リボフラビン5'-二りん酸P2-(5'-アデノシル)P1,P2-ジナトリウム 化学特性,用途語,生産方法
外観
うすい黄色～褐色粉末～結晶
定義
本品は、核酸誘導体のナトリウム塩であり、次の化学式で表される。
効能
ビタミンB2補充薬
商品名
フラビタン (トーアエイヨー); フラビタン (トーアエイヨー); フラビタン (トーアエイヨー); フラビタン (トーアエイヨー); フラビタン (トーアエイヨー)
化学的特性
yellow-orange powder
使用
The prosthetic group of certain flavoproteins including D-amino acid oxidase, glucose oxidase, glycine oxidase, fumaric hydrogenase, histaminase, and xanthine oxidase. Riboflavin kinase tumor necrosis factor receptor 1 NADPH oxidase.
純化方法
Small quantities of FAD are purified by paper chromatography using tert-butyl alcohol/water, cutting out the main spot and eluting with water. Larger amounts can be precipitated from water as the uranyl complex by adding a slight excess of uranyl acetate to a solution at pH 6.0, dropwise and with gentle stirring. The solution is set aside overnight in the cold, and the precipitate is centrifuged off, washed with small portions of cold EtOH, then with cold peroxide-free diethyl ether. It is dried in the dark under vacuum over P2O5 at 50-60o. The uranyl complex is suspended in water, and, after adding sufficient 0.01M NaOH to adjust the pH to 7, the precipitate of uranyl hydroxide is removed by centrifugation [Huennekens & Felton Methods Enzymol 3 954 1957]. It can also be crystallised from water. It should be kept in the dark. More recently it was purified by elution from a DEAE-cellulose (Whatman DE 23) column with 0.1M phosphate buffer pH 7, and the purity was checked by TLC. [Holt & Cotton, J Am Chem Soc 109 1841 1987, Beilstein 26 III/IV 3632.]
リボフラビン5'-二りん酸P2-(5'-アデノシル)P1,P2-ジナトリウム 上流と下流の製品情報
原材料
準備製品