AC-DEVD-AFC
- CAS No.
- 201608-14-2
- Chemical Name:
- AC-DEVD-AFC
- Synonyms
- AC-DEVD-AFC;AC-DEVD-AFC?, >98%;AC-DEVD-AFC USP/EP/BP;AC-ASP-GLU-VAL-ASP-AFC;CASPASE-3 SUBSTRATE (AFC);M.W. 729.62 C30H34F3N5O13;CASPASE-3 FLUOROGENIC SUBSTRATE;CASPASE-3 SUBSTRATE VII, FLUOROGENIC;FLUOROGENIC CASPASE 3 SUBSTRATE AC-DEVD-AFC;CASPASE 3 (APOPAIN) SUBSTRATE 1F, FLUOROGENIC
- CBNumber:
- CB7205880
- Molecular Formula:
- C30H34F3N5O13
- Molecular Weight:
- 729.61
- MDL Number:
- MFCD01310970
- MOL File:
- 201608-14-2.mol
- MSDS File:
- SDS
AC-DEVD-AFC price More Price(20)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | A0466 | N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin ≥90% (HPLC), powder | 201608-14-2 | 1mg | $203 | 2024-03-01 | Buy |
| Alfa Aesar | J64134 | N-Acetyl-Asp-Glu-Val-Asp-7-amino-4-(trifluoromethyl)coumarin | 201608-14-2 | 5mg | $350 | 2023-06-20 | Buy |
| Cayman Chemical | 14459 | Ac-DEVD-AFC ≥98% | 201608-14-2 | 1mg | $50 | 2024-03-01 | Buy |
| Cayman Chemical | 14459 | Ac-DEVD-AFC ≥98% | 201608-14-2 | 5mg | $184 | 2024-03-01 | Buy |
| Cayman Chemical | 14459 | Ac-DEVD-AFC ≥98% | 201608-14-2 | 10mg | $341 | 2024-03-01 | Buy |
AC-DEVD-AFC Chemical Properties,Uses,Production
Uses
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes'' shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.
Biological Activity
ac-devd-afc is a fluorogenic substrate for activated caspase-3 (cpp32) with km value of 9.7 μm, and related cysteine proteases [1][2].apoptosis is a process of programmed cell death that occurs in multicellular organisms. caspase are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation. caspase activation is a major event in apoptosis. caspase-3 cleaves and activates caspases 6 and 7, and is processed and activated by caspases 8, 9, and 10 [1][2][3].ac-devd-afc (n-acetyl-asp-glu-val-asp-7-amido-4-trifluoromethylcoumarin) is a fluorogenic substrate for activated caspase-3 and related caspases. during apoptosis, activated caspase-3 cleaves poly (adp-ribose) polymerase, which it specifically targets at the amino sequence asp-glu-val-asp (devd). sequence is based on parp cleavage at asp216 for caspase-3 [1][2]. caspase activity can be quantified by fluorescent detection of free afc (7-amino-4-trifluoromethylcoumarin) at excitation 400 nm and emission 505 nm [2].
Biochem/physiol Actions
A fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. The 7-amido-4-trifluoromethylcoumarin derivative has better membrane permeability than the 7-amido-4-methylcoumarin derivative (A1086).
References
[1]. lazebnik ya1, kaufmann sh, desnoyers s, et al. cleavage of poly(adp-ribose) polymerase by a proteinase with properties like ice. nature. 1994 sep 22;371(6495):346-7.
[2]. xiang, j.,chao, d.t., and korsmeyer, s.j. bax-induced cell death may not require interleukin 1β-converting enzyme-like proteases. proceedings of the national academy of sciences of the united states of america 93, 14559-14563 (1996).
AC-DEVD-AFC Preparation Products And Raw materials
Raw materials
Preparation Products
AC-DEVD-AFC Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shenzhen Nexconn Pharmatechs Ltd | +86-755-89396905 +86-15013857715 | admin@nexconn.com | China | 10248 | 58 |
| Hubei Jusheng Technology Co.,Ltd. | 18871490254 | linda@hubeijusheng.com | CHINA | 28180 | 58 |
| Career Henan Chemica Co | +86-0371-86658258 15093356674; | laboratory@coreychem.com | China | 30255 | 58 |
| Dideu Industries Group Limited | +86-29-89586680 +86-15129568250 | 1026@dideu.com | China | 29220 | 58 |
| BOC Sciences | +16314854226 | inquiry@bocsci.com | United States | 19743 | 58 |
| Zhejiang J&C Biological Technology Co.,Limited | +1-2135480471 +1-2135480471 | sales@sarms4muscle.com | China | 10523 | 58 |
| Chengdu Youngshe Chemical Co., Ltd. | +8618108235634 | Cecilia@youngshechem.com | China | 2345 | 58 |
| Nanjing TGpeptide | +86-13347807150 +86-13347807150 | support@tgpeptide.com | China | 3279 | 58 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 821-50328103-801 18930552037 | 3bsc@sina.com | China | 15848 | 69 |
| GL Biochem (Shanghai) Ltd | 21-61263452 13641803416 | ymbetter@glbiochem.com | China | 9981 | 64 |