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2′,7′-Dichlorodihydrofluorescein Diacetate

2′,7′-Dichlorodihydrofluorescein Diacetate Structure
2′,7′-Dichlorodihydrofluorescein Diacetate structure
CAS No.
4091-99-0
Chemical Name:
2′,7′-Dichlorodihydrofluorescein Diacetate
Synonyms
DCFH-DA;DCFH;Dichlorofluorescin diacetate;187263;DCFH | DCFH-DA;H2DCFDA / DCFH-DA;2'',7''-Dichlorofluorescein diac;2',7'-Dichlorofluorescin diacetat;-Dichlorodihydrofluorescein diacetate;2' 7'-DICHLOROFLUORESCIN DIACETATE FOR&
CBNumber:
CB8497619
Molecular Formula:
C24H16Cl2O7
Molecular Weight:
487.29
MDL Number:
MFCD00128955
MOL File:
4091-99-0.mol
MSDS File:
SDS
Last updated:2023-09-28 17:55:30
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2′,7′-Dichlorodihydrofluorescein Diacetate Properties

Melting point 209-210 °C(lit.)
Boiling point 593.5±50.0 °C(Predicted)
Density 1.463±0.06 g/cm3(Predicted)
storage temp. -20°C
solubility DMF: soluble
form Crystalline solid
pka 3.79±0.36(Predicted)
BRN 4610273
Stability Light Sensitive
EPA Substance Registry System 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319
Precautionary statements  P501-P270-P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313-P301+P312+P330
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  22-24/25-26
WGK Germany  3
F  10-21
HS Code  29329990
NFPA 704
0
2 0

2′,7′-Dichlorodihydrofluorescein Diacetate price More Price(29)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich D6883 2′,7′-Dichlorofluorescin diacetate ≥97% 4091-99-0 50mg $53.6 2024-03-01 Buy
Sigma-Aldrich 287810 2?,7?-Dichlorofluorescin Diacetate 4091-99-0 100mg $107 2024-03-01 Buy
Cayman Chemical 85155 2,7-Dichlorodihydrofluorescein diacetate ≥95% 4091-99-0 50mg $33 2024-03-01 Buy
Cayman Chemical 85155 2,7-Dichlorodihydrofluorescein diacetate ≥95% 4091-99-0 100mg $62 2024-03-01 Buy
Sigma-Aldrich D6883 2′,7′-Dichlorofluorescin diacetate ≥97% 4091-99-0 250mg $190 2024-03-01 Buy
Product number Packaging Price Buy
D6883 50mg $53.6 Buy
287810 100mg $107 Buy
85155 50mg $33 Buy
85155 100mg $62 Buy
D6883 250mg $190 Buy

2′,7′-Dichlorodihydrofluorescein Diacetate Chemical Properties,Uses,Production

Description

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1).

Chemical Properties

white to yellow-beige or pale pink powder

Uses

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

Uses

2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.

Uses

Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.

Biochem/physiol Actions

Cell permeable: yes

storage

Store at -20°C

2′,7′-Dichlorodihydrofluorescein Diacetate Preparation Products And Raw materials

Raw materials

2',7'-DICHLOROFLUORESCIN DIACETATE

Preparation Products

2',7'-Dichlorofluorescein 2',7'-dichlorodihydrofluorescein

2′,7′-Dichlorodihydrofluorescein Diacetate Suppliers

Global( 167)Suppliers
Supplier Tel Email Country ProdList Advantage
Hebei Mojin Biotechnology Co., Ltd 		+8613288715578 sales@hbmojin.com China 12456 58
Henan Tianfu Chemical Co.,Ltd. 		+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21691 55
ATK CHEMICAL COMPANY LIMITED 		+undefined-21-51877795 ivan@atkchemical.com China 32480 60
CONIER CHEM AND PHARMA LIMITED 		+8618523575427 sales@conier.com China 49390 58
career henan chemical co 		+86-0371-86658258 15093356674; factory@coreychem.com China 29826 58
TargetMol Chemicals Inc. 		+1-781-999-5354 +1-00000000000 marketing@targetmol.com United States 19892 58
Hubei Ipure Biology Co., Ltd 		+8613367258412 ada@ipurechemical.com China 10326 58
Hefei TNJ Chemical Industry Co.,Ltd. 		0551-65418671 sales@tnjchem.com China 34572 58
HONG KONG IPURE BIOLOGY CO.,LIMITED 		86 18062405514 18062405514 ada@ipurechemical.com CHINA 3465 58
Xi'an MC Biotech, Co., Ltd. 		029-89275612 +8618991951683 mcbio_sales@163.com China 2255 58
Supplier Advantage
Hebei Mojin Biotechnology Co., Ltd 		58
Henan Tianfu Chemical Co.,Ltd. 		55
ATK CHEMICAL COMPANY LIMITED 		60
CONIER CHEM AND PHARMA LIMITED 		58
career henan chemical co 		58
TargetMol Chemicals Inc. 		58
Hubei Ipure Biology Co., Ltd 		58
Hefei TNJ Chemical Industry Co.,Ltd. 		58
HONG KONG IPURE BIOLOGY CO.,LIMITED 		58
Xi'an MC Biotech, Co., Ltd. 		58

View Lastest Price from 2′,7′-Dichlorodihydrofluorescein Diacetate manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
2′,7′-Dichlorodihydrofluorescein Diacetate pictures 2023-09-28 2′,7′-Dichlorodihydrofluorescein Diacetate
4091-99-0
 US $0.00 / KG 1KG 99% 50000KG/month Hebei Mojin Biotechnology Co., Ltd
2′,7′-Dichlorodihydrofluorescein Diacetate pictures 2019-12-31 2′,7′-Dichlorodihydrofluorescein Diacetate
4091-99-0
 US $1.00 / KG 1KG 95-99% 1ton Career Henan Chemical Co

2′,7′-Dichlorodihydrofluorescein Diacetate Spectrum

2′,7′-Dichlorodihydrofluorescein Diacetate(4091-99-0)1HNMR

4091-99-0(2′,7′-Dichlorodihydrofluorescein Diacetate)Related Search:

Adenine Sulfate Dihydrate 2',7'-Dichlorofluorescein 2′,7′-Dichlorodihydrofluorescein Diacetate 2',7'-DICHLOROFLUORESCIN DIACETATE 5-(AND-6)-CARBOXY-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE
5-(and-6)-carboxy-2`,7`-dichlorofluorescein diacetate 6-Carboxy-2',7'-dichlorofluorescein 3',6'-Diacetate SucciniMidyl Ester 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 2'',7''-DICHLOROFLUORESCIN DIACETATE 2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE (2',7'-DICHLOROFLUORESCIN DIACETATE; H2DCFDA)
5-CARBOXY-2'',7''-DICHLORODIHYDROFLUORESCEIN DIACETATE, DI(ACETOXYMETHYL ESTER) 5-(AND-6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE, SUCCINIMIDYL ESTER 6-CARBOXY-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE, DI(ACETOXYMETHYL ESTER) 6-CARBOXY-2'',7''-DICHLORODIHYDROFLUORESCIN DIACETATE, DI(ACETOXYMETHYL ESTER) CALCIUM GREEN(TM)-2, AM
5-(AND-6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE 5-(AND-6)-CHLOROMETHYL-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE 2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE, SUCCINIMIDYL ESTER

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2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate 2',7'-DICHLOROFLUORESCIN DIACETATE, FOR FLUORESCENCE 2',7'-Dichlorodihydrofluoresceinediacetate 2'',7''-Dichlorofluorescein diac 2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid 2-(3,6-diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid 2',7'-DICHLOROFLUORESCIN DI-ACETATE ANALYTICAL GRADE 2′,7′-Dichlorofluorescin diacetate,2′,7′-Dichlorodihydrofluorescein diacetate 2',7'-Dichlorofluorescin diacetat o-(2,7-Dichloro-3,6-dihydroxyxanthen-9-yl)benzoic Acid Diacetate Dichlorofluorescin diacetate analytical grade H2DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] H2DCFDA [2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE];DCFH-DA 2-(3,6-Diacetoxy-2,7-dichloro-9H-xanthen-9-yl) -Dichlorodihydrofluorescein diacetate 2' 7'-DICHLOROFLUORESCIN DIACETATE FOR& 2-[3,6-bis(acetyloxy)-2,7-dichloro-9h-xanthen-9-yl]-benzoicaci Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]- H2DCFDA[2',7'-DICHLORODIHYDROFLUORESCEINDIACETATChemicalbookE] DCFH | DCFH-DA H2DCFDA / DCFH-DA 2′,7′-Dichlorofluorescin diacetate, ≥97% 2,7-Dichlorofluorescein Diacetate (DCFH) extrapure, 97% 187263 2,7-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem 2',7'-Dichlorodihydrofluorescein diacetate [2',7'-Dichlorofluorescin diacetate] *CAS 4091-99-0* Dichlorofluorescin diacetate DCFH-DA DCFH 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoicaci 2',7'-Dichlorodihydrofluorescein diacetate 4091-99-0 C24H16Cl2O7