ブロモフェノールブルー (0.1%, 約50%エタノール溶液) 化学特性,用途語,生産方法
外観
黄色～赤色, 粉末
溶解性
水に殆ど不溶。エタノール, ベンゼン, 希水酸化ナトリウム溶液に可溶。エタノールにやや溶けやすく、水に溶けにくい。水酸化ナトリウム溶液に溶ける。
用途
弱塩基の中和滴定指示薬、変色点は酸性側(pH3.0～4.6)。
用途
pH指示薬, 電気泳動の先端マーカー色素。
特長
100ml包装は滴下用褐色ガラス瓶を使用しています。
化学的特性
Bromophenol Blue is pink or red powder, soluble in ethanol, benzene, ether and other organic solvents, slightly soluble in water, the aqueous solution is yellow, and blue-purple in alkaline solution. It is mainly used as an acid-base indicator, the pH discoloration range is 3.0 to 4.6, and the color changes from yellow to green to blue-violet.
製造方法
Bromophenol Blue is prepared by slow addition of excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.
Synthesis steps: Dissolve phenol red in glacial acetic acid, add a solution of bromine in glacial acetic acid with stirring, pour into 60°C hot water after stirring for a few minutes, cool to room temperature, and place overnight. Filter, wash the filter cake with glacial acetic acid and benzene in turn, and air dry to obtain bromophenol blue.
定義
ChEBI: Bromophenol blue is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3,5-dibromo-4-hydroxyphenyl groups. It is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as a size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis. It has also been used as an industrial dye. It has a role as a two-colour indicator, an acid-base indicator and a dye. It is a sultone, an arenesulfonate ester, a 2,1-benzoxathiole, a member of phenols and an organobromine compound.
純化方法
Crystallise the dye from *C6H6 or Me2CO/AcOH, and dry it in air. It is an indicator: at pH 3.0 it is yellow and it is purple at pH 4.6. [Beilstein 19/3 V 458.]
ブロモフェノールブルー (0.1%, 約50%エタノール溶液) 上流と下流の製品情報
原材料
準備製品