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Cholic acid

Chemical properties Uses Identification test Analysis of the content Toxicity Production methods
Cholic acid
Cholic acid structure
Chemical Name:
Cholic acid
Molecular Formula:
Formula Weight:
MOL File:

Cholic acid Properties

Melting point:
200-201 °C (lit.)
Boiling point:
449.08°C (rough estimate)
36 º (c=0.6, 95% EtOH)
1.0310 (rough estimate)
refractive index 
1.5800 (estimate)
Flash point:
storage temp. 
methanol: 0.1 g/mL, clear
4.98(at 20℃)
Water Solubility 
0.28 g/L (15 ºC)
Substances Added to Food (formerly EAFUS)
SCOGS (Select Committee on GRAS Substances)
Cholic acid
CAS DataBase Reference
81-25-4(CAS DataBase Reference)
EWG's Food Scores
NIST Chemistry Reference
Cholic acid(81-25-4)
ATC code
EPA Substance Registry System
Cholan-24-oic acid, 3,7,12-trihydroxy-, (3.alpha.,5.beta.,7.alpha.,12.alpha.)- (81-25-4)
  • Risk and Safety Statements
Signal word  Danger
Hazard statements  H301+H311+H331-H370-H315-H319
Precautionary statements  P261-P280-P302+P352+P312-P304+P340+P312-P403+P233-P305+P351+P338
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  24/25-36-26
RIDADR  UN1230 - class 3 - PG 2 - Methanol, solution
WGK Germany  2
RTECS  FZ9350000
HS Code  29181990

Cholic acid price More Price(48)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich C-146 Cholic acid 500?μg/mL in methanol, certified reference material, Cerilliant? 81-25-4 1 mL $69.9 2021-12-16 Buy
Sigma-Aldrich C2158000 Cholic acid European Pharmacopoeia (EP) Reference Standard 81-25-4 $190 2021-12-16 Buy
Sigma-Aldrich 1133503 Cholic acid United States Pharmacopeia (USP) Reference Standard 81-25-4 2g $353 2021-12-16 Buy
Sigma-Aldrich C1129 Cholic acid from bovine and/or ovine, ≥98% 81-25-4 1kg $668 2021-12-16 Buy
Alfa Aesar A11257 Cholic acid, 98+% 81-25-4 100g $98.5 2021-12-16 Buy

Cholic acid Chemical Properties,Uses,Production

Chemical properties

This agent exist in the bile cattle, sheep, pig. It is a colorless sheet or white crystalline powder. Some have a bitter to sweet taste. Its melting point is 198℃, specific rotation is (c = 0.6, ethanol) +37°. 1g cholic acid dissolved in about 300ml ethanol or acetone, 7ml glacial acetic acid. A small amount of cholic acid is soluble in water. The monohydrate was a white flake crystal. In 1927, H.Wieland (Germany) research accomplished bile acid composition, and won the Nobel Prize in chemistry.


(1) Emulsifiers.
(2) Used for biochemical research, as pharmaceutical intermediates. Sodium cholate is a cholagogue, for the treatment of cholecystitis, bile deficiency, intestinal indigestion embolism.
(3) As organic acids with steroid structure, cholic acid can emulsify fat, promote its digestion.
(4) Non-denaturing ionic detergents for the extraction of membrane proteins.

Identification test

Solubility: hardly insoluble in water; soluble in ethanol. According to OT-42 method.
The melting range : 197~202 ℃. As determined by conventional methods.
Add 50% acetic acid solution to prepare 0.02% of the sample solution; take 1ml, 1% furfural solution, 6ml of water and concentrated sulfuric acid 5ml. This mixture should be converted into rosiness in 5min and then turn purple.
Take about 10mg sample, add 2 drops of benzaldehyde and 3: 1 sulfuric acid 3 drops, heating at 50 ℃ for 5min. Plus glacial acetic acid about 10ml, then it should be brown.

Analysis of the content

Weigh about 400mg of the sample accurately; dried at 140 ℃ for 4h; put it into a 250ml flask; add 20ml of water and 40ml of ethanol; cover the surface dish, and gently heat to dissolve and cool in the steam bath. Add 5 drops of phenolphthalein test solution (TS-167), titrate with 0.1mol/L sodium hydroxide solution to pink and keep 15s, and make the necessary correction with the same time blank test. Each 1ml of 0.1 mol/L sodium hydroxide corresponds to 40.86 mg of cholic acid (C24 H40O5).


ADI O~1.25mg/kg(FAO/WH0,2001)。
ADI O~1.25 mg/kg (FAO/WH0, 2001)。

Production methods

(1) Extracting from livestock (pigs, cattle, sheep, rabbits) the bile.
1.Ethanol crystallization method
Preparation of crude cholic acid of cattle, sheep: take bovine or sheep bile; add 100 g/L sodium hydroxide; heat to boil for 12-18h to get saponification solution. Cooling. Adding acid to pH 1, precipitating cholic acid, removing bile acid, undergoing boiling and rinsing, drying at 75℃, milling can obtain bovine cholic acid.
sodium cholate[NaOH] → [100 ℃, 12-18h] saponified solution[H2SO4] → [pH1, 75 ℃] crude cholic acid of cattle, sheep.
Preparation of sodium cholate: add 0.5-1 times of crude cholic acid to 95% ethanol, and dissolve the solids by heating reflux method; cool. Broke the crystallization; filter; add 95% washing ethanol to make the filtrate colorless. Crystallize by adding 4 times the amount of ethanol; add 100-150g/L of activated carbon; dissolve the solids by heating reflux method; filter the liquid when it is hot. The filtrate concentrated to the original volume of 1/4. Through cooling, crystallization, filtration, adding ethanol to wash the crystallization can obtain the sodium cholate products.
Crude cholic acid cattle, sheep [ethanol, activated carbon] → refined liquid [90 ℃ below] → sodium cholate products
2.  Ethyl acetate separation method
Preparation of crude hyocholic acid: Add 3-3.5 times of saturated lime supernatant into fresh pig bile under stirring, then continue to stir it for 5-10min. heating to boiling for 2min, cooling, through filtration, adding hydrochloric acid to PH3.5 to get precipitation, standing for more than 12h can obtain crude acid. Removing, washing, adding 1.5 times the sodium hydroxide, plus 9 times the water, heating and boiling 12-18h, cooling, standing overnight obtain paste. Add water and sulfuric acid to pH 1 to precipitate pig cholic acid. Removing, crushing, rinsing to no acidity, through filtration gains crude pig cholic acid.
[pig bile] [saturated limewater] →[100℃, pH 11-12] Basic filtrate [HCl] → [pH3.5] Crude cholic acid [water, NaOH] → paste [H2SO4] → [pH1] crude pig cholic acid
Preparation of pig cholic acid products: add 4 times the amount of ethyl acetate to the crude pig cholic acid. Add 150-200g/L activated carbon; heat and flux for 0.5h; cool; filter; add 1.5-2.5 times ethyl acetate to filter cake; combine the filtrates twice. Adding 200g/L anhydrous sodium sulfate, standing overnight, concentrated to the original volume of 1/3, releasing, cooling crystallization, through filtration, washing with ethyl acetate crystallization, drying can obtain pig cholic acid products.
Crude pig cholic acid [ethyl acetate, activated carbon] → filtrate [anhydrous sodium sulfate] → filtrate [concentration] → pig cholic acid products.

Chemical Properties

white to light beige crystalline powder


Choleretic produced by, and isolated from liver cells.


asthma therapeutic


Cholic Acid is an emulsifier that exists as colorless plates or a white crystalline powder which has a bitter taste with a sweetish aftertaste. it is slightly soluble in water. it functions as an emulsifying agent in egg white.


ChEBI: A bile acid that is 5beta-cholan-24-oic acid bearing three alpha-hydroxy substituents at position 3, 7 and 12.

General Description

This Certified Spiking Solution? is suitable as a starting material in preparation of linearity standards, calibrators, and controls for use in LC-MS/MS and GC/MS bile acid testing methods. Cholic acid is a primary bile acid that plays an important role in cholesterol homeostasis and intestinal absorption of dietary vitamins and lipids. Levels of cholic acid can serve as markers for inborn and acquired hepatobiliary disorders such as biliary atresia and familial intrahepatic cholestasis as well as inborn errors of bile acid synthesis and sclerosing cholangitis.

Purification Methods

This bile acid crystallises from H2O or wet Et2O (as hydrate) or EtOH (as alcoholate). Dry it under vacuum at 94o. When an alcoholic solution of cholic acid + I2 is added to aqueous KI, it forms a molecular compound (C24H40O5I)4 KI. H2O. The methyl ester is dimorphic with m 155o and 162o and [] D 20 +25o (EtOH). [Anderson et al. Biochem J 67 323 1957, 85 236 1962, Beilstein 10 III 2162, IV 2071.]

Cholic acid Preparation Products And Raw materials

Raw materials

Preparation Products

Cholic acid Suppliers

Global( 442)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Hubei Ipure Biology Co., Ltd
18062427325; China 10352 58
Frapp's ChemicalNFTZ Co., Ltd.
+86 (576) 8169-6106
+86 (576) 8169-6105 China 886 50
Capot Chemical Co.,Ltd.
+86(0)13336195806 +86-571-85586718
+86-571-85864795 China 20012 60
Henan Tianfu Chemical Co.,Ltd.
0371-55170693 China 22607 55
Hangzhou FandaChem Co.,Ltd.
+86-571-56059825 CHINA 9134 55
Shanghai Zheyan Biotech Co., Ltd.
18017610038 CHINA 3623 58
career henan chemical co
+86-0371-55982848 China 29954 58
Jinan Chenghui-Shuangda Chemical Co.,Ltd
+86-531-58897093 CHINA 158 58
Chengdu GLP biotechnology Co Ltd
13350802083 028-87075086 CHINA 1824 58
Chengdu Biopurify Phytochemicals Ltd.
18482058008 18080483897 China 2712 58

View Lastest Price from Cholic acid manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2021-12-01 Cholic Acid
US $9.00 / KG 1KG 99% 10 ton Hebei Crovell Biotech Co Ltd
2021-11-30 Cholic acid
US $350.00 / KG 10Kg/Bag 98% 1000KG Baoji Guokang Bio-Technology Co., Ltd.
2021-11-02 Cholic acid
US $285.00 / KG 1KG 98% 1000kg Baoji Guokang Healthchem co.,ltd

Cholic acid Spectrum

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