Anti-Cathepsin B antibody, Mouse monoclonal

Uses

indirect ELISA, immunohistochemistry, In HCT-116 cells. 4-8μg/mL of antibody concentration may be used. A dilution of 1:800 in human pancreatic carcinoma cell line PANC-1 was used for immunoblotting

General Description

The CTSB (Cathepsin B) gene encoding cathepsin B is mapped to human chromosome 8p23.1. Cathepsin B are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell that take part in local proteolysis. Cathepsin B is both an endo- and exopeptidase, composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.

Biochem/physiol Actions

Cathepsins are lysosomal proteases activation of enzyme precursors, and tumor invasion and metastasis. It is involved in the proteolysis of many proteins involved in different physiological and pathological pathways. In the brain, this protease, also known as amyloid precursor protein secretase, is responsible for the proteolytic processing of amyloid precursor protein (APP). An incomplete proteolytic processing of APP has been found as a causative factor in Alzheimer disease. Furthermore, ablated genes coding cathepsin B and L leads to enormous neuronal cell death,probably due to autophagy abnormalities. Cathepsin B has been implicated in tumor progression by virtue of localization at the invading front of the tumor. For example, blocking cathepsin B expression in human glioblastoma SNB19 cells by a stable transfection of an expression vector expressing antisense cathepsin B, caused suppression of glioblastoma-induced neovascularization. Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W. Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules.

Raw materials

Preparation Products