Transcriptor One-Step RT-PCR Kit

Uses

Transcriptor One-Step RT-PCR Kit has been used in the amplification of:

• hepatitis C virus (HCV) fragments
• heavy chain CH3 region and light chain polyA region
• murine leukemia virus (MLV)

General Description

One-step RT-PCR is a very sensitive technique for determining the presence or absence of RNA templates, or to quantify the levels of expression through qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels (e.g., for virus-level quantification). This RT-PCR method also facilitates the amplification of rare messages for cloning. The one-step format is convenient, shows reduced reaction-to-reaction variability, and prevents contamination by minimizing hands-on steps.
The Transcriptor One-Step RT-PCR Kit offers the outstanding benefits of Transcriptor Reverse Transcriptase, as well as the advantages of a hot start one-step RT-PCR format.