Maleimide Activated KLH
- CAS No.
- Chemical Name:
- Maleimide Activated KLH
- Synonyms
- Maleimide Activated KLH
- CBNumber:
- CB62897499
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Maleimide Activated KLH price More Price(1)
Maleimide Activated KLH Chemical Properties,Uses,Production
Uses
Maleimide-activated KLH (mKLH) has reactive maleimide groups on its surface, available for conjugation with a cysteine-containing peptide or a thiol-containing hapten. The reaction of the maleimide group and the thiol group proceeds rapidly and selectively under mild coupling conditions (pH 6.5-7.5) to yield a stable, covalently linked peptide-protein conjugate that may be used in immunization protocols or in antibody screening methods.
General Description
Maleimide Activated KLH is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated KLH contains approximately 160-320 maleimide groups for each KLH molecule. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellman′s reagent to detect unreacted thiol groups in the cysteine-containing peptide.
Maleimide Activated KLH Preparation Products And Raw materials
Raw materials
Preparation Products
Maleimide Activated KLH Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Beijing Boorsen Biotechnology Co., Ltd | 400-901-9800 18611424007 | cis@bioss.com.cn | China | 9518 | 58 |
Supplier | Advantage |
---|---|
Sigma-Aldrich | 80 |
Beijing Boorsen Biotechnology Co., Ltd | 58 |