エチジウムブロミド 化学特性,用途語,生産方法
外観
赤色〜暗赤紫色, 結晶性粉末〜粉末
溶解性
水に溶ける。
解説
核酸の2塩基分子間にインターカレーションではいり込みやすい構造の有機色素.C
21H
20BrN
3.ゲル電気泳動をした核酸の染色に利用する.臭化エチジウム固有の光吸収(ピーク300 nm)や,核酸の光吸収(ピーク260 nm)とエネルギー移動を経て現れる蛍光(ピーク590 nm)を観測することにより,核酸のバンドを検出できる.
化学的特性
Purple/maroon crystalline powder
使用
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml).
Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).
健康ハザード
Acute toxic effects from exposure to ethidium bromide have not been thoroughly
investigated. Ethidium bromide is irritating to the eyes, skin, mucous membranes,
and upper respiratory tract.
Although there is no evidence for the carcinogenicity or teratogenicity of this
substance in humans, ethidium bromide is strongly mutagenic and therefore should
be regarded as a possible carcinogen and reproductive toxin
燃焼性と爆発性
Ethidium bromide does not pose a flammability hazard (NFPA rating = 1).
安全性プロファイル
Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes.
貯蔵
Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Operations capable of generating ethidium bromide dust or aerosols of ethidium bromide solutions should be conducted in a fume hood to prevent exposure by inhalation.
純化方法
Crystallise it from MeOH or EtOH [Lamos et al. J Am Chem Soc 108 4278 1986]. Its solubility in H2O is 1%. [Beilstein 22/11 V 352.] POSSIBLE CARCINOGEN.
不和合性
No incompatibilities are known.
廃棄物の処理
Excess ethidium bromide and waste material containing this substance should be placed in an appropriate container, clearly labeled, and handled according to your institution's waste disposal guidelines.
エチジウムブロミド 上流と下流の製品情報
原材料
準備製品