CLOSTRIPAIN
- CAS No.
- 9028-00-6
- Chemical Name:
- CLOSTRIPAIN
- Synonyms
- PROTEINASE;CLOSTRIPAIN;EC 3.4.22.8;IUB: 3.4.22.8;E.C. 3.4.4.20;alpha-Clostripain;ENDOPROTEINASE-ARG-C;CLOSTRIDIOPEPTIDASE B;clostripain from clost. histolyticum;Clostridium histolyticum proteinase B
- CBNumber:
- CB9768318
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MFCD00130814
- MOL File:
- Mol file
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS07,GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H315-H319-H334-H335 |
| Precautionary statements | P261-P264-P271-P302+P352-P304+P340+P312-P305+P351+P338 |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| F | 3-10-21 |
CLOSTRIPAIN price More Price(4)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | C0888 | Clostripain from Clostridium histolyticum ≥20?units/mgsolid | 9028-00-6 | 250units | $848 | 2024-03-01 | Buy |
| Alfa Aesar | J61362 | Clostripain, Clostridium histolyticum | 9028-00-6 | 1mg | $66 | 2023-06-20 | Buy |
| Alfa Aesar | J61362 | Clostripain, Clostridium histolyticum | 9028-00-6 | 10mg | $385 | 2023-06-20 | Buy |
| Usbiological | C5860 | Clostripain | 9028-00-6 | 5mg | $418 | 2021-12-16 | Buy |
CLOSTRIPAIN Chemical Properties,Uses,Production
Uses
A protease that cleaves proteins on the carboxyl bond of arginine
Uses
Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).
Biochem/physiol Actions
Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.
Purification Methods
Clostripain is isolated from Clostridium histolyticum callogenase by extraction in pH 6.7 buffer, followed by hydroxylapatite chromatography with a 0.1-0.2 M phosphate gradient, then Sephadex G-75 gel filtration with 0.05M phosphate pH 6.7, dialysis and a second hydroxylapatite chromatography (gradient elution with 0.1M 0.3M phosphate, pH 6.7). It has proteinase and esterase activity and is assayed by hydrolysing N-benzoyl-L-arginine methyl ester. [Mitchell & Harrington J Biol Chem 243 4683 1968, Methods Enzymol 19 635 1970.]
CLOSTRIPAIN Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| Hubei Ipure Biology Co., Ltd | +8613367258412 | ada@ipurechemical.com | China | 10326 | 58 |
| Career Henan Chemica Co | +86-0371-86658258 15093356674; | laboratory@coreychem.com | China | 30255 | 58 |
| HONG KONG IPURE BIOLOGY CO.,LIMITED | 86 18062405514 18062405514 | ada@ipurechemical.com | CHINA | 3465 | 58 |
| Shanghai Acmec Biochemical Technology Co., Ltd. | +undefined18621343501 | product@acmec-e.com | China | 33349 | 58 |
| Aladdin Scientific | +1-833-552-7181 | sales@aladdinsci.com | United States | 57511 | 58 |
| Aladdin Scientific | +1-833-552-7181 | sales@aladdinsci.com | United States | 52927 | 58 |
| Shanghai Baoman Biotechnology Co., Ltd. | 021-62130998 | baomanbio@163.com | China | 105 | 55 |
| J & K SCIENTIFIC LTD. | 010-82848833 400-666-7788 | jkinfo@jkchemical.com | China | 94838 | 76 |
| Adamas Reagent, Ltd. | 400-6009262 16621234537 | zhangsn@titansci.com | China | 14113 | 59 |
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