BCIP/NBT
- CAS No.
- Chemical Name:
- BCIP/NBT
- Synonyms
- NBT/BCIP;BCIP/INT;BCIP/NBT;BM PURPLE;BCIP/NBT-BLUE;BCIP/NBT PLUS;NBT-X-PHOSPHATE;SIGMAFAST? BCIP?/NBT;NBT-BCIP(R) solution;INT/BCIP TOLUIDINE SALT
- CBNumber:
- CB5448175
- Molecular Formula:
- C48H34BrClN11O10P
- Molecular Weight:
- 1071.18
- MDL Number:
- MFCD00466954
- MOL File:
- Mol file
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305+P351+P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| WGK Germany | 2 |
BCIP/NBT price More Price(4)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | B1911 | BCIP®/NBT Liquid Substrate System ready to use solution | 100mL | $108 | 2024-03-01 | Buy | |
| Sigma-Aldrich | B5655 | SIGMAFAST™ BCIP®/NBT tablet | 5TABLETS | $58.3 | 2023-06-20 | Buy | |
| Sigma-Aldrich | B5655 | SIGMAFAST™ BCIP®/NBT tablet | 25TABLETS | $204 | 2023-06-20 | Buy | |
| American Custom Chemicals Corporation | HCH0127002 | 2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT 95.00% | 5MG | $499.85 | 2021-12-16 | Buy |
BCIP/NBT Chemical Properties,Uses,Production
Uses
SIGMAFAST BCIP?/NBT has been used as a substrate
- for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
- for alkaline phosphatase in the histochemical staining of primary osteoblasts
- for alkaline phosphatase staining in bone marrow stromal cells
Uses
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
General Description
BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.
Appearance prior to use: light yellow color;
After color development: dark purple
BCIP/NBT Preparation Products And Raw materials
Raw materials
Preparation Products
BCIP/NBT Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Aladdin Scientific | +1-833-552-7181 | sales@aladdinsci.com | United States | 57511 | 58 |
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
| Nantong Feiyu Biological Technology CO.,LTD | 0513-68819626; 13813788405 | feiyubio2@163.com | China | 4821 | 58 |
| Shanghai Yaji Biological Technology Co., Ltd. | 021-34661275 15301693058 | yajikit@163.com | China | 6633 | 58 |
| Shanghai Aladdin Biochemical Technology Co.,Ltd. | +86-18521732826 | market@aladdin-e.com | China | 48617 | 58 |
(BCIP/NBT)Related Search:
1of4