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HEPES

CAS No.
7365-45-9
Chemical Name:
HEPES
Synonyms
HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
CBNumber:
CB5408557
Molecular Formula:
C8H18N2O4S
Molecular Weight:
238.3
MDL Number:
MFCD00006158
MOL File:
7365-45-9.mol
MSDS File:
SDS
Last updated:2023-10-23 17:13:05

HEPES Properties

Melting point 234-238 °C
Boiling point 408℃[at 101 325 Pa]
Density 1.07 g/mL at 20 °C
vapor pressure 0Pa at 25℃
refractive index n20/D 1.339
storage temp. 2-8°C
solubility H2O: 1 M at 20 °C, clear, colorless
form powder
color White
PH 5.0-6.5 (1Maqueous solution)
pka 7.5(at 25℃)
PH Range 7.0 - 7.6
Water Solubility Soluble
λmax λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck 14,4654
BRN 883043
Stability Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP -3.85 at 20℃
CAS DataBase Reference 7365-45-9(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII RWW266YE9I
EPA Substance Registry System 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319-H335
Precautionary statements  P261-P271-P280
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  24/25-22-36-26
WGK Germany  1
RTECS  TL6809000
TSCA  Yes
HS Code  29335995
NFPA 704
1
2 1

HEPES price More Price(151)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich PHG0001 HEPES 7365-45-9 1KIT $978 2024-03-01 Buy
Sigma-Aldrich H4034 HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture 7365-45-9 25KG $6140 2024-03-01 Buy
Sigma-Aldrich H3375 HEPES ≥99.5% (titration) 7365-45-9 25KG $5870 2024-03-01 Buy
Sigma-Aldrich H3375 HEPES ≥99.5% (titration) 7365-45-9 50KG $11060 2024-03-01 Buy
Sigma-Aldrich 83264 HEPES buffer solution 1?M in H2O 7365-45-9 100ML $67.1 2024-03-01 Buy
Product number Packaging Price Buy
PHG0001 1KIT $978 Buy
H4034 25KG $6140 Buy
H3375 25KG $5870 Buy
H3375 50KG $11060 Buy
83264 100ML $67.1 Buy

HEPES Chemical Properties,Uses,Production

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Uses

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

Uses

HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay. 

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

Multi purpose buffer used in biological research.

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

103-76-4
1562-00-1
7365-45-9
Synthesis of HEPES from 1-(2-Hydroxyethyl)piperazine and Sodium isethionate

HEPES Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from HEPES manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid pictures 2024-03-28 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
7365-45-9
US $0.00 / Kg/Drum 1KG 99% 200mt Jinan Finer Chemical Co., Ltd
HEPES pictures 2024-02-20 HEPES
7365-45-9
US $0.00 / kg 1kg 99.5% 1000tons Suzhou Genemill Biotechnology Co.,Ltd
HEPES-2- 4-(2-Hydroxyethyl)-1-piperazinyl ethanesulfonic Acid pictures 2024-02-20 HEPES-2- 4-(2-Hydroxyethyl)-1-piperazinyl ethanesulfonic Acid
7365-45-9
US $0.00 / kg 1kg 99.5% 1000tons Suzhou Genemill Biotechnology Co.,Ltd
  • HEPES pictures
  • HEPES
    7365-45-9
  • US $0.00 / kg
  • 99.5%
  • Suzhou Genemill Biotechnology Co.,Ltd
HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+% N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID)) Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz 1-HeptaneSulfonicAcidSod.SaltGr N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid) N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes) Hepes,LowSodium,FreeAcid HEPES free acid, DNase, Rnase and Protease free, for molecular biology, 99+% Hydroxyethyl)-1-piperazineethyanesulfonic acid, 4-(2- 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid(HEPES) 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid 4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid HEPES, for biochemistry, 99% 4-(2-Hydroxyethyl)-1-Piperazin Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- HEPES,BIOLOGICALBUFFER HEPES,BIOLOGICALBUFFER,ULTRAPURE N-(2-hydroxymethyl)piperazine-N`-(2-ethanesulfonic acid)(HEPES) N-2-HYDROXYETHYLPIPERAZINE-N-2-ETHANSULFONICACID(HEPES) 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid HEPES HEPES / 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULPHONIC ACID HEPES (4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID) HEPES ULTRAPURE N-2-HYDROXYETHYLPIPERAZINE-N''-2-ETHANE SULPHONIC ACID >99.5% N-[2-HYDROXYERHYL]PIPERAZINE-N''-ETHANESULFONICACID(HEPES) HEPES free acid ultrapure N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid, low sodium >99.5% 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research] HEPES(N-[2-Hydroxyethyl]piperazine-N'-[Ethanesulfonic acid]),Free Acid N'-2-N-2-hydroxyethyl piperazineethanesulfonic acid HEPES, >=99.5% (titration) HEPES SynonyMs 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid HEPES Buffer Solution (1M) HEPES,4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid HEPES Vetec(TM) reagent grade, 99.5% 4-CHLORO-1-NAPHTHOL ULTRA PURE GRADE POTASSIUM HYDROXIDE 0,05 MOL DUTY PAID POTASSIUM HYDROXIDE 0,5MOL/L DUTY PAID POTASSIUM HYDROXIDE 1 MOL/L DUTY PAID SELENIUM STAND MALATE DEHYDROGENASE ULTRA PURE GRADE PHOSPHOTUNGSTIC ACID HIGH PURITY GRADE 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci hepesfreeacid 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID LABOTEST-BB LT00138109 LABOTEST-BB LT00233156 HEPES HEPES-ACID HEPES BUFFERED SALINE HEPES BUFFER PH 7.5 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid hepes buffer (1M) aseptically filled*endotoxin te hepes free acid molecular biology*reagent HEPES N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)