β-Gal ELISA

CAS No.
Chemical Name:
β-Gal ELISA
Synonyms
β-Gal ELISA
CBNumber:
CB94670679
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file

β-Gal ELISA Properties

storage temp. 2-8°C

β-Gal ELISA Chemical Properties,Uses,Production

Uses

For research use only. Not for use in diagnostic procedures.
The β-Gal ELISA is used to quantitatively measure β-Gal expression in eukaryotic cells transfected with a plasmid bearing a β-Gal-encoding reporter gene. The β-Gal ELISA may also be used for quantification of fusion proteins, produced by in-frame cloning into an appropriate β-Gal encoding DNA construct.

General Description

β-Gal (β-D-galactoside galactohydrolase, EC 3.2.1.23) from E. coli is specifically detected by the β-Gal ELISA. The β-Gal enzyme from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.

Contents:

  • β-Gal Enzyme (from E. coli)
  • Anti-β-Gal-digoxigenin
  • Anti-DIG-peroxidase
  • POD Substrate
  • Substrate Enhancer
  • Washing Buffer concentrate, 10x
  • Sample Buffer
  • Lysis Buffer concentrate, 5x
  • Two Microplates, strip frame with 12 modules of 8 wells, precoated with anti-β-Gal
  • Self-adhesive Plate Cover Foils (3)

Biochem/physiol Actions

Promoter activity in transfected mammalian cells is generally studied by linking the promoter sequence to a gene that encodes an easily detectable “reporter” protein, such as chloramphenicol acetyltransferase (CAT), β-galactosidase (β-Gal), or luciferase (Luc).The E. coli lacZ gene, encoding the enzyme β-galactosidase (β-Gal), has become one of the standard reporter genes used in transfection experiments. Traditionally, β-Gal activity is measured using colorimetric assays with o-nitrophenol-β-D-galactopyranoside (ONPG) or chlorophenol red β-D-galactopyranoside (CPRG) as substrates. These assays are not sensitive enough to detect small amounts of the enzyme. Another disadvantage is that in order to enable discrimination between the endogenous lysosomal β-Gal activity and the bacterial enzyme, the colorimetric assays must be performed at an alkaline pH. In contrast, β-Gal ELISA specifically detects the bacterial enzyme, but not the analogous lysosomal β-galactosidase.

β-Gal ELISA Preparation Products And Raw materials

Raw materials

Preparation Products

β-Gal ELISA Suppliers

Global( 1)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
β-Gal ELISA