Apoptotic DNA-Ladder Kit

CAS No.
Chemical Name:
Apoptotic DNA-Ladder Kit
Synonyms
Apoptotic DNA-Ladder Kit
CBNumber:
CB64669845
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Last updated:2022-08-15 17:36:37

Apoptotic DNA-Ladder Kit Properties

storage temp. 20-25°C

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictograms
GHS05,GHS07
Signal word  Danger
Hazard statements  H302-H315-H318-H412
Precautionary statements  P264-P270-P273-P280-P305+P351+P338+P310-P501

Apoptotic DNA-Ladder Kit Chemical Properties,Uses,Production

Uses

The Apoptotic DNA-Ladder Kit provides rapid isolation of DNA, which can be analyzed and characterized by gel electrophoresis for the determination of apoptotic cell death.

General Description

This kit provides a way to isolate apoptotic DNA fragments for DNA ladder analysis. The purification method used is much faster than other DNA purification methods (e.g., phenol/chloroform extraction, DNA precipitation). Purified DNA can be mixed directly with gel loading buffer and analyzed on an agarose gel.

The study of cell death involves characterizing mortality as apoptotic or necrotic. Apoptosis can be characterized by:

  • Prelytic, non-random fragmentation of DNA (“ladder” pattern after agarose-gel electrophoresis)1
  • Formation of membrane-bound vesicles (or “apoptotic bodies”)
  • Cell shrinkage due to a concentration of cytoplasm

Similarly, necrosis (or physiological cell death) is characterized by
  • Random digestion of DNA (DNA smear after agarose-gel electrophoresis)
  • Swollen organelles and cells, resulting from loss of membrane integrity and cell lysis
  • Postlytic DNA fragmentation

As these differences indicate, the classification of cell death can be accomplished through observing cell morphology, or less subjectively, by analyzing genomic DNA. Resolving the DNA on a gel provides a quick and documentable form of data to differentiate between apoptosis and necrosis.

Apoptotic DNA-Ladder Kit Preparation Products And Raw materials

Raw materials

Preparation Products

Apoptotic DNA-Ladder Kit Suppliers

Global( 18)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
Apoptotic DNA-Ladder Kit