PCR DIG Probe Synthesis Kit

CAS No.
Chemical Name:
PCR DIG Probe Synthesis Kit
Synonyms
PCR DIG Probe Synthesis Kit
CBNumber:
CB94670523
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Last updated:2022-08-15 17:36:37

PCR DIG Probe Synthesis Kit Properties

storage temp. -20°C

SAFETY

Risk and Safety Statements

Hazard statements  H412
Precautionary statements  P273-P501

PCR DIG Probe Synthesis Kit price More Price(1)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 11636090910 PCR DIG Probe Synthesis Kit sufficient for 25?reaction (50 μL final reaction volume) 25REACTIONS $590 2024-03-01 Buy
Product number Packaging Price Buy
11636090910 25REACTIONS $590 Buy

PCR DIG Probe Synthesis Kit Chemical Properties,Uses,Production

Uses

Digoxigenin (DIG)-labeled DNA probes produced from the PCR DIG Probe Synthesis Kit is suitable for low-(single)-copy gene detection of rare mRNA in Southern and northern blots. DIG-labeled DNA probes have also been used for labeling of DNA during in situ hybridization.
The concentration of the supplied dUTP-nucleotide mix can be adjusted according to probe length. Labeling effectiveness can quickly be determined on an agarose gel.
Stripping and reprobing of membranes is possible multiple times following the protocol in the package insert.
One PCR labeling reaction (50 μl) will typically yield enough probe for 20 ml hybridization solution. The kit can be used for approximately 25 reactions (50 μl).

General Description

The PCR DIG Probe Synthesis Kit contains an alkali-labile digoxigenin (DIG)-11deoxyuridinetriphosphate (dUTP) formulation. This kit is for convenient and efficient generation of DIG-labeled DNA probes that are highly sensitive in polymerase chain reaction (PCR). The probes are labeled with DIG-11dUTP (alkali-labile), by the method of PCR. The ratio of DIG-dUTP:dTTP is 1:2.
The PCR DIG Probe Synthesis Kit contains the Expand High Fidelity DNA polymerase mix. This robust enzyme mix with proofreading activity will polymerize probes 40 bp to 5 kb long using 10 pg plasmid DNA and 10 ng genomic DNA as template. The DIG-labeled probes are stable for over one year.

PCR DIG Probe Synthesis Kit Preparation Products And Raw materials

Raw materials

Preparation Products

PCR DIG Probe Synthesis Kit Suppliers

Global( 1)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
PCR DIG Probe Synthesis Kit