T4ジーン32プロテイン 化学特性,用途語,生産方法
使用
T4 Gene 32 Protein is a single-strand-specific DNA-binding protein. It may be used for:
- Optimization of PCR (See "Product Description" below.)
- Stimulation of in vitro DNA synthesis
- Stabilization of single-stranded regions of DNA or RNA
- Site-specific mutagenesis experiments (with T4 DNA polymerase and T4 DNA ligase)
- Helping restriction enzyme digestions go to completion
- q-PCR
一般的な説明
T4 Gene 32 Protein is reported to improve the yield of long PCR products when 0.5 to 1.0 nmol of the protein is included in the reaction. T4 Gene 32 Protein is also reported to increase the yield of PCR products amplified from samples that contain humic acid. The inhibitory effect of humic acid is reduced by a factor of 7 when T4 Gene 32 Protein is included in the PCR (at a concentration of 2.5 μg/100 μl).
The protein is isolated from the triple-mutant T4amN134/ amBL292/ amE 219 defective for the T4 genes 33, 35, and 58 to 61.
T4ジーン32プロテイン 上流と下流の製品情報
原材料
準備製品