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アルブミン, 牛血清製

アルブミン, 牛血清製 化学構造式
9048-46-8
CAS番号.
9048-46-8
化学名:
アルブミン, 牛血清製
别名:
アルブミン,ウシ;アルブミン, 牛血清製;ウシ血清由来アルブミン標準溶液;BOVINE ALBUMIN;アルブミン, ウシ血漿由来;アルブミン, ウシ血清由来, 粉末;アルブミン, ニワトリ, 卵白由来;アルブミン, ウシ血清由来, 脂肪酸不含;アルブミン, BOVINE;アルブミン, フラクションV, 細胞培養用, 脂肪酸フリー, ロW エンドトキシン;ALBUMIN SOLUTION (35%) FRACTIONV FROM BOVINE;ALBUMIN, ESSENTIALLY GLOBULIN-FREE ウシ血清由来;アルブミン ウサギ血清由来;アルブミン ウシ血清由来;アルブミン ヒツジ血清由来;アルブミン ブタ血清由来;アルブミン マウス血清由来;アルブミン ヤギ由来;アルブミン ラット血清由来;アルブミン, アセチル化 ウシ血清由来
英語化学名:
Bovine albumin
英語别名:
nhsa;BSAV;BSA-C;AC-BSA;aminofusin;ALBUMIN, PIG;albumenpowder;SERUM ALBUMIN;Albumin equine;BSA ACETYLATED
CBNumber:
CB5107573
化学式:
N/A
分子量:
0
MOL File:
Mol file

アルブミン, 牛血清製 物理性質

RTECS 番号:
MT6446000
貯蔵温度 :
2-8°C
溶解性:
PBS: >40 mg/mL
外見 :
lyophilized powder
色:
clear clear, colorless
PH:
6.5-7.5 (1% in 0.15 M sodium chloride)
水溶解度 :
Soluble in water.
Merck :
13,8542
安定性::
Stable. Incompatible with strong oxidizing agents and strong acids. Refrigerate.
EPAの化学物質情報:
Albumins, blood serum(9048-46-8)
安全性情報
  • リスクと安全性に関する声明
  • 危険有害性情報のコード(GHS)
主な危険性  Xn
Rフレーズ  22-52/53-21/22
Sフレーズ  24/25-61-36/37
WGK Germany  3
10-21
TSCA  Yes
HSコード  35029000
絵表示(GHS)
注意喚起語
危険有害性情報
コード 危険有害性情報 危険有害性クラス 区分 注意喚起語 シンボル P コード
H302 飲み込むと有害 急性毒性、経口 4 警告 P264, P270, P301+P312, P330, P501
注意書き
P264 取扱い後は皮膚をよく洗うこと。
P264 取扱い後は手や顔をよく洗うこと。
P270 この製品を使用する時に、飲食または喫煙をしないこ と。
P330 口をすすぐこと。

アルブミン, 牛血清製 価格 もっと(296)

メーカー 製品番号 製品説明 CAS番号 包装 価格 更新時間 購入
富士フイルム和光純薬株式会社(wako) W01MPB02152401 アルブミン, ウシ血清由来, 脂肪酸不含
Albumin, from Bovine Serum, Fatty Acid Free
9048-46-8 5g ¥8500 2018-12-26 購入
富士フイルム和光純薬株式会社(wako) W01MPB02103703 アルブミン, ウシ血清由来, 粉末
Albumin, from Bovine Serum, Powder
9048-46-8 100g ¥95200 2018-12-26 購入
東京化成工業 T3796 ウシ血清由来アルブミン標準溶液
Standard Solution of Albumin from Bovine Serum
9048-46-8 5mL ¥6000 2018-12-04 購入
Sigma-Aldrich Japan A0281 アルブミン ウシ血清由来
Bovine Serum Albumin
lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis)
9048-46-8 100mg ¥6900 2018-12-25 購入
Sigma-Aldrich Japan 1076192 United States Pharmacopeia (USP) Reference Standard
BSA for protein quantitation United States Pharmacopeia (USP) Reference Standard
9048-46-8 5x1ml ¥150000 2018-12-25 購入

アルブミン, 牛血清製 MSDS


Bovine serum albumin

アルブミン, 牛血清製 化学特性,用途語,生産方法

定義

本品は、血漿から得られるタンパク又はタンパク分画である。

化粧品の成分用途

ヘアコンディショニング剤、皮膚コンディショニング剤、皮膜形成剤

化学的特性

Crystalline powder

使用

Suitable for cell culture media supplement.

酵素阻害剤

This abundant blood protein (MW = 66,463 Da; CAS 9048-46-8) regulates osm2o+tic p+ressur+e and serves as a binding protein for fatty acid hormone, Ca, K, Na, bilirubin, and many hydrophobic and amphipathic pharmaceuticals. The ease of purification made led to its use as an protein standard in biochemistry. BSA is frequently used (at 1–2 mg/mL) to stabilize proteins and enzymes by exerting what is called the protective colloid effect. BSA often protects against dilution-induced dissociation/inactivation of susceptible. BSA also reduces enzyme adsorption to the surfaces of plastic labware. Because BSA binds a wide variety of substances (including substrates, cofactors, or other components of the reaction mixture), it may inhibit enzyme activity, and one should run control experiments before using BSA indiscriminantly. 1. Purich Enzyme Kinetics: Catalysis & Control, Academic Press (Elsevier), New York. Albuterol, See Salbutamol Alcapton, See Homogentisate and Homogentisic Acid Aldehyde-Containing Protease Inhibitors The catalytic mechanisms of serine and cysteine proteases are multi-step catalytic process, of which a key step is the formation of oxygen- or thiol- ester intermediate. Nested around that intermediate is a manifold of transitory species, including any tetrahedral adducts leading to and from that covalent acyl-enzyme. Papain, for example, is a thiol proteinase that is strongly inhibited by aldehydes resembling carboxylic acids that are released upon enzyme-catalyzed hydrolysis of specific substrates. Westerik & Wolfenden found that at pH 5.5, approximately one mol acetyl-L- phenylalanylamino-acetaldehyde is bound per mol papain (Ki = 4.6 x 10–8 M). These aldehydes protect the Enzyme-SH against inactivation by the thiol-modifying reagent N-ethylmaleimide, presumably as a consequence of the enzyme forming an unusually stable thiohemiacetal with stereoelectronic features resembling tetrahedral intermediates in enzyme- catalyzed substrate hydrolysis. Such ideas have guided the design of countless peptide aldehydes that have proved to be especially effective inhibitors. The objective is to achieve specificity by capitalizing on knowledge of a target enzyme’s preference for side-chains in the peptide substrate, while relying on reversible formation of hemiacetal or hemithioacetal adducts to gain potency.

純化方法

Albumin is purified by dissolving it in conductivity water and passage at 2-4o through two ion-exchange columns, each containing a 2:1 mixture of anionic and cationic resins (Amberlite IR-120, H-form, Amberlite IRA-400, OH -form). This treatment removes ions and lipid impurities. Care is taken to exclude CO2, and the solution is stored at -15o. [M.ller et al. Trans Faraday Soc 57 312 1961.] More complete lipid removal is achieved by lyophilising the de-ionised solution, covering the dried albumin (human serum) with a mixture of 5% glacial acetic acid (v/v) in iso-octane (previously dried with Na2SO4) and allowing it to stand at 0o (without agitation) for upwards of 6hours before decanting and discarding the extraction mixture, washing with iso-octane, re-extracting, and finally washing twice with iso-octane. The purified albumin is dried under vacuum for several hours, then dialyzed against water for 12-24hours at room temperature, lyophilised, and stored at -10oC [Goodman Science 125 1296 1957]. It has been recrystallised in high (35%) and in low (22%) EtOH solutions from Cohn's Fraction V. The high EtOH recrystallisation is as follows: To 1kg of Fraction V albumin paste at -5o is added 300mL of 0.4 M pH (pH 5.5) acetate buffer in 35% EtOH pre-cooled to -10o and 430 mL of 0.1 M NaOAc in 25% EtOH also at -10o. Best results are obtained by adding all of the buffer and about half of the NaOAc and stirring slowly for 1hour. The rest of the NaOAc is added when all the lumps have disintegrated. The mixture is set aside at -5o for several days to crystallise. 35% EtOH (1 L) is then added to dilute the crystalline suspension and lower the ionic strength prior to centrifugation at -5o (yield 80%). The crystals are further dissolved in 1.5 volumes of 15% EtOH/0.02M NaCl at -5o and clarified by filtration through washed, calcined diatomaceous earth. This solution may be recrystallised by re-adjusting to the conditions in the first crystallisation, or it may be recrystallised at 22% EtOH with the aid of a very small amount of decanol (enough to give a final concentration of 0.02%). Note that crystallisation from lower EtOH concentration gave better purification (i.e. by removing globulins and carbohydrates) and producing a more stable product. The low EtOH recrystallisation is as follows: To 1kg of Fraction V at -10o to -15o is added 500mL of 15% EtOH at -5o, stirred slowly until a uniform suspension is formed. To the 15% EtOH (500mL) is added sufficient 0.2M NaHCO3 solution (125-150mL) at 0o to bring the pH (1:10 dilution) to 5.3. Some temperature rise occurs, and care must be taken to keep the temperature < -5o. If the albumin is incompletely dissolved a small amount of H2O is added (100mL at a time at 0o, allowing 15minutes between additions). Undissolved albumin can be easily distinguished from small amounts of undissolved globulins, or as the last albumin dissolves, the appearance of the solution changes from milky white to hazy grey-green in colour. Keep the solution at -5o for 12hours and filter by suspending in 15g of washed fine calcined diatomaceous earth, and filtering using a Büchner funnel precoated with coarser diatomaceous earth. The filtrate may require two or more similar filtrations to give a clear solution. To crystallise the filtrate, add through a capillary pipette, and with careful stirring, 1/100volume of a solution containing10% decanol and 60% EtOH (at -10o), and seed with the needle-type albumin crystals. After 2-3days, crystallisation is complete. The crystals are centrifuged off. These are suspended with gentle mechanical stirring in one-third their weight of 0.005 M NaCl pre-cooled to 0o. With careful stirring, H2O (at 0o) is added slowly in an amount equal to 1.7 times the weight of the crystals. At this stage there is about 7% EtOH, and the temperature cannot be made lower than -2.5o to -1o. Clarify, and collect as above. [Cohn et al. J Am Chem Soc 69 1753 1947.] Human serum albumin has been purified similarly with 25% EtOH and 0.2% decanol. The isoelectric points of bovine and human serum albumins are 5.1 and 4.9, respectively.

アルブミン, 牛血清製 上流と下流の製品情報

原材料

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アルブミン, 牛血清製 生産企業

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9048-46-8(アルブミン, 牛血清製)キーワード:


  • 9048-46-8
  • ALBUMIN, BOVINE FRACTION V POWDERCELL CU LTURE TEST
  • ALBUMIN, BOVINE FRACTION V POWDERLOW END OTOXIN
  • ALBUMIN, PIG GLOBULIN-FREE
  • ALBUMIN, BOVINE FRACTION V POWDEREMBRYO TESTED
  • ALBUMIN BOVINE 25% SOLUTION IN TYRODE'S BUFFER
  • ALBUMIN RHESUS MONKEY FRACTION V POWDER
  • ALBUMIN, PIG, FATTY ACID FREE, GLOBULIN FREE
  • ALBUMIN BOVINE 5% SOLUTION
  • ALBUMIN BOVINE 22% SOLUTION
  • ALBUMIN, GOAT FATTY ACID FREE
  • ALBUMIN, RAT ESSENTIALLY GLOBULIN FREE
  • ALBUMIN, BOVINE, CELL CULTURE TESTED
  • ALBUMIN FROM RABBIT SERUM, FRACTION V*
  • ALBUMIN, SHEEP, FRACTION V POWDER
  • ALBUMIN, BOVINE SERUM 10% SOLUTION CELL
  • ALBUMIN, MOUSE GLOBULIN FREE
  • ALBUMIN, BOVINE FRACTION V POWDER FATTY ACID FREE,
  • ALBUMIN BOVINE FRACTION V POWDERCELL CUL TURE TESTE
  • ALBUMIN, BOVINE 30% SOLUTION HIGHAVIDITY SUBSTANTI
  • ALBUMIN, BOVINE 35% SOLUTION
  • ALBUMIN BOVINE ESSENTIALLY GLOBULINFREE CELL CULTU
  • PROTEIN STANDARD (BOVINE SERUM ALBUMIN) 2 MG/VIAL
  • ALBUMIN, BOVINE, GLOBULIN FREE
  • ALBUMIN, RAT, FATTY ACID FREE, GLOBULIN FREE
  • BOVINE SERUM ALBUMIN MOLECULAR BIOLOGYRE AGENT AQUE
  • ALBUMIN, BOVINE, ESSENTIALLY IGG FREE
  • ALBUMIN BOVINE FRACTION V 35% SOLUTION&
  • ALBUMIN PIG FRACTION V POWDER
  • ALBUMIN HORSE FRACTION V POWDER
  • ALBUMIN BOVINE RIA GRADE FRACTIONV POWDE R
  • アルブミン,ウシ
  • アルブミン, 牛血清製
  • ウシ血清由来アルブミン標準溶液
  • BOVINE ALBUMIN
  • アルブミン, ウシ血漿由来
  • アルブミン, ウシ血清由来, 粉末
  • アルブミン, ニワトリ, 卵白由来
  • アルブミン, ウシ血清由来, 脂肪酸不含
  • アルブミン, BOVINE
  • アルブミン, フラクションV, 細胞培養用, 脂肪酸フリー, ロW エンドトキシン
  • ALBUMIN SOLUTION (35%) FRACTIONV FROM BOVINE
  • ALBUMIN, ESSENTIALLY GLOBULIN-FREE ウシ血清由来
  • アルブミン ウサギ血清由来
  • アルブミン ウシ血清由来
  • アルブミン ヒツジ血清由来
  • アルブミン ブタ血清由来
  • アルブミン マウス血清由来
  • アルブミン ヤギ由来
  • アルブミン ラット血清由来
  • アルブミン, アセチル化 ウシ血清由来
  • タンパク質標準品
  • ウシ血清アルブミン
  • 血清タンパク
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