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EC 3.1.3.1

EC 3.1.3.1
EC 3.1.3.1 structure
CAS No.
9001-78-9
Chemical Name:
EC 3.1.3.1
Synonyms
AP;CAP;AKP;CIAP;MCAP;IRAP;HUNK1;HUNKI;IL1F3;Ostase
CBNumber:
CB9690084
Molecular Formula:
C21H36N8O6
Formula Weight:
496.56054
MOL File:
9001-78-9.mol

EC 3.1.3.1 Properties

Density 
1.12 g/mL at 20 °C
storage temp. 
2-8°C
form 
suspension
color 
white
Specific Gravity
1
Water Solubility 
It is soluble in water.
Stability:
Stability Incompatible with strong oxidizing agents

SAFETY

Hazard Codes  B,Xi
Risk Statements  36/37/38
Safety Statements  26-36-24/25-22-23
WGK Germany  3
3-10
TSCA  Yes
HS Code  35079090

EC 3.1.3.1 price More Price(43)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 524572 Phosphatase, Alkaline, Calf Intestine 9001-78-9 1ku $59 2018-11-20 Buy
Sigma-Aldrich 524545 Phosphatase, Alkaline, 9001-78-9 100u $630 2018-11-20 Buy
Alfa Aesar J61037 Alkaline Phosphatase, calf intestine, EIA Grade 9001-78-9 1mg $122 2018-11-13 Buy
Alfa Aesar J61037 Alkaline Phosphatase, calf intestine, EIA Grade 9001-78-9 5mg $514 2018-11-13 Buy
Sigma-Aldrich P7640 Phosphatase, Alkaline from bovine intestinal mucosa lyophilized powder, ≥10DEA units/mg solid 9001-78-9 5g $627 2018-11-20 Buy

EC 3.1.3.1 Chemical Properties,Uses,Production

Chemical Properties

solid

Uses

Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.

Uses

Commonly used to remove the 5′-terminal phosphate from nucleic acids.

Purification Methods

The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]

EC 3.1.3.1 Preparation Products And Raw materials

Raw materials

Preparation Products


EC 3.1.3.1 Suppliers

Global( 107)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Henan DaKen Chemical CO.,LTD.
+86-371-55531817
info@dakenchem.com CHINA 21922 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com CHINA 20680 55
Hefei Bomei Biotechnology Co., Ltd. 13739298932
531626407@qq.com CHINA 2966 58
J & K SCIENTIFIC LTD. 400-666-7788 +86-10-82848833
+86-10-82849933 jkinfo@jkchemical.com;market6@jkchemical.com China 96815 76
Meryer (Shanghai) Chemical Technology Co., Ltd. +86-(0)21-61259100(Shanghai) +86-(0)755-86170099(ShenZhen) +86-(0)10-62670440(Beijing)
+86-(0)21-61259102(Shanghai) +86-(0)755-86170066(ShenZhen) +86-(0)10-88580358(Beijing) sh@meryer.com China 40277 62
Energy Chemical 021-58432009 / 400-005-6266
021-58436166-800 info@energy-chemical.com China 44046 61
Shanghai Longsheng chemical Co.,Ltd. 021-58099637,58099652,58099650,58099609 To 8009,8068; 13585536065,QQ:975986869
0086-21-33191781 sales@shlschem.com; bin.wu@shlschem.com;xianjun.hong@shlschem.com China 9824 59
Chengdu XiYa Chemical Technology Co., Ltd. 4008-626-111
028-84752058 sale@xiyashiji.com China 9636 57
Chengdu Chuanke Fine Chemicals C.,Ltd. +86 (28) 68626087 ;+86-28-85405627
+86-28-85408898 customertwo@ckfc.asia China 28 62
Tianjin heowns Biochemical Technology Co., Ltd. 400 638 7771
sales@heowns.com China 14464 57

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