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BCECF-AM

BCECF-AM structure

CAS No.: 117464-70-7

Chemical Name:: BCECF-AM

Synonyms: BCECF-AM;BCECF--1M;BCECF--1M 1MG;Bcecf-AmSolution;bcecf-acetoxymethyl;BCECF-Acetoxymethyl ester;BCECF/AM - CAS 117464-70-7 - Calbiochem;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN;2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei

CBNumber:: CB5111514

Molecular Formula:: C42H40O21

Molecular Weight:: 880.75

MDL Number:: MFCD00036969

MOL File:: 117464-70-7.mol

Last updated:2023-05-04 15:15:46

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BCECF-AM Properties

Boiling point	692.41°C (rough estimate)
Density	1.2837 (rough estimate)
refractive index	1.6500 (estimate)
Flash point	85 °C
storage temp.	-20°C
form	White to off-white solid
CAS DataBase Reference	117464-70-7(CAS DataBase Reference)

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H319-H315-H335
Precautionary statements	P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	22-26-36
WGK Germany	3

BCECF-AM price More Price(14)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	216254	BCECF/AM	117464-70-7	1mg	$193	2024-03-01	Buy
Cayman Chemical	15922	BCECF-Acetoxymethyl ester ≥95%	117464-70-7	1mg	$117	2024-03-01	Buy
Cayman Chemical	15922	BCECF-Acetoxymethyl ester ≥95%	117464-70-7	5mg	$521	2024-03-01	Buy
Cayman Chemical	15922	BCECF-Acetoxymethyl ester ≥95%	117464-70-7	25mg	$1540	2021-12-16	Buy
TRC	B119183	BCECF/AM	117464-70-7	1mg	$220	2021-12-16	Buy

Product number	Packaging	Price	Buy
216254	1mg	$193	Buy
15922	1mg	$117	Buy
15922	5mg	$521	Buy
15922	25mg	$1540	Buy
B119183	1mg	$220	Buy

BCECF-AM Chemical Properties,Uses,Production

Description

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.

Chemical Properties

pale yellow to yellow powder

Uses

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

General Description

Material is an isomeric mixture of lactones, acetates, and AM esters

Biological Activity

Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.

BCECF-AM Preparation Products And Raw materials

Raw materials

Preparation Products

BCECF-AM Suppliers

Global( 122)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	49390	58
TargetMol Chemicals Inc.	+1-781-999-5354 +1-00000000000	marketing@targetmol.com	United States	19892	58
Alfa Chemistry	+1-5166625404	Info@alfa-chemistry.com	United States	21317	58
LEAPCHEM CO., LTD.	+86-852-30606658	market18@leapchem.com	China	43348	58
Aladdin Scientific	+1-833-552-7181	sales@aladdinsci.com	United States	57511	58
Aladdin Scientific	+1-833-552-7181	sales@aladdinsci.com	United States	52927	58
Mainchem Co., Ltd.	+86-0592-6210733	sale@mainchem.com	China	32360	55
3B Pharmachem (Wuhan) International Co.,Ltd.	821-50328103-801 18930552037	3bsc@sina.com	China	15848	69
BeiJing Hwrk Chemicals Limted	0757-86329057 18934348241	sales4.gd@hwrkchemical.com	China	14067	55
Beijing Ouhe Technology Co., Ltd	010-82967028 13552068683	2355560935@qq.com	China	12426	60

Supplier	Advantage
CONIER CHEM AND PHARMA LIMITED	58
TargetMol Chemicals Inc.	58
Alfa Chemistry	58
LEAPCHEM CO., LTD.	58
Aladdin Scientific	58
Aladdin Scientific	58
Mainchem Co., Ltd.	55
3B Pharmachem (Wuhan) International Co.,Ltd.	69
BeiJing Hwrk Chemicals Limted	55
Beijing Ouhe Technology Co., Ltd	60

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2',7'-BIS-(2-CARBOXYETHYL)-5-(AND-6)-CARBOXYFLUORESCEIN, ACETOXYMETHYL ESTER 2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN 2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER 2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER 2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO Bcecf-AmSolution 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester mixed isomers bcecf-acetoxymethyl 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein AM ester BCECF-AM solution, Spiro(isobenzofuran-1(3H),9μ-(9H)xanthene)-2μ,7μ-dipropanoic acid 2',7'-Bis(2-acetoxymethoxycarbonylethyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid acetoxymethyl ester 2,7-Bis[2-(acetoxymethoxycarbonyl)ethyl]-3,6-dihydroxy-3'-oxospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-6'-carboxylic acid acetoxymethyl ester 2',7'-Biscarboxyethyl-6-carboxyfluorescein acetoxymethyl ester BCECF-AM 3',6'-bis(Acetyloxy)-5(or6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid2',7'-bis[(acetyloxy)methyl]ester BCECF-Acetoxymethyl ester BCECF--1M BCECF--1M 1MG SPIRO(ISOBENZOFURAN-1(3H),9'-(9H)XANTHENE)-2',7'-DIPROPANOIC ACID 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxyMet BCECF/AM - CAS 117464-70-7 - Calbiochem 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethy 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester 117464-70-7 C42H40O21 C39H36O19 Ion Channels Monovalent Ion Channels Voltage-gated Ion Channels Cell Signaling and Neuroscience BioChemical Cell Biology Ion Probes