Duolink In Situ Detection Reagents Brightfield

CAS No.
Chemical Name:
Duolink In Situ Detection Reagents Brightfield
Synonyms
Duolink? In Situ Detection Reagents Brightfield
CBNumber:
CB92839148
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Last updated:2022-08-15 17:41:03

Duolink In Situ Detection Reagents Brightfield Properties

storage temp. -20°C

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictogramsGHS hazard pictogramsGHS hazard pictograms
GHS02,GHS05,GHS06,GHS08
Signal word  Danger
Hazard statements  H225-H302+H312-H315-H317-H318-H331-H341-H412
Precautionary statements  P210-P273-P280-P303+P361+P353-P304+P340+P311-P305+P351+P338

Duolink In Situ Detection Reagents Brightfield price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich DUO92012 Duolink® In Situ Detection Reagents Brightfield 30REACTIONS $655 2023-06-20 Buy
Sigma-Aldrich DUO92012 Duolink® In Situ Detection Reagents Brightfield 100REACTIONS $970 2023-06-20 Buy
Product number Packaging Price Buy
DUO92012 30REACTIONS $655 Buy
DUO92012 100REACTIONS $970 Buy

Duolink In Situ Detection Reagents Brightfield Chemical Properties,Uses,Production

Uses

Duolink?proximity ligation assay(PLA?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink? In Situ Brightfield Protocol to use this product.

Visit our Duolink? PLA Resource Center for information on how to run a Duolink? experiment, applications, troubleshooting, and more.

To perform a complete Duolink? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink? PLA probes. Analysis is carried out using HRP for brightfield detection.

Specificity
Brightfield detection reagents are used for samples with significant autofluorescence.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink? in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink? projects

View full Duolink? product list

Duolink In Situ Detection Reagents Brightfield Preparation Products And Raw materials

Raw materials

Preparation Products

Duolink In Situ Detection Reagents Brightfield Suppliers

Global( 2)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
Duolink? In Situ Detection Reagents Brightfield