ChemicalBook > Product Catalog >Analytical Chemistry >Analysis reagents >Ion chromatography reagents >HEPES
HEPES
- CAS No.
- 7365-45-9
- Chemical Name:
- HEPES
- Synonyms
- HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
- CBNumber:
- CB5408557
- Molecular Formula:
- C8H18N2O4S
- Molecular Weight:
- 238.3
- MOL File:
- 7365-45-9.mol
- MSDS File:
- SDS
- Modify Date:
- 2026/1/27 8:46:37
| Melting point | 234-238 °C |
|---|---|
| Boiling point | 408℃[at 101 325 Pa] |
| bulk density | 560kg/m3 |
| Density | 1.07 g/mL at 20 °C |
| vapor pressure | 0Pa at 25℃ |
| refractive index |
n |
| storage temp. | 2-8°C |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| pka | 7.5(at 25℃) |
| PH Range | 7.0 - 7.6 |
| biological source | synthetic |
| Water Solubility | Soluble |
| λmax |
λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| Stability | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| Major Application | diagnostic assay manufacturing |
| Cosmetics Ingredients Functions | BUFFERING |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| SMILES | OCCN1CCN(CC1)CCS(O)(=O)=O |
| LogP | -3.85 at 20℃ |
| CAS DataBase Reference | 7365-45-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
| Absorption | ≤0.05 at 290 in H2O at 33% |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H302-H315-H319-H335 | |||||||||
| Precautionary statements | P261-P271-P280 | |||||||||
| PPE | Eyeshields, Gloves, type N95 (US) | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 24/25-22-36-26 | |||||||||
| WGK Germany | 1 | |||||||||
| RTECS | TL6809000 | |||||||||
| TSCA | TSCA listed | |||||||||
| HS Code | 29335995 | |||||||||
| Storage Class | 13 - Non Combustible Solids | |||||||||
| NFPA 704 |
|
HEPES price More Price(60)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | RDD002 | HEPES anhydrous, free-flowing, Redi-Dri?, ≥99.5% | 7365-45-9 | 1KG | ₹65361.35 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | RDD002 | HEPES anhydrous, free-flowing, Redi-Dri?, ≥99.5% | 7365-45-9 | 2.5KG | ₹107427.3 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | H7523 | HEPES BioXtra, pH 5.0-6.5 (1?M in H2O), ≥99.5% (titration) | 7365-45-9 | 50G | ₹11582.75 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | PHR1428 | HEPES Pharmaceutical Secondary Standard; Certified Reference Material | 7365-45-9 | 1G | ₹8346.08 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | H6147 | HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration) | 7365-45-9 | 25G | ₹6765.63 | 2022-06-14 | Buy |
HEPES Chemical Properties,Uses,Production
Description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemical Properties
White crystalline powder
Uses
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
Uses
HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay.
Definition
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
General Description
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biological Activity
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Purification Methods
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
HEPES Preparation Products And Raw materials
Global( 993)Suppliers
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| GLR Innovations | +91 9891111994 | New Delhi, India | 4535 | 58 | Inquiry |
| ARRAKIS INDUSTRIES LLP | +91 74995 32711 | Maharashtra, India | 1353 | 58 | Inquiry |
| JSK Chemicals | +919879767970 | Gujarat, India | 3756 | 58 | Inquiry |
| A.J Chemicals | 91-9810153283 | New Delhi, India | 6100 | 58 | Inquiry |
| CLEARSYNTH LABS LTD. | +91-22-45045900 | Hyderabad, India | 6257 | 58 | Inquiry |
| Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5870 | 58 | Inquiry |
| Alfa Aesar | 1 800 209 7001 | Maharashtra, India | 6905 | 58 | Inquiry |
| Triveni chemicals | 08048762458 | New Delhi, India | 6088 | 58 | Inquiry |
| LOBA CHEMIE PVT.LTD. | 91-22-6663 6699 | Mumbai, India | 3075 | 58 | Inquiry |
| Central Drug House(P) Ltd. | 91-11-49404040 | New Delhi, India | 6157 | 58 | Inquiry |
| Supplier | Advantage |
|---|---|
| GLR Innovations | 58 |
| ARRAKIS INDUSTRIES LLP | 58 |
| JSK Chemicals | 58 |
| A.J Chemicals | 58 |
| CLEARSYNTH LABS LTD. | 58 |
| Otto Chemie Pvt. Ltd. | 58 |
| Alfa Aesar | 58 |
| Triveni chemicals | 58 |
| LOBA CHEMIE PVT.LTD. | 58 |
| Central Drug House(P) Ltd. | 58 |
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- Jan 6，2020
7365-45-9(HEPES)Related Search:
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HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE
HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%
N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID))
Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz
1-HeptaneSulfonicAcidSod.SaltGr
N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid)
N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes)
Hepes,LowSodium,FreeAcid
HEPES free acid, DNase, Rnase and Protease free, for molecular biology, 99+%
Hydroxyethyl)-1-piperazineethyanesulfonic acid, 4-(2-
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid(HEPES)
2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid
HEPES, for biochemistry, 99%
4-(2-Hydroxyethyl)-1-Piperazin
Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid
1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
N-(2-hydroxymethyl)piperazine-N｀-(2-ethanesulfonic acid)(HEPES)
N-2-HYDROXYETHYLPIPERAZINE-N-2-ETHANSULFONICACID(HEPES)
4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid HEPES
HEPES / 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULPHONIC ACID
HEPES (4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID)
HEPES ULTRAPURE
N-2-HYDROXYETHYLPIPERAZINE-N''-2-ETHANE SULPHONIC ACID >99.5%
N-[2-HYDROXYERHYL]PIPERAZINE-N''-ETHANESULFONICACID(HEPES)
HEPES free acid ultrapure
N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid, low sodium >99.5%
HEPES(N-[2-Hydroxyethyl]piperazine-N'-[Ethanesulfonic acid]),Free Acid
N'-2-N-2-hydroxyethyl piperazineethanesulfonic acid
HEPES, >=99.5% (titration)
HEPES SynonyMs 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
HEPES,4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid
HEPES Vetec(TM) reagent grade, 99.5%
4-CHLORO-1-NAPHTHOL ULTRA PURE GRADE
POTASSIUM HYDROXIDE 0,05 MOL DUTY PAID
POTASSIUM HYDROXIDE 0,5MOL/L DUTY PAID
POTASSIUM HYDROXIDE 1 MOL/L DUTY PAID
SELENIUM STAND
MALATE DEHYDROGENASE ULTRA PURE GRADE
PHOSPHOTUNGSTIC ACID HIGH PURITY GRADE
4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
hepesfreeacid
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID
LABOTEST-BB LT00138109
LABOTEST-BB LT00233156
HEPES
HEPES-ACID
4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
hepes free acid molecular biology*reagent
HEPES N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
HEPES, 1M solution
HEPES, Free Acid, MB Grade (1.12041)
HEPES, Free Acid, ULTROL Grade
HEPES, Molecular Biology Grade N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade
HEPES, Tissue Culture Tested N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested
N-(2-HYDROXYETHYL)PIPERAZINE N-2-ETHANESULFONIC ACID
N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID