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CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY

CAS No.
108750-13-6
Chemical Name:
CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY
Synonyms
Calcein Sodium Salt, ca 2Na;Calcein sodium salt, ca 2-3 Na;Calcein Disodium Salt, For complexometry;CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY;Sodium 2',7'-bis((bis(carboxymethyl)amino)methyl)-3'-hydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6'-olate;Bis[N,N-di(carboxymethyl)aminomethyl]fluorescein disodium salt, Fluorescein-bismethyliminodiacetic acid disodium salt
CBNumber:
CB4972574
Molecular Formula:
C30H27N2NaO13
Molecular Weight:
646.54
MDL Number:
MFCD00005049
MOL File:
108750-13-6.mol
MSDS File:
SDS
Last updated:2023-04-23 13:52:06

CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY Properties

color Dark Red
Water Solubility Soluble in water.

SAFETY

Risk and Safety Statements

NFPA 704
1
0 0

CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
TRC C125403 CalceinSodiumSalt 108750-13-6 1g $125 2021-12-16 Buy
American Custom Chemicals Corporation FRL0000068 CALCEIN SODIUM SALT 95.00% 108750-13-6 5G $963.05 2021-12-16 Buy
Product number Packaging Price Buy
C125403 1g $125 Buy
FRL0000068 5G $963.05 Buy

CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY Chemical Properties,Uses,Production

Uses

Calcein Disodium Salt can be used in therapeutic use and biological study of induction of multixenobiotic defense mechanisms through ABC transporters and stress protein hsp70 in resistant Daphnia magna clones as general cellular response to stress.

Purification Methods

Dissolve it in distilled H2O and acidify with dilute HCl to pH 3.5. Filter off the solid acid and wash it well with H2O. Redissolve ca 10g in 300mL H2O containing 12g of NaOAc. Precipitate it again by adding HCl, filtering and washing with H2O. Add the solid to 200mL of EtOH stir for 1hour and filter. Repeat the EtOH wash and dry the bright yellow solid in a vacuum. This acid decomposes on heating at ca 180o. See below for the preparation of the Na salt. [Diehl & Ellingboe Anal Chem 28 882 1956]. Altenatively dissolve it in H2O and acidify with 3N HCl to pH 3.5. Collect the solid and wash it with H2O. The air-dried precipitate is extracted with 70% aqueous EtOH, filtered hot and cooled slowly. Fine yellow needles of the acid crystallise out; they are filtered off and dissolved in the minimum quantity of 0.01N NaOH and re-precipitated by adding N HCl to pH 3.5. It is then recrystallised from 70% aqueous EtOH (3x). The final product (acid) is dried at 80o in a vacuum for 24hours, m >300o(dec). It contains one molecule of water per molecule of acid (C30H36N4O13.H2O). The product is pure as revealed by electrophoresis at pH 5.6 and 8.6, and by TLC in i-BuOH/i-PrOH/AcOH/H2O (60:60:5:5 by volume) or i-PrOH or pH 8.0 borate buffer. [Wallach et al. Anal Chem 31 456 1959.] The Na salt is prepared by dissolving the pure acid in H2O containing 2 mols of NaOH per mol of acid reagent and lyophilising. It complexes with Ca and Mg ions. [Beilstein 19 III/IV 4338.]

CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY pictures 2019-07-06 CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY
108750-13-6
US $1.00 / KG 1G 99% 100KG Career Henan Chemical Co
CALCEIN DISODIUM SALT, INDICATOR FOR COM PLEXOMETRY Calcein sodium salt, ca 2-3 Na Bis[N,N-di(carboxymethyl)aminomethyl]fluorescein disodium salt, Fluorescein-bismethyliminodiacetic acid disodium salt Calcein Disodium Salt, For complexometry Calcein Sodium Salt, ca 2Na Sodium 2',7'-bis((bis(carboxymethyl)amino)methyl)-3'-hydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6'-olate 108750-13-6 C30H24N2Na2O13