Monoclonal Anti-Digoxin

Uses

Monoclonal Anti-Digoxin-FITC antibody produced in mouse has been used in immuno fluorescence in situ hybridization?(FISH) and FISH.

Uses

Monoclonal Anti-Digoxin antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• dot blot
• flow cytometry
• fluorescence in situ hybridization (FISH)
• DNA hybridization
• in-situ hybridization (ISH)
• immunodetection of the probe and chromosome X

General Description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier.

Biochem/physiol Actions

Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.