KAPA2G Robust PCR Kit

Uses

KAPA2G Robust PCR Kit has been used for:

• Amplification of GC- or AT-rich templates and gene amplification
• Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
• Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
• Colony PCR
• Routine PCR
• PCR amplification
• Avipoxvirus specific PCR
• Simplex PCR
• Semi-quantitative PCR

Uses

KAPA2G Robust PCR Kit is suitable for:

• Amplification of GC- or AT-rich templates
• Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
• Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
• Colony PCR
• Polymerase chain reaction

General Description

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

Biochem/physiol Actions

KAPA2G Robust DNA polymerase has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Robust is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. KAPA2G Robust is a highly processive DNA polymerase, capable of amplifying DNA fragments of up to 1 kb in size with a 15 sec/cycle extension time. The use of excessive extension times is likely to result in smearing and nonspecific amplification. For amplicons <1 kb with genomic DNA as a template, 15 sec per cycle should be sufficient for a high yield of the product. In the case of longer amplicons, the time may be increased to a maximum of 60 sec/kb, in 15-sec increments. PCR products generated by KAPA2G Robust are 3′-dA-tailed and may be cloned into TA cloning vectors.