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Biocytin

CAS No.
576-19-2
Chemical Name:
Biocytin
Synonyms
BCT;BIOCYTIN;L-BIOCYTIN;d-Biocytin;NεH-LYS(BIOT)-OH;H-Lys(biotinyl);H-LYS(BIOTINYL)-OH;BIOCYTIN FREE BASE;Biocytin (&epsilon
CBNumber:
CB5184561
Molecular Formula:
C16H28N4O4S
Formula Weight:
372.48
MOL File:
576-19-2.mol
MSDS File:
SDS

Biocytin Properties

Melting point ~245 °C (dec.) (lit.)
alpha D25 +53° (c = 1.05 in 0.1N NaOH)
Boiling point 748.0±60.0 °C(Predicted)
Density 1.234
storage temp. -20°C
pka 2.53±0.24(Predicted)
optical activity [α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)
Merck 13,1227
BRN 97197
InChIKey BAQMYDQNMFBZNA-MNXVOIDGSA-N
FDA UNII G6D6147J22

SAFETY

Risk and Safety Statements

WGK Germany  3
HS Code  29362900

Biocytin price More Price(33)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich B4261 Biocytin ≥98% (TLC) 576-19-2 5mg $50.5 2022-05-15 Buy
Sigma-Aldrich B4261 Biocytin ≥98% (TLC) 576-19-2 25mg $92.6 2022-05-15 Buy
Cayman Chemical 16751 Biocytin ≥98% 576-19-2 10mg $32 2022-04-27 Buy
Cayman Chemical 16751 Biocytin ≥98% 576-19-2 25mg $63 2022-04-27 Buy
Sigma-Aldrich B4261 Biocytin ≥98% (TLC) 576-19-2 50mg $137 2022-05-15 Buy
Product number Packaging Price Buy
B4261 5mg $50.5 Buy
B4261 25mg $92.6 Buy
16751 10mg $32 Buy
16751 25mg $63 Buy
B4261 50mg $137 Buy

Biocytin Chemical Properties,Uses,Production

Description

Biocytin is a conjugate of biotin and lysine known formally as ?-N-(d-biotinyl)-L-lysine. The addition of lysine to biotin increases the chain length extending from biotin, allowing the synthesis of medium-chain reagents. It also provides terminal carboxyl and amino groups for derivatization or conjugation to proteins and other molecules. Covalent linking reactions typically involve carbodiimide or NHS-ester crosslinking chemistries. Biocytin is also used as an anterograde, retrograde, or intracellular neuroanatomical tracer that is fixable with aldehyde-based fixatives. For this and other applications, biocytin is detected with avidin or streptavidin probes.

Chemical Properties

White Solid

Uses

Useful for the synthesis of Biocytin containing peptides to study the mechanism of reactions catalyzed by biotin - containing enzymes

Uses

Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.

Uses

Biocytin can be used as a versatile fluorescent marker for neuroanatomical investigations and for a biotinidase assay.

Definition

ChEBI: A monocarboxylic acid amide that results from the formal condensation of the carboxylic acid group of biotin with the N6-amino group of L-lysine.

Biological Activity

Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Biochem/physiol Actions

Biocytin is an adduct of lysine and biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme.

Purification Methods

Recrystallise biocytin rapidly from dilute MeOH or Me2CO. It can also be recrystallised from H2O by slow evaporation or by dissolving in the minimum volume of H2O and adding Me2CO until solid separates. It is freely soluble in H2O and AcOH but insoluble in Me2CO. [Wolf et al. J Am Chem Soc 74 2002 1952, 72 1048 1950.] It has been purified by chromatography on superfiltrol-Celite, Al2O3 and by countercurrent distribution and then recrystallised [IR: Peck et al. J Am Chem Soc 74 1991 1952]. The hydrochloride recrystallises from aqueous Me2CO/HCl and has m 227o (dec). [Beilstein 27 III/IV 7984.]

Biocytin Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 136)Suppliers
Supplier Tel Email Country ProdList Advantage
ATK CHEMICAL COMPANY LIMITED
21-51619050 ivan@atkchemical.com China 26810 60
GenicBio Limited
+86-21-37621270 service@genicbio.com CHINA 991 58
Hubei xin bonus chemical co. LTD
86-13657291602 linda@hubeijusheng.com CHINA 23035 58
HENAN BON INDUSTRIAL CO.,LTD
0371-55170695 info@hnbon.com CHINA 26742 58
CONIER CHEM AND PHARMA LIMITED
+8618523575427 sales@conier.com China 47500 58
career henan chemical co
+86-0371-86658258 factory@coreychem.com China 29862 58
Guangzhou Yuheng Pharmaceutical Technology Co., Ltd
+8613580539051 joe@yuhengpharm.com CHINA 21151 58
Career Henan Chemica Co
+86-0371-86658258 laboratory@coreychem.com China 30290 58
GL Biochem (Shanghai) Ltd.
+86-021-61263370 +86-18901917034 xingli@glbiochem.com CHINA 3905 58
GL Biochem(Binhai) Ltd.
021-61263370 +8615301650015 wuqiuyan@glschina.com CHINA 2976 58

View Lastest Price from Biocytin manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2022-02-19 H-Lys(biotinyl)-OH
576-19-2
US $0.00 / KG 1KG 98.9% 100 tons Honest Joy Holdings Limited
2020-01-09 Biocytin
576-19-2
US $1.00 / g 1g ≥98% g/kg/Ton Career Henan Chemical Co
  • Biocytin
    576-19-2
  • US $1.00 / g
  • ≥98%
  • Career Henan Chemical Co
NE-BIOTYNYL-L-LYSINE N-EPSILON-BIOTIN-L-LYSINE N-EPSILON-(+)-BIOTINYL-L-LYSINE N-EPSILON-BIOTINYL-L-LYSINE N-EPSILON-D-BIOTINYL-L-LYSINE LYSINE(BIOTINYL)-OH L-BIOCYTIN EPSILON-BIOTINOYL-L-LYSINE H-LYS(BIOTINYL)-OH H-LYS(BIOT)-OH BIOCYTIN BCT BIOCYTIN FREE BASE (2S)-2-amino-6-[5-[(1S,2S,5R)-7-oxo-3-thia-6,8-diazabicyclo[3.3.0]oct-2-yl]pentanoylamino]hexanoic acid N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine (S)-2-aMino-6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]iMidazol-4-yl)pentanaMido)hexanoic acid Biocytin (ε-Biotinoyl-L-lysine) Bct, Nε-(+)-Biotinyl-L-lysine d-Biocytin N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-yl]-1-oxopentyl]-L-lysine N6-[5-[(3aS,6aβ)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4α-yl]-1-oxopentyl]-L-lysine (2S)-6-[5-[(3aR,6S,6aS)-2-keto-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-6-yl]pentanoylamino]-2-amino-hexanoic acid (2S)-6-[5-[(3aR,6S,6aS)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-6-yl]pentanoylamino]-2-aminohexanoic acid (2S)-6-[5-[(3aR,6S,6aS)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-6-yl]pentanoylamino]-2-azanyl-hexanoic acid Biocytin 〔Nε-Biotinyl-L-lysine〕 Biocytin (&epsilon BIOCYTIN (Ε-BIOTINOYL-L-LYSINE);BCT;Nε-(+)-BIOTINYL-L-LYSINE -Biotinoyl-L-lysine) Nε H-Lys(biotinyl) L-Lys(biotinyl)-OH Nε-Biotinyl-L-lysine≥ 95% (NMR) Anti-BTD antibody produced in goat OTTHUMP00000160300 OTTHUMP00000209134 OTTHUMP00000209135 OTTHUMP00000209138 Biocytin *CAS 576-19-2* (2S)-6-{5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamido}-2-aminohexanoic acid L-Lysine, N6-[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]- Biocytin USP/EP/BP (2s)-6-[5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-2-aminohexanoic Acid 576-19-2 C16H28N4O4S Substrates by Enzyme Modified Amino Acids Enzymes, Inhibitors, and Substrates A - H Enzyme Substrates Biotinidase Biochemicals and Reagents Amino Acids and Peptides BioChemical Amino Acids & Derivatives Biotin Derivatives