ピロニン 化学特性,用途語,生産方法
外観
暗緑色～暗褐色, 結晶性粉末～粉末
溶解性
エタノールおよび水に溶けやすい。
解説
ピロニン，ピロニンG(pyronine G)：C17H19ClN2O(302.80)．N,N-ジメチルアミノフェノールから合成される．緑色の結晶．水溶液は赤色を呈する．λmax 552 nm(エタノール)．【Ⅱ】ピロニンB(pyronine B)：C42H54Cl8Fe2N4O2(1042.24)．鉄との複合体で市販されている．緑色の針状晶．λmax 555 nm(エタノール)．
森北出版「化学辞典（第2版）
製造
細菌や核酸染色に用いられる色素で，ピロニンGとピロニンBがある．ピロニン染色とメチルグリーンによる染色は核酸の証明に不可欠な染色法である．
化学的特性
green crystalline solid
使用
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
定義
ChEBI: An organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna
Pappenheim method.
一般的な説明
Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.
純化方法
Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]
ピロニン 上流と下流の製品情報
原材料
準備製品