D-ルシフェリンナトリウム一水和物 化学特性,用途語,生産方法
外観
わずかにうすい黄色〜うすい褐色, 結晶性粉末〜粉末
溶解性
水に溶ける。
用途
ATPの定量、クレアチンキナーゼやヌクレオチドホスファターゼ等の活性測定。
説明
D-
Luciferin is a chemiluminescent substrate of firefly luciferase. It produces light upon oxidative decarboxylation by luciferase in the presence of ATP. D-
luciferin can be employed to assay the expression of the luciferase gene linked to a promoter of interest. Alternatively, D-
luciferin and luciferase can be used to assess ATP availability in cellular or biochemical assays.
化学的特性
Yellow powder
使用
D-Luciferin sodium salt is substrate for firefly luciferase, properties and use in assays of ATP and luciferase.
一般的な説明
D-Luciferin is mainly produced in bioluminescent organisms. It can simply circulate across the blood testis barrier, blood brain barrier and the blood placenta barrier. This small molecule is sensitive to light and oxygen. D-Luciferin is also sensitive to moisture in its powder form.
生物活性
D-Luciferin sodium salt is a firefly luciferase substrate (Km = ~2 μM). Used in much standard bioluminescence imaging (BLI) techniques, including monitoring tumor growth and investigating intracellular signaling activity in vitro and in vivo. Cell permeable. Emission maximum = 562 nm.
D-ルシフェリンナトリウム一水和物 上流と下流の製品情報
原材料
準備製品