 4-cresol dehydrogenase (hydroxylating). When used in enzyme assays, MTT is converted to formazin, an intensely purple-colored product. To achieve high-sensitivity and a linear dependence, one must use a solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) to disperse/dissolve the otherwise insoluble formazan to obtain colored suspension/solution. The absorbance of this solution may then be quantified by measuring the wavelength between 500 and 600 nm in a spectrophotometer. The degree of light absorption depends on the solvent. Cell Viability Asaays: MTT has been widely employed in colorimetric assays for assessing the viability of cells. When tested under defined conditions, NAD(P)H-dependent cellular oxidoreductase enzymes catalyze the conversion of MTT to formazan, the intensity of which indicates cell viability. Other related tetrazolium dyes (including XTT, MTS and the WSTs) are used in conjunction with the intermediate electron acceptor, 1- methoxy phenazine methosulfate (PMS). Target(s): ferredoxin:NADPcyclase; photophosphorylation; progesterone monooxygenase; protein-Np -phosphohistidine:sugar phosphotransferase; stearoyl-CoA 9- desaturase; steroid 9a-monooxygenase; steroid 11bmonooxygenase; and testosterone 5a-reductase.

Purification Methods

It forms yellow-brown prisms from EtOH (charcoal), or EtOH/Et2O. Its solubility in H2O at 20o is 10%. In the presence of aqueous KI it forms a semiquinone which crystallises as blue leaflets from EtOH. [Wieland & Roseen Chem Ber 48 1117 1913, Voriskova Collect Czech Chem Commun 12 607 1947, Bülow Chem Ber 57 1431 1924, Campbell et al. J Chem Soc 404 1938, Morley J Chem Soc 4008 1952, Beilstein 23 I 59, 23 II 234, 23 III/IV 1658, 23/8 V 395.]

120-80-9
299-11-6
Synthesis of Phenazine methosulfate from Catechol
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View Lastest Price from Phenazine methosulfate manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Phenazine methylsulfate pictures 2024-03-27 Phenazine methylsulfate
299-11-6
US $50.00 / kg 100kg 99% 1000kg Shaanxi Franta Biotechnology Co., Ltd
5-Methylphenazinium methosulfate pictures 2023-11-09 5-Methylphenazinium methosulfate
299-11-6
US $800.00 / kg 1kg 99% 10tons qingdao future trading Co., Ltd
Phenazine methylsulfate pictures 2023-10-20 Phenazine methylsulfate
299-11-6
US $90.00-70.00 / kg 1kg 99% 10000/month Anhui Zhongda Biotechnology Co., Ltd
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